International Journal of Advanced Biological and Biomedical Research

Evaluation of Antimicrobial Activity of Rhazya Stricta (Apocynaceae) Extract Prepared with Different Solvents on Staphylococcus Aureus (Staphylococcaceae) Isolated from Humans

Maryam Beigomi; Laleh Shahraki-Mojahed; Batool Heydari-Sadegh; Narjes Dahmardeh; Reza Rouhani; Fereshteh Javadian

Volume 9, Issue 3 , September 2021, , Pages 241-253

https://doi.org/10.22034/ijabbr.2021.528883.1357

Abstract

Introduction: Bacterial resistance to antibiotics, the first major concern in the 1960s, has reappeared worldwide over the past 20 years. Because these bacteria are not resistant to ... Read More Introduction: Bacterial resistance to antibiotics, the first major concern in the 1960s, has reappeared worldwide over the past 20 years. Because these bacteria are not resistant to various conventional therapies, the medicinal and herbal plants used in different countries should be evaluated for their therapeutic potential. These valuable biological resources are a repository of complex active molecules. Therefore, in this study, we tried to evaluate the antimicrobial activity of some medicinal plant extracts on Staphylococcus aureus isolated from humans.Methods: Rhazya stricta was collected and their species were identified in the botanical laboratory of University of Zabol. To prepare plant extract, 40 g of dried leaves were used in 400 cc of solvent (aqueous, ethanol, methanol, ethyl acetate, and hydro-alcoholic). The various strains of Staphylococcus aureus used in this study were isolated from the human nose and identified by biochemical, bacteriological and growth tests as well as standard tests. Antimicrobial effects were investigated by well diffusion method in Müller Hinton agar medium. Statistix ver10 software was used for statistical calculations. Mean comparison was performed using the LSD at the level of one percent and Excel software was also used to draw the shapes.Results: Rhazya stricta extracts had different inhibitory zone diameters against Staphylococcus aureus at 100 ppm dilution (p <0.01). R. stricta ethanolic extract had the greatest effect (average 8.3 mm) on inhibiting the growth of 6 strains of S. aureus. Then aqueous and hydroalcoholic extracts with an average of 7 mm were in the next ranks. The ethyl acetate extract had the lowest effect on the inhibition of S. aureus. The lowest MIC of R. stricta ethanolic extract against S. aureus samples was 3.1 ppm, which was inhibited by four strains. The lowest MBC was 6.2 ppm.Conclusion: Considering the side effects of chemical drugs and antibiotics as well as the potential effect of ethanolic extract of Rhazya stricta on Staphylococcus aureus, it is recommended to use ethanol solvent to evaluate the antimicrobial activity of R. stricta.

Antibacterial effects of silver nanoparticlesagainst resistant strains of E.coli bacteria

Saeide Saeidi; Fereshteh Javadian; Zahra Sepehri; Zahra Shahi; Fahime Mousavi; Mahmood Anbari

Volume 4, Issue 1 , March 2016, , Pages 96-99

https://doi.org/10.26655/ijabbr.2016.2.12

Abstract

The aim study antibacterial effects of silver nanoparticles against resistant strains of E.coli bacteria.12 strains of E. coli strains arising urinary infection were isolated from hospitalized ... Read More

Antibacterial activity of silver Nanoparticles produced by Rosmarinus officinalis L leaf extract against some human pathogenic bacteria

Saeide Saeidi; Fereshteh Javadian; Zahra Sepehri; Zahra Shahi; Razieh Farazmand; Mahmood Anbari

Volume 4, Issue 1 , March 2016, , Pages 108-111

https://doi.org/10.26655/ijabbr.2016.2.15

Abstract

The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. The aim of this study was to determined antibacterial activity ... Read More

Study the Effect of Ethanol Extract of Achillea, Green Tea and Ajowan on Pseudomonas Aeruginosa

Roghayeh Mohammadpour Vashvaei; Zahra Sepehri; Mehran Jahantigh; Fereshteh Javadian

Volume 3, Issue 2 , June 2015, , Pages 145-148

Abstract

Objective: Infections are increasing caused by antibiotic-resistant Pseudomonas aeruginosa day by day. For this reason, many researchers have tried to find new compounds as alternative ... Read More

Antimicrobial Activities of Teucrium Polium Against Salmonella Typhimurium

Roghayeh Mohammadpour Vashvaei; Zahra Sepehri; Mehran Jahantigh; Fereshteh Javadian

Volume 3, Issue 2 , June 2015, , Pages 149-152

Abstract

Objective: Teucrium polium L. (family Lamiaceae) is a wild-growing flowering plant, found abundantly in South-Western Asia, Europe and North Africa. Traditionally, T. polium has been ... Read More

Antimicrobial Impact of Hibiscus Sabdariffal Extract Against Acinetobacterbaumannii

Nagmeh Eskandary; Saeide Saeidi; Gelareh Sohil Baigi; Fereshteh Javadian

Volume 3, Issue 2 , June 2015, , Pages 172-175

Abstract

Objective: Antibiotic resistant to Antimicrobial agent is one of the most important concern in hospitals, which can cause lots of costs, treatment fails and mortality rates. Acinetobacter ... Read More

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Author = Fereshteh Javadian

Evaluation of Antimicrobial Activity of Rhazya Stricta (Apocynaceae) Extract Prepared with Different Solvents on Staphylococcus Aureus (Staphylococcaceae) Isolated from Humans

Abstract

Antibacterial effects of silver nanoparticlesagainst resistant strains of E.coli bacteria

Abstract

Antibacterial activity of silver Nanoparticles produced by Rosmarinus officinalis L leaf extract against some human pathogenic bacteria

Abstract

Study the Effect of Ethanol Extract of Achillea, Green Tea and Ajowan on Pseudomonas Aeruginosa

Abstract

Antimicrobial Activities of Teucrium Polium Against Salmonella Typhimurium

Abstract

Antimicrobial Impact of Hibiscus Sabdariffal Extract Against Acinetobacterbaumannii

Abstract