Document Type : Original Article

Authors

1 Graduate student, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Graduate student, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Objective: In recent years, amino acids are used in veterinary medicine for many purposes. They injected into hatching eggs to increase chick body weight and performance at hatch, but the adverse effects of amino acids have always been a major concern. There is scant information available about the pathological alterations of amino acids in the chicken embryonated eggs. The objective of this study was to investigate the pathological alterations of triaminohexanoic acid for chicken embryo. Methods: Fertile chicken eggs were divided into two equal treatment groups as follows: phosphate buffered saline-injected group and triaminohexanoic-injected group whose individuals were injected with a triaminohexanoic acid solution at a dosage of 10 mg per Kg egg-weight. Embryos were re-incubated post-treatment and allowed to develop until day 18 after which; they were examined for macroscopic lesions. Results: Results showed that embryos were normal in all treatment groups. Macroscopically, no lesions were also diagnosed in tissues. Based on macroscopic findings, it is concluded that triaminohexanoic acid at above-mentioned concentration is not toxic for the chicken embryo. Therefore, triaminohexanoic acid egg-injection can be used for increasing chick performance at hatch without any adverse effect.

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Adams HR (2001). Veterinary pharmacology and therapeutics, Iowa State University Press.
Al-Daraji H, Al-Mashadani A, Al-Hayani W, Al-Hassani A, Mirza H (2012). Effect of in ovo injection with L-arginine on productive and physiological traits of Japanese quail. S. Afr. J. Anim. Sci. 42(2): 139-145.
Bagherzadeh KF, Karimi TM, Allameh A, Shariatmadari F (2012). A novel aflatoxin-binding Bacillus probiotic: Performance, serum biochemistry, and immunological parameters in Japanese quail. Poult. Sci. 91(8): 1846- 1853.
Bhanja S, Sudhagar M, Goel A, Pandey N, Mehra M, Agarwal S, Mandal A (2014). Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickens.
Czech J. Anim. Sci. 59(9): 399-408.
Blake J, Hess J (2013). Performance of bobwhite quail fed different levels of protein and feed additive supplementation. J. App. Poult. Res. 22(2): 314-318.
Boothe DM (2011). Small animal clinical pharmacology and therapeutics, Elsevier Health Sciences.
Broekaert N, Daeseleire E, Delezie E, Vandecasteele B, De Beer T, Van Poucke C (2012). Can the use of coccidiostats in poultry breeding lead to residues in vegetables? An experimental study. J. Agric. Food Chem. 60(50): 12411-
12418.
Czech A, Ognik K, Grela E (2012). Efficacy of a mixture of synthetic antioxidant and protein-xanthophyll alfalfa concentrate in turkey hens feeding. Archive Fur Geflugelkunde 76(2): 105-112.
Devreese M, Girgis GN, Tran S-T, De Baere S, De Backer P, Croubels S, Smith Fusarium mycotoxins and glucomannan in turkey poults
based on specific and non-specific parameters. Food Chem. Toxicol. 63(69-75.
Fernstrom JD (2013). Large neutral amino acids: dietary effects on brain neurochemistry and function. Amino Acids 45(3): 419-430.
Fulton J (2012). Genomic selection for poultry breeding. Animal Frontiers 2(1): 30-36.
Gaafar KM, Selim SA (2012). Effect of in-ovo administration with two levels of amino acids mixture on the performance of Muscovy ducks. Emirates J. Food Agri. 25(1): doi: 10.9755/ejfa. v9725i9751. 9666.
Golan DE, Tashjian AH, Armstrong EJ (2011). Principles of pharmacology: the pathophysiologic basis of drug therapy, Lippincott Williams & Wilkins.
Gracia MI, S·nchez J, Casabuena O, Wiemann M, Weber GM (2013). Effect of an Eubiotic feed additive on broiler performance in energy reduced diets. 19th European Symposium On Poultry Nutrition.
Hamburger V (1942). A manual of experimental embryology, University of Chicago Press Chicago.
Huff G, Huff W, Jalukar S, Oppy J, Rath N, Packialakshmi B (2013). The effects of yeast feed supplementation on turkey performance and pathogen colonization in a transport stress/Escherichia coli challenge. Poult. Sci.
92(3): 655-662.
Jacobsen ID, Grofle K, Hube B (2012). Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi. Host-Fungus Interactions, Springer: 487-496.
Khan TA, Khan MN, Hasan R, Fatima H, Kousar E (2013). Effects of Nigella sativa (Black Seed) on Serum Levels of Urea and Uric Acid in Acetaminophen Induced Hepatotoxicity of Commercial Layer Chickens. J. Worldís
Poult. Res. 3(4):
Khosravifar O, Ebrahimnezhad Y, Maheri-Sis N, Nobar R, Ghiasi-Galekandi J (2014). Effect of some medicinal plants as feed additive on total coliform count of ileum in Japanese quails (Coturnix coturnix japonica). Int. J. Biosci.
(IJB) 4(2): 211-220.
KingíOri A (2011). Review of the factors that influence egg fertility and hatchability in poultry. Int. J. Poult. Sci 10(6): 483-493.
Kita K, R Ito K, Sugahara M, Kobayashi M, Makino R, Takahashi N, Nakahara H, Takahashi K, Nishimukai M (2014). Effect of In Ovo Administration of BranchedChain Amino Acids on Embryo Growth and Hatching Time of Chickens. J. Poult. Sci. 11(8): 583-593.
Kogut MH, Genovese KJ, He H, Swaggerty CL, Jiang Y (2013). Modulation of Chicken Intestinal Immune Gene Expression by Small Cationic Peptides as Feed Additives during the First Week Posthatch. Clin. Vaccine Immunol.
20(9): 1440-1448.
Li X, Rezaei R, Li P, Wu G (2011). Composition of amino acids in feed ingredients for animal diets. Amino Acids 40(4): 1159-1168.
Li X, Sui W, Yan H, Jiang Q, Wang X (2014). The in ovo administration of l-trans pyrrolidine-2, 4-dicarboxylic acid regulates small intestinal growth in chicks. Int. j. anim. Biosci. 8(10): 1677-1683.
Lim T-H, Lee D-H, Lee Y-N, Park J-K, Youn H-N, Kim M-S, Lee H-J, Yang S-Y, Cho Y-W, Lee J-B (2011). Efficacy of bacteriophage therapy on horizontal transmission of Salmonella Gallinarum on commercial layer chickens.
Avian Dis. 55(3): 435-438.
McCapes RH, Yamamoto R, Ortmayer HB, Scott WF (1975). Injecting antibiotics into turkey hatching eggs to eliminate Mycoplasma meleagridis infection. Avian Dis 19(3): 506-514.
Meijer AJ, Lorin S, Blommaart EF, Codogno P (2014). Regulation of autophagy by amino acids and MTORdependent signal transduction. Amino Acids 1-27.
Mosallanejad S, Tavakkoli H, Derakhshanfar A, S S (2014a). Efficiency of the injection of trimethoprim/sulfamethoxazole solution on game bird
embryonated-egg during the late stage of development. Int. J. Adv. Biol. Biom. Res. 2(5): 1553-1561.
Mosallanejad S, Tavakkoli H, Derakhshanfar A, S S (2014b). An experimental study of the systemic alteration of nitroimidazoles in the middle stage of
embryonic development. Int. J. Adv. Biol. Biom. Res. 2(5):1468-1474.
Mountzouris K, Paraskevas V, Tsirtsikos P, Palamidi I, Steiner T, Schatzmayr G, Fegeros K (2011). Assessment of a phytogenic feed additive effect on broiler growth performance, nutrient digestibility and caecal microflora composition. Anim. Feed Sci. Technol. 168(3): 223-231.
Murakami A, Rodrigueiro R, Santos T, Ospina-Rojas I, Rademacher M (2014). Effects of dietary supplementation of meat-type quail breeders with
guanidinoacetic acid on their reproductive parameters and progeny performance. Poult. Sci. 93(9): 2237-2244.
Nascimento ER, Pereira V, Nascimento M, Barreto M (2005). Avian mycoplasmosis update. Revista Brasileira de CiÍncia AvÌcola 7(1): 1-9.
Oakley BB, Buhr RJ, Ritz CW, Kiepper BH, Berrang ME, Seal BS, Cox NA (2014). Successional changes in the chicken cecal microbiome during 42 days of growth are independent of organic acid feed additives. BMC Vet. Res.
10(1): 282.
Ognik K, Krauze M (2012). Dietary supplementation of mannanoligosaccharides to turkey hens on their growth performance and antioxidant status in the blood. S. Afr. J. Anim. Sci. 42(4): 379-388.
Rezaei R, Wang W, Wu Z, Dai Z, Wang J, Wu G (2013). Biochemical and physiological bases for utilization of dietary amino acids by young pigs. J Anim Sci Biotech 4(7):
Rutherfurd S, Chung T, Thomas D, Zou M, Moughan P (2012). Effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus cornsoybean meal diet for broilers. Poult. Sci. 91(5): 1118- 1127.
Schwarz A, Gauly M, Abel H, Daº G, Humburg J, Rohn K, Breves G, Rautenschlein S (2011). Immunopathogenesis of Ascaridia galli infection in layer chicken. Dev. Comp. Immunol. 35(7): 774-784.
Shafey T, Mahmoud A, Alsobayel A, Abouheif M (2014). Effects of in ovo administration of amino acids on hatchability and performance of meat chickens. S. Afr. J. Anim. Sci. 44(2): 123-130.
Surai P, Fisinin V (2012). The modern anti-stress technologies in poultry: from antioxidants to vitagenes. Selískokhozyaistvennaya Biologiya. Agricul. Biol. 4: 3-12.
Swain B, Naik P, Chakurkar E, Singh N (2014). Effect of biovet on performance, egg quality characteristics and hatchability in quail breeders. Indian Journal of Animal Research 48(3): 281-285.
Tavakkoli H, Derakhshanfar A, Noori Gooshki S (2014a). The effect of florfenicol egg-injection on embryonated chicken egg. International Journal of Advanced Biological and Biomedical Research. 2(2): 496-503.
Tavakkoli H, Derakhshanfar A, Salandari S (2104). Investigation on the using of linco-spectin solution for in ovo administration in chicken embryo. International Journal of Advanced Biological and Biomedical Research. 2(1): 110-116.
Tavakkoli H, Noori Gooshki S (2014b). The Effect of Doxycycline on the Viability of the Quail Embryo During Incubation Period. International Journal of Advanced Biological and Biomedical Research. 2(8): 2390-2394.
Tripathi K (2013). Essentials of medical pharmacology, JP Medical Ltd.
Wu G (2013). Functional amino acids in nutrition and
health. Amino Acids 45(3): 407-411.
Wu G, Wu Z, Dai Z, Yang Y, Wang W, Liu C, Wang B, Wang J, Yin Y (2013). Dietary requirements of ìnutritionally non-essential amino acidsî by animals and humans. Amino Acids 44(4): 1107-1113.
Zhai W, Peebles E, Zumwalt C, Mejia L, Corzo A (2013). Effects of dietary amino acid density regimens on growth performance and meat yield of Cobb× Cobb 700 broilers.
J. App. Poult. Res. 22(3): 447-460. Zhou Y, Yoon J (2012). Recent progress in fluorescent and colorimetric chemosensors for detection of amino acids. Chem. Soc. Rev. 41(1): 52-67.