@article { author = {Ismail, Seham Mansour}, title = {Resistance of Egyptian Field Populations of Spodoptera littoralis (Lepidoptera: Noctuidae) to Emamectin Benzoate and Role of Detoxification Enzymes}, journal = {International Journal of Advanced Biological and Biomedical Research}, volume = {10}, number = {4}, pages = {277-287}, year = {2022}, publisher = {Sami Publishing Company}, issn = {2383-2762}, eissn = {2322-4827}, doi = {10.22034/ijabbr.2022.562491.1418}, abstract = {Background: One of the most significant pests that harm numerous agricultural crops ‎is the cotton leafworm Spodoptera littoralis (Boisduval) and it has evolved resistance ‎to various insecticides across most chemical classes, including the novel insecticide ‎emamectin benzoate. ‎Methods: The sensitivity of emamectin benzoate was evaluated in Egyptian cotton ‎leafworm field populations. ‎Results: According to bioassays performed using the leaf-dipping method, emamectin ‎benzoate sensitivity varied among 5 field populations, with LC50 values ranging from ‎‎2.78 to 6.86 ppm. Two S. littoralis populations demonstrated the low levels of ‎resistance at resistance ratios (RR) between 17.9- and 22.3-fold and three populations ‎showed moderate resistance at 33.5 to 42.8-fold compared with a susceptible ‎population. The findings demonstrated that diethyl maleate (DEM) and piperonyl ‎butoxide (PBO) have considerable synergistic effects on the toxicity of emamectin ‎benzoate. ‎Conclusion: The results suggest that emamectin benzoate resistance in the S. littoralis ‎populations studied may be caused by the enzyme systems monooxygenase (MO) and ‎glutathione S-transferase (GST).‎Highlights‎Emamectin benzoate efficacy is decreasing in populations of Spodoptera littoralis.‎‎Emamectin benzoate resistance in littoralis field populations ranges from low to ‎moderate. ‎‎The field populations' monooxygenase and glutathione S-transferase activity were ‎higher than those of the susceptible population‎.}, keywords = {Carboxylesterase,Emamectin benzoate resistance,Glutathione S-transferases,Monooxygenase,Synergism}, url = {https://www.ijabbr.com/article_696746.html}, eprint = {https://www.ijabbr.com/article_696746_3659456e55ba67b81523d0bbd4e89cff.pdf} }