TY - JOUR ID - 7972 TI - Parentage Tests in Khuzestan Buffaloes Populations Using Three Microsatellite Markers JO - International Journal of Advanced Biological and Biomedical Research JA - IJABBR LA - en SN - 2383-2762 AU - Banani Rad, Homayoun AU - Fayazi, Jamal AU - Amirinia, Cyrus AU - Roshanfekr, Hedayatolah AU - Seydabadi, Hamidreza AD - Department of Animal Science, Ramin Agriculture and Natural Resources University, Khouzestan, Iran AD - Department of Biotechnology, Animal Science Research Institute, Iran AD - Department of Biotechnology, Animal Science Research Institute,Iran Y1 - 2013 PY - 2013 VL - 1 IS - 11 SP - 1414 EP - 1422 KW - Parentage testing KW - identification KW - khouzestan buffaloes population KW - Microsatellite markers DO - N2 - Pedigree information and phenotypic records are the key features in animal genetics and breeding. Otherwise best methods for genetic evaluation and estimation of parameters will be tending to no correct results. This study was aimed to investigate and verify pedigree relations among 76 individuals who were genotyped for 3 fluorescent labeled microsatellite markers picked up from FAO-ISAG joint recommended list as one multiplex PCR set. 11 sires, 11offspring and 11 dams with 43 unknown samples were analyzed. Based on exact allele sizes, 33 alleles were observed within the studied population. The mean number of alleles per locus was 11. Polymorphism information content (PIC) values ranged from 0.3257 to 0.8043 with the mean value of 0.6213. The most informative loci were :BM2113 (16alleles,PIC=0.7338), BM1818(3alleles,PIC=0.3257).The indices of genetic variability within population and polymorphism of loci including average heterozygosis, the number of observed and effective alleles, polymorphic information content (PIC) and so on showed that the studiedĀ  khouzestan buffaloes population and used markers have still a reliable diversity and polymorphism level, respectively. Also, combined probability of exclusion (PE) values obtained per all loci in both parentage and identification analysis was 0.9381 and 0/4483thatindicate the high efficiency of studied marker set for parentage and identification test in this population UR - https://www.ijabbr.com/article_7972.html L1 - https://www.ijabbr.com/article_7972_5c19ea88613210ad87fa4c51a6789ce7.pdf ER -