%0 Journal Article %T Alfa-Glucosidase inhibitory and antioxidant activity of hexane extract of flowers, leave and stems of Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech. %J International Journal of Advanced Biological and Biomedical Research %I Sami Publishing Company %Z 2383-2762 %A Zarei, Mohammad %A Ghafaryan, Hero %D 2020 %\ 06/01/2020 %V 8 %N 2 %P 153-164 %! Alfa-Glucosidase inhibitory and antioxidant activity of hexane extract of flowers, leave and stems of Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech. %K Alpha-glucosidase %K Ferula haussknechtii %K Haplophyllum acutifolium %K diabetes %K Enzymatic inhibition %R 10.33945/SAMI/IJABBR.2020.2.6 %X Background:α-Glucosidase Inhibition can significantly prevent glucose uptake after meal, and helps in controlling of some adverse effects in diabetics. So determination of α-Glucosidase inhibitory effect and antioxidant activity of the Haplophyllum acutifolium and Ferula haussknechtii aerial organs was the aim of this study.Methods:Inhibitory effect of hexane extracts from different organs was investigated in several concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of hexane extracts of various organs was also measured using DPPH and iron reduction tests. Results;The highest inhibitory activity of F.haussknechtii was observed at the 0.1 g/ml concentration of flower extract (100% inhibition and IC50= 0.1 μg /ml) and the most inhibitory activity of H.acutifolium, was related to the 1 g/ml concentration of flower extract (100% inhibition and IC50 = 10 μg /ml) and leaf extract (100% inhibition and IC50 = 60 μg /ml). Extract of H.acutifolium flower and leaves showed Non-competitive inhibition pattern and F.haussknechtii flower showed mixed (Competitive -Non-Competitive) inhibitory pattern at 0.001 g/ml and exhibit uncompetitive inhibitory pattern at the 0.1 g/ml. The results of antioxidant potential showed EC50 for F.haussknechtii flower and H.acutifolium leaves equalled 2.37 and 0.96 mg/ml, respectively. Conclusions:The hexane extract of the F.haussknechtii flower, and H.acutifolium flower and leaf organ have a significant inhibitory effect on the activity of α-Glucosidase, DPPH free radical scavenging activities and reducing power. So they are good resources for extraction of medicinal compounds to control blood level of glucose after meal, in diabetic patients. %U https://www.ijabbr.com/article_37040_a961bb1129e0b09532a2e416598d46d3.pdf