Document Type : Original Article

Authors

1 Ethiopian Institute of Agricultural Research (EIAR), Wondo Genet Agricultural Research Center. P. O. Box 198, Shashemene, Ethiopia

2 Ethiopian Institute of Agricultural Research (EIAR), Debre Zeit Agricultural Research Center P.O.Box 32, Debre Zeit, Ethiopia

Abstract

Objective: Evaluation activity was conducted using three spearmint genotypes (WG-SPM-Engl, SPM-Fran and SPM-EAS) and one standard check (WGSM-03) at Wondo Genet, Awassa, Debre Zeit and Allagae during 2012 and 2013 for two years. Methods: The treatments were arranged in randomized complete block design with four replications. Data on leaf to stem ratio, leaf yield/ha, Essential oil content and essential oil yield were collected and statistically tested. The combined analysis of variance over the testing locations and years indicated the existence of significant variation among spearmint genotypes for all the characters considered in this study. Results: The respective leaf to stem ratio, leaf yield/ha, essential oil content and essential oil yield of the tested genotypes ranged from 1.29 to 2.2, 10.82 to 13.45 t, 0.16 to 0.5% and 18.13 to 67.99 kg, respectively. The highest leaf to stem ratio (2.2) was demonstrated by WG-SPM-Engl and the lowest (1.29) by WG-SPM-Fran. The highest leaf yield/ha was demonstrated by WG-SPM-Fran followed by WG-SPM-Engl; while the lowest value for these agronomic characters were produced by WG-SM03. Compared with WGSM 03, WG-SPM-Fran demonstrated a respective percent increase value of 47.05 and 81.45% on essential oil content and essential oil yield. The overall highest value for essential oil content (0.39%) was recorded at Allagae followed by Wondo Genet (0.35%) and Hawassa (0.34%); while the least value was recorded at Debre Zeit (0.31%). Averaged over the testing locations, the highest essential oil yield/ha (48.29 kg) was recorded at Hawassa and the lowest at Wondo Genet (34.85 kg). Compared with second testing year, a respective percent increase value of 12.12 and 23.18% on essential oil content and essential oil yield was demonstrated in first testing year.

Keywords

Abbaszadeh B, Alireza SV, Aliabadi HF, Hasanpour HD (2009). Studying of essential oil variations in leaves of Mentha species. African Journal of Plant Science 3 (10):217-221.
Aflatuni A (2005). The yield and essential oil content of mint (Mentha spp.) in Northern Ostrobothnia. PhD thesis, University of Oulu, Finland.
Beemnet M, Getinet A (2010). Variability in Ethiopian Coriander Accessions for Agronomic and Quality Traits. African Journal of Crop Science 18(2): 43-49.
Beemnet MK, Solomon A, Zinash TA, Haileslassie GK, Beniyam Y, Gizachew A, Bekri M, Wossen KM, Texeria DS (2011). Performance of Lemongrass (Cymbopogon
citratus L. (DC) Stapf) for Agronomic and Chemical Traits in Different Agro-Ecologies of Ethiopia. Medicinal and Aromatic Plant Science and Biotechnology 5(2): 133-138.
Belay B (2007). Assessment of crop growth and artemisinin content of the medicinal plant Artemisia annua Anamed in the Gamo highlands of SW Ethiopia. MSc. Thesis, University of Hohenheim, Sttutgart,
Germany.
Chalchat JC, Garry RP, Michet A (1997). Variation of the chemical composition of essential oil of Mentha2710 Mengesha Kassahun et al/ Int. J. Adv. Biol. Biom. Res, 2014; 2 (10), 2704-2711 
peperriata L. during the growing time. Journal of Essential Oil Research 9: 463-465.
Edris AE, Shalaby AS, Fadel MA, Wahab A (2003). Evaluation of a chemotype of spearmint (Mentha spicata L.) grown in Siwa Oasis, Egypt. European Food
Technology 218: 74-78.
El-Wahab, Mohamed A (2009). Evaluation of Spearmint (Mentha spicata L.) Productivity Grown in Different Locations under Upper Egypt Conditions. Research Journal of Agriculture and Biological Sciences 5(3): 250-254.
Fahmy IR, Saad SN, Ahmed ZF (1955). Effect of environment on yield and quality of the oil of Mentha piperita Grown in Egypt. chemical Abstract 51:8379-80.
Farooqi AHA, Mishra A, Naqvi AA (1983). Effects of plant age on quality of oil in Japanese mint. Indian Perfumer 27: 80-82.
Fehr WR (1991). Principles of Cultivar Development Theory and Technique. Iowa State University, USA, pp. 247-260.
Foda IM, El-Sayed MA, Hassan AA, Rasmy NM, ElMoghazy MM, (2010). Effect of Spearmint Essential Oil on Chemical Composition and Sensory Properties of White Cheese. Journal of American Science 6(5): 272-279. Frankel OH, Brown AHD, Burdon JJ (1994). The Conservation of Plant Diversity. Cambridge University Press, UK, pp. 22-29.
Gasic O, Mitica-Dukic N, Adamovic D, Borojevic K (1989). Variability of content and composition of essential oil in different genotypes of peppermint. Biochemical Systematics and Ecology 15: 335-340.
Gomez KA, Gomez AA (1984). Statistical Procedures for Agricultural Research (2nd Ed). John Wiley and Sons, New York, pp. 1-680.
Guenther E (1972). The Essential Oils: History-Origin in Plants Production-Analysis (Vol I). Robert E. Kriger Publishing Co., Malabar, Florida, 427p.
Hua, C.X., Wang, G.R. and Lei, Y. 2011. Evaluation of essential oil composition and DNA diversity of mint resources from China. African Journal of Biotechnology, 10(74): 16740-16745.
I.R.R.I (International Rice Research Institute) (1996): Plant Adaptation and Crop Improvement. CAB International, Manila, Philippines, pp. 3-5.
Kizil S, TonÁer ÷ (2006). Influence of different harvest times on the yield and oil composition of spearmint (Mentha spicata L. var. spicata). Journal of Food,
Agriculture and Environment 4 (3 and 4): 135-137.
Langston R, Leopold C (1954). Proc. Amer. Soc. Hort. Science 63: 347.
Salim EA, Gaafer M, Hassan E, Subki HEK (2014). Effect of Spacing and Seasonal Variation on Growth Parameters, Yield and Oil Content of Mint Plants. Journal of Forest Products and Industies 3(2), 71-74.
SAS (Statistical Analysis System) (2002). SAS/STAT. Guide Version 9. SAS, Institute Inc. Raleigh, North Carolina, USA
Shormin T, Khan MAH and Alamgir M (2009). Response of Different Levels of Nitrogen Fertilizer and Water Stress on the Growth and Yield of Japanese mint (Mentha arvensis L.). Bangladesh Journal of Scientific and Industrial Research 44(1): 137-145.
Solomon AM, Beemnet MK (2011). Row spacing and harvesting age affect agronomic characteristics and essential oil yield of Japanese mint (Mentha arvensis L.). Medicinal and Aromatic Plant Science and Biotechnology
5(1): 74-76.
Verma RS, Rahman L, Verma RK, Chauhan A, Yadav AK, Singh A (2010). Essential oil compostion of menthol mint (Mentha arvensis) and peppermint (Mentha piperita) cultivat at different stages of plant growth from Kumaon
region of western Himalaya. Open Access Journal of Medicinal and Aromatic Plants 1: 13-18.
Zheljazkov VD, Charles LC, Astatkie T, Wayne ME (2010). Productivity, Oil Content, and Composition of Two Spearmint Species in Mississippi. Agronomy Journal 102(1): 129-133.
Znini M, Bouklah M, Majidi L, Kharchouf S, Aouniti A, Bouyanzer A, Hammouti B, Costa J, Al-Deyab SS (2011). Chemical Composition and Inhibitory Effect of Mentha Spicata Essential Oil on the Corrosion of Steel in Molar
Hydrochloric Acid. International Journal of Electrochemical Science 6: 691-704.
Silva E, Pedro Mde A, Sogayar AC, Mohovic T, Silva CL, Janiszewski M, Cal RG, de Souza EF, Abe TP, de Andrade J, de Matos JD, Rezende E, AssunÁ„o M, Avezum A, Rocha PC, de Matos GF, Bento AM, CorrÍa AD, Vieira PC, Knobel
E; Brazilian Sepsis Epidemiological Study. Brazilian Sepsis Epidemiological Study (BASES study). Crit Care. 2004;8(4):R251-60. Comment in Linde-Zwirble WT, Angus DC. Severe sepsis epidemiology: sampling , and society. Crit Care. 2004;8(4):222-6.
Alberti C, Brun-Buisson C, Chevret S, Antonelli M,Goodman SV, Martin C, Moreno R, Ochagavia AR, Palazzo M, Werdan K, Le Gall JR; European Sepsis Study Group. Systemic inflammatory response and progression2711 
to severe sepsis in critically ill infected patient. Am J Respir Crit Care Med. 2005;171(5):461-8.
EliÈzer Silva, Luiz Dalfior Junior, HaggÈas da Silveira Fernandes,et al. Prevalence and outcomes of infections in Brazilian ICUs: a subanalysis of EPIC II study. Rev. bras. ter. intensiva vol.24 no.2 S„o Paulo Apr./June 2012
Richards MJ, Edwards JR, Culver DH, Gaynes RP. Nosocomial infections in medical intensive care units in the United States. National Nosocomial Infections Surveillance System. Crit Care Med 1999; 27: 887ñ92.
Ponce de Leon-Rosales SP, Molinar-Ramos F, DominguezCherit G, Rangel-Frausto M, Vazquez-Ramos VG. Prevalence of infections in intensive care units in Mexico: A multicentre study. Crit Care Med 2000; 28: 1316ñ21.
Avecillas J, Mazzone P, Arroliga A. A rational approach to the evaluation and treatment of the infected patient in the intensive care unit. Clin Chest Med 2003; 24: 645ñ69