Document Type: Original Article
Authors
- Beemnet Mengesha Kassahun 1
- Tigist German 1
- Zewdinesh Damtew Zigene 1
- Samuel Tilahun 2
- Aynalem Gebere 1
- Seferu Taddese 3
- Desta Fikadu 1
- Beriso Miesso 3
1 Department of Horticulture, Ethiopian Institute of Agricultural Research, Wondo Genet Agricultural Research Center, P. O Box, 198 Shashemene, Ethiopia
2 Department of Plant Science, Ethiopian Institute of Agricultural Research, Holleta Agricultural Research Center, Ethiopia
3 Department of Chemistry, Ethiopian Institute of Agricultural Research, Wondo Genet Agricultural Research Center, P. O Box, 198 Shashemene, Ethiopia
Abstract
This experiment was conducted with the objective of evaluating the performance sage (Salvia officinalis L.) in different agroecology of Ethiopia for morpho-agronomic and chemical traits at Wondo genet, Hawassa, Qoqa and Holleta for two years from 2011/2012 to 2012/2013 cropping seasons. Data on plant height, branch number/plant, fresh leaf weight/plant, fresh stem weight/plant, above ground biomass/plant, fresh leaf weight/ha, essential oil (EO) content and EO yield/ha were collected from four locations arranged in randomized complete block design with four replications. Growing location demonstrated a significant influence (P<0.05) on the performance of all the parameters considered. With increasing year, the performance of Sage was significantly affected (P<0.05) only on three of the nine traits. The interaction between location and yea was significant for seven of the nine traits. The overall average value of plant height, branch number/plant, leaf number/plant, fresh leaf weight/plant, fresh stem weight/plant, above ground biomass/plant, fresh leaf weight/ha EO content and EO yield/ha of sage across the tested location and years ranged from 47.7-66.9 cm, 70.13-262.07, 619.7-4046.2, 159.13-506.31 g, 63.77-279.93 g, 221.6-743.8 g, 2772-14064 kg, 1.11-1.70% and 30.67-240.03 kg, respectively. The maximum for all the characters were recorded at Hawassa and Wondo Genet except for branch number which was found tall at Holleta. Most of the characters are statistically the same over the testing years; however, plant height, branch number and leaf number/plant demonstrated a respective percent increase value of 21.3%, 50.19% and 71.98% during the first testing year.
Keywords
Main Subjects
Aflatuni, A., 2005. The yield and essential oil content of mint (Mentha spp.) in Northern Ostrobothnia. Phd thesis, University of Oulu, Finland.
Agha Q., Ahmad, S., Islam, M., Gill, A., Athar, M., 2011. Growth and production potential of three exotic herbs in highlands of Balochistan, Pakistan. J. Med. Plant. Res., 5(4), 543-548.
Alizadeh, A., Khoshkhui, M., Javidnia, K., Firuzi, O.R., Tafazoli, E., Khalighi, A., 2010. Effects of fertilizer on yield, essential oil composition, total phenolic content and antioxidant activity in Satureja hortensis L. (Lamiaceae)
cultivated in Iran. J. Med. Plant. Res., 4, 33-40.
Al-Tawaha, A., Al-Karaki, G., Massadeh, A., 2013. Antioxidant activity, total phenols and variation of chemical composition from essential oil in sage (Salvia officinalis L.) grown under protected soilless condition and open
field conditions. Adv. Environ. Biol., 7(5), 894-901.
Andargachew, G.A., 2007. Indigenous knowledge and genetic diversity of cultivated Amochi (Arisaema schimperianum Schott). PhD thesis, Department of Environmental and Plant Sciences, Norwegian university
of life sciences, Norway, 1-49.
Ardakani, M.R., Abbaszadeh, B., Haghighi, M.L., 2014. The effect of drought stress on three clary sage (Salvia sclarea L.) populations from different habitats. J. Biodiv. Env. Sci., 5(4), 133-142.
Aruoma, O.I., 1998. Free radicals, oxidative stress, and antioxidants in human health and disease. J. Am. Oil. Chem. Soc., 75, 199 –212.
