Document Type: Original Article


1 MSc. student, department of fisheries, faculty of Fisheries and the Environment, Gorgan University of agricultural sciences and natural resources, Gorgan, Iran

2 Department of fisheries, faculty of Fisheries and the Environment, Gorgan University of agricultural sciences and natural resources, Gorgan, Iran


Objective: The orange-red spectrum of  the skin of  electric yellow (Labidochromis caeruleus) is  one  of  the  idealistic and  necessary  schemas sought  by  aquarists  and commercial producers. In this study, the effect of live foods meal on the skin coloration of juvenile electric yellow was examined. Methods: 270 fish with an average living body weight was 0.42 ± 0.11 g, and average total length was 3.3 ± 0.35 cm. Their sex was not taken into consideration. . The  fish  were  fed  twice  in  the  morning  and  afternoon  by  3-5 percent of the biomass for 8 weeks. The six different treatments (three replicates/treatment) used in the experiment were used. Skin color was measured in below the dorsal fin of all fish. Measurements were recorded at the end of the feeding trial using a Konica Minolta Chroma Meter CR400. Results: At the end of the trial, the carotenoid supplemented diets significantly increased the values of redness (a*), yellowness (b*), and chroma (C*), and decreased the values of lightness (L*) and hue (Hoab) on the tail, body, and head areas (p<0.05). luminosity  (L*)  and  hue (H°ab) were less in fishes fed the diets with live foods and astaxanthin than  in  fishes  fed  the  control  diet  with  no pigment sources (p<0.05). yellowness  (b*),  and  Chroma  (C*)  were  greater in fish  fed  the culex and were  less  in  fish  fed  the control diet(p<0.05). Results  show  that  live foods used in this trial  can  be  used  as  an  alternative natural  carotenoid  source  in  electric yellow  diets.


Main Subjects

Alagappan M, Vijula K, Sinha A (2004) Utilization of spirulina algae as a source of carotenoid pigment for blue gouramis (Trichogaster trichopterus, Pallas). Journal of Aquaculture Aquatic Science. 10: 1ñ11.
Barnard P.C (2011) The Royal Entomological Society Book of British Insects. Royal Entomological society of london. 383 pp.
Bengtson DA (2003) Status of marine aquaculture in relation to live prey: past, present and future. Live Feeds in Marine Aquaculture. Oxford. 1-16.
Blaustein L (1992) Larvivorous fishes fail to control mosquitoes in experimental rice plots. Hydrobiologia. 232: 219-232.
Bookhout C.G, Costlow J.D (1970) Nutritional effects of Artemia from different location on larval development of crabs. Helgolander wiss Meeresunters. 20: 435-442.
Buttle L.G, Crampton V.O, Williams P.D (2001) The effect of feed pigment type on flesh pigment deposition and color in farmed Atlantic salmon, Salmo sala L.
Aquaculture Research. 32: 103-111.
Chatzifotis S, Pavlidis M, Jimeno C.D, Vardanis A.S, Divanach P (2005) The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus). Aquaculture Research. 36: 1517-1525.
Das P, Mandal S, Bhagabati S.K, Akhtar M.S, Singh S.K (2012) Important live food organisms and their role in
aquaculture. Frontiers in Aquaculture 5, 69-86. Degani G, Yehuda Y (1996) Effects of diets on reproduction of angelfish, Pterophyllum scalare
(Cichlidae). Indian Journal of Fisheries. 43: 121-126.
De La-Noue J, Choubert G (1985) Apparent digestibility of invertebrate biomass by rainbow trout. Aquaculture. 50: 103-112.
Diler I, Hossu B, Dilek K, Emre Y, Sevgili H (2005) Effects of natural and synthetic pigments in diets on flesh coloration and growth of rainbow trout (Oncorhynchus mykiss). Aquaculture - Bamidgeh, IJA. 57: 175-184.
Ergun S, G¸roy D, Tekeºoglu H, Guroy B, «elik I, Tekinay A , Bulut M (2010) Optimum Dietary Protein Level for Blue Streak Hap (Labidochromis caeruleus). Turkish Journal of Fisheries and Aquatic Sciences. 10: 27-31.
Espinoza P, Lugo E, Valle S, Lopez D, Lopez M.M, Rivera, P , Delgado M, Garcia-Avila I (1997) Principle species of larvivorous fish, bioregulators of mosquito larvae in Nicaragua. Revista Nicaraguense de Entomologia. 40: 1-4.
Fujii R (2000) The regulation of motile activity in fish chromatophores. Pigment Cell Research. 13: 300-319.
Gouveia L, Rema P, Pereira O, Empis J (2003) Colouring ornamental fish (Cyprinus carpio and Carassius auratus) with microalgal biomass. Aquaculture Nutrition. 9: 123ñ129.
Habib, M.A.B., Ali, M.M. and Dey, N., 1992. Culture of chironomid larvae in artificial medium. Bangladesh Journal of Fisheries. 20: 63-70.
Harpaz S, Padowicz D (2007) Color enhancement in the ornamental dwarf cichlid Microgeophagus ramirezi by addition of plant carotenoids to the fish diet. Aquaculture - Bamidgeh, IJA. 59: 195-200. 

Higura-ciapara L, Felix-Valenzuela L, Goycoolea F (2006) Astaxanthin: A Review of its Chemistry and Applications. Critical Reviews in Food Science and Nutrition. 46: 185ñ 196.
James R, Sampath K, Thangarathinam R, Vasudevan I (2006) Effect of dietary levels of dietary spirulina level on growth, fertility, coloration and leucocyte count in red swordtail Xiphophorus helleri. Aquaculture - Bamidgeh,
IJA. 58: 97-104.
James R, Vasudhevan I, Sampath K (2009) Interaction of spirulina with different levels of vitamin E on growth, reproduction, and coloration in goldfish (Carassius auratus). Aquaculture - Bamidgeh, IJA. 61, 330-338.
James R, Sampath K, Thangarathinam R, Vasudevan I (2006) Effect of dietary spirulina level on growth, fertility, coloration and leucocyte count in red swordtail, Xiphophorus helleri. Aquaculture - Bamidgeh, IJA. 58: 97- 104.
James R, Vasudhevan I, Sampath K (2009) Interaction of Spirulina with different levels of vitamin E on growth, reproduction, and coloration in goldfish (Carassius auratus). Aquaculture - Bamidgeh, IJA. 61: 330-338.
Jha G.N, Sarma D, Qureshi T.A, Akhtar M.S (2012) Effect of beetroot and marigold flower meal on growth performance, carcass composition and total carotenoids of snow trout (Schizothorax richardsonii). Aquaculture -
Bamidgeh, IJA. 64: 752-759.
Kopm A, Durmaz Y (2007) The effect of synthetic and natural pigments on the color of the cichlids (Cichlasoma severum sp., Heckel 1840). Aquaculture International. 16: 117-122.
Kouakou N, Goran D.V, Choubert G (2006) Effect of varying the concentration of dietary astaxanthin on rainbow trout, Oncorhynchus mykiss, muscle
pigmentation. Journal of Applied Aquaculture. 18: 61-73.
Lee C.R, Pham M.A, Lee, S.M (2010) Effects of dietary paprika and lipid levels on growth and skin pigmentation of pale chub (Zacco platypus). Asian-Australasian Journal of Animal Sciences. 23: 724-732.
Lim C. L, Dhert P, Soregloos P (2003) Recent developments in the application of live feeds in the freshwater ornamental fish culture. Aquaculture. 227:
Matsuno T, Katsuyama M, Iwahashi M, Koike T, Okada M (1980) Intensification of color of red tilapia with lutein, rhodoxanthin and spirulina. Bulletin of
the Japanese Society of Scientific Fisheries. 46: 479-482.
Marian M.P, Christopher M.S, Selvaraj A.M, Pandian T.J (1983) Studies on predation on the mosquito Culex fatigens by Rana tigrina tadpoles. Hydrobiologia. 106: 59- 63.
Madlen M.H (2005) Culture of chironomid larvae (insecta- diptera chironomidae) under different feeding systems. Egyptian Journal of Aquatic Research. 31: 403- 418.
Mclarney W.O, Henderson S, Sherman M.M (1974) A new method for culturing Chironomus tentans larvae using burlap substrate in fertilized pools. Aquaculture. 4: 267- 276.
Nath-Jha G, Sarma D, Qureshi T.A, Akhtar M.S (2012) Effect of Marigold Flower and Beetroot Meals on Growth Performance, Carcass Composition, and Total Carotenoids of Snow Trout (Schizothorax richardsonii).
Aquaculture - Bamidgeh, IJA. 64: 752-759. 
Nelson J.S (2006) Fishes of the World. 
New M.B (1998) Global aquaculture: Current trends and challenges for the 21st century. In: Anans do Aquacultura Brasil. 98: 2-6.
Tidwell J.H, Schulmeister C.M, Coyle S (1997) Growth, survival, and Biochemical Composition of freshwater prawns Macrobracium rosenbergii fed natural food organisms under controlled conditions. Journal of the
World Aquaculture Society. 28: 123-132.
Pennak R.W (1978) Fresh water invertebrates of the United States. The Ronald Press Company, New York. 769 pp.
Scabini V, Fernandez-Palacios H, Robaina L, Kalinowski T, Izquierdo M.S (2011) Reproductive performance of gilthead seabream (Sparus aurata L., 1758) fed two combined levels of carotenoids from paprika oleoresin
and essential fatty acids. Aquaculture Nutrition. 17: 304-312.
Singaravelu G, Mahalingam S, Bharathi K.J (1997) Predatory efficiency of larvivorous fish, Gambusia affinis on the mosquito larvae of Aedes aegypti and Anopheles stephensi. Current Science. 72: 512-514.
Sahandi J (2011) Natural food production for aquaculture: cultivation and nutrition of Chironomid larvae (Insecta, Diptera). AES Bioflux. 3: 268-271.
Sugie A, Terai Y, Ota R, Okada N (2004) The evolution of genes for pigmentation in African cichlid fishes. Gene. 343: 337-346.
Yeºilayer N, Aral O, Karsli Z, Oz M, KaraÁuha A, Yagci F (2011) The effects of different carotenoid sources on skin pigmentation of goldfish (Carassius auratus). The Israeli Journal of Aquaculture ñ Bamidgeh. 63: 2011.523.
Yeºilayer N, Erdem M (2011) Effects of oleoresin paprika (Capsicum annum) and synthetic carotenoids (canthaxantin and astaxanthin) on pigmentation levels and growth in rainbow trout Oncorhynchus mykiss.
Journal of Animal and Veterinary Advances. 10: 1875-1882.
Y˝lmaz S, Ergun S (2011) Effect of red pepper (Capsicum
annuum) on pigmentation of blue streak hap
(Labidochromis caeruleus). Aquaculture ñ Bamidgeh. 63:
Y˝lmaz S, Ergun S (2013) Enhancement of Growth Performance and Pigmentation in Red Oreochromis mossambicus Associated with Dietary Intake of Astaxanthin, Paprika, or Capsicum. The Aquaculture ñ
Bamidgeh. 65: 825-832.
Yanar M, Kumlu M, Celik M, Yanar Y, Tekelioglu N (1997) Pigmentation of rainbow trout (Oncorhynchus mykiss) with carotenoids from red pepper. Aquaculture ñBamidgeh. 49: 193-198.