Aziz, E.E., Sabry, R.M., Ahmed, S.S., 2013. Plant growth and essential oil production of Sage (Salvia officinalis L.) and curly-leafed Parsley (Petroselinum crispum ssp. crispum L.) Cultivated under Salt Stress Conditions. World. Appl. Sci. J., 28(6), 785-796.
Baricevic, D., Sosa, S., Loggia, R.D., Tubaro, A., Simonovska, B., Krasna, A., Zupancic, A., 2001. Topical antiinflammatory activity of Salvia officinalis L. Leaves: the relevance of ursolic acid. J. Ethnopharm., 75, 125-132.
Beemnet, M., Getinet, A., 2010. Variability in Ethiopian coriander for agronomic and quality traits. Afr. Crop. Sci. J., 18(2), 43-49.
Beemnet, M.K., Solomon, A., Zinash, T.A., Haileslassie, G.K., Beniyam, Y., Gizachew, A., Bekri, M., Wossen, K.M., Texeria, D.S., 2011. Performance of lemongrass (Cymbopogon citratus L. (DC) Stapf) for agronomic and
chemical traits in different agro-ecologies of Ethiopia. Med. Aromat. Plant. Sci. Biotechnol., 5(2), 133-138.
Beemnet, M.K., Zewdinesh, D.Z., Wossen, K., Hailesilasie, G., 2012. Performance of Stevia (Stevia rebaudiana Bertoni) for morphological and economic traits under different ecologies of Ethiopia. J. Agr. Dev., 2(2), 54-67.
Beemnet, M.K., Desta Egeta, F., Wondu, B.Y., Tewodros, L., 2014c. Variability in lemongrass (Cymbopogon citratus (DC) Stapf.) genotypes for agronomic and chemical characters in Ethiopia. Int. J. Green. Herbal. Chem., 3(4),
1482-1489.
Beemnet, M.K., Desta, F.E., Tewodros, L., Wondu, B.Y., Seferu, T., 2014b. Variability in agronomic and chemical
characteristics of Spearmint (Mentha spicata L.) genotypes in Ethiopia. Int. J. Advanc. Biol. Biomed. Res.,
2(10), 2704-2711.
Beemnet, M.K., Samuel, T., Zewdinesh, D.Z., Zinash, T., Mihret, M., Bekri, M., 2014a. Morpho-agronomic characteristics, essential oil content and essential oil yield of Oregano (Origanum vulgare L.) in Ethiopia. Schol. J. Agr. Sci., 4(12), 565-571.
Beemnet, M.K., Zinash, T., Zewdinesh, D.Z., Wondu Bekele, Bekri Melka, Basazenew Degu, Tigist German, Desta Fekadu, Hassen Nurhusain, 2015. Evaluation of American and German chamomiles for agronomic and
chemical traits in Ethiopia. Res. J. Agr. Env. Manag., 4(3), 134-140.
Beemnet, M.K., Wondu, B., Solomon, A.M., 2013. Performance of lemon Verbena (Aloysia triphylla L.) for morphological, economic and chemical traits in Ethiopia. Am. Eur. J. Agr. Env. Sci., 13(11), 1576-1581.
Belay, B., 2007. Assessment of crop growth and artemisinin content of the medicinal plant Artemisia annua Anamed in the Gamo highlands of SW Ethiopia. MSc. Thesis, University of Hohenheim, Sttutgart, Germany.
Boszormenyi, A, Hethelyi, E., Farakas, A., Horrath, G., Papp, N., Lemberkorics, E., Szoke, E., 2009. Chemical and genetic relationships among Sage (Salvia officinalis L.) cultivars and judean Sage (Salvia judaica Boiss.). J. Agr.
Food. Chem., 57, 4663-4667.
Chalchat, J.C., Michet, A., Pasquier, B., 1998. Study of clones of Salvia officinalis L. yields and chemical composition of essential oil. Flav. Frag. J., 13, 68-70.
Cuvelier, M.E., Berset, C., Richard, H., 1994. Antioxidant constituents in sage (Salvia officinalis). Khimiya Prirodnykh Soedineii., 5, 686-687.
Delamare, A.P.L., Moschen-Pistorello, I.T., Artico, L., Atti-Serafini, L., Echeverrigaray, S., 2007. Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food. Chem., 100, 603-608.
EIAR (Ethiopian Institute of Agricultural Research), 2009. MOU agreement between Ariti Helblal plc, Fana Aromatic and Medicinal plants growers’ private limited association and Wondo Genet Agricultural Research Center for
production, processing and commercialization of Aromatic and Medicinal Plants. Ethiopian. Instit. Agr. Res. (EIAR), Addis Ababa, Ethiopia, 10.
Eidi, M., Zarrindast, M.R., Eidi, A., Oryan, S., 2003. Effects of histamine and cholinergic systems on memory retention of passive avoidance learning in rats. Eur. J. Pharmacol., 465, 91-6.
Fahmy, I.R., Saad, S.N., Ahmed, Z.F., 1955. Effect of environment on yield and quality of the oil of Mentha piperita Grown in Egypt. Chemical Abstract, 51, 8379-80.
Farag, R.S., Badei, A., Hewedi, F.M., El-Baroty, G.S.A., 1989. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. J. Am. Oil. Chem. Soc., 66, 792-9.
Fehr, W.R., 1991. Principles of cultivar development theory and technique, Iowa State University, USA, 247-260.
Gomez, K.A., Gomez, A.A., 1984. Statistical procedures for agricultural research (2nd Edn), John Wiley and Sons, New York, 680.
Grzegorcazy, K.I., Wysokinska, H., 2008. Liquid shoot culture of Salvia officinalis L. of micro propagation and production of antioxidant compounds; Effect of Tria Contanol. Acta Societatis Botanicorum Polonie, 77(2), 99-
104.
Guenther, E., 1972. The essential oils: history-origin in plants production-analysis (Vol I), Robert, E. Kriger Publishing Co., Malabar, Florida, 427.
Gulcin, I., 2005. The antioxidant and radical scavenging activities of black pepper (Pipernigrum) seeds. Inter. J. Food. Sci. Nut., 56, 491-499.
Hohmann, J., Zupko, I., Redei, D., 1999. Protective effects of the aerial parts of Salvia officinalis, Melissa officinalis and Lavandula angustifolia and their constituents against enzyme-dependent and enzyme independent lipid
peroxidation. Planta. Med; 65, 576-8.
IRRI (International Rice Research Institute), 1996. Plant adaptation and crop improvement, CAB International, Manila, Philippines, 3-5.
Kamatou, G.P.P., Makunga, N.P., Ramogola, W.P.N., Viljoen, A.M., 2008. South African Salvia species: a review of biological activities and phytochemistry. J. Ethnopharm., 119, 664-672.
Kebebew, A., 2003. Phenotypic and molecular diversity in the Ethiopian cereal, Tef (Eragrosits tef (Zucc) Trotter): implications on conservation and breeding. Phd thesis, Department of plant science, Swidish university of Agricultural Sciences, Alnarp, 7-18.
Kelen, M., Tepe, B., 2008. Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Biores. Techno., 99, 4096-4104.
Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., Hilpert, K.F., 2002. Bioactive compounds in foods: Their role in the prevention of cardiovascular disease and cancer. Am. J. Med., 113(9B), 71-88.
Lakusic, B.S., Ristic, M.S., Slavkovska, V.N., Stojanovic, D.L., Lakusic, D.V., 2013. Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. Botanica Serbica, 37(2), 127-
139.
Lamaison, J.L., Petitjean-Freytet, C., Carnat, A., 1990. Teneurs en acide rosmarinique, en derives hydroxycinnamiques totaux et activité antioxydante chez les Apiacées, les Boraginaceé et les Lamiacées médicinales. Annu Pharm Fr, 48, 103-108.
Loizzo, M.R., Saab, A.M., Tundis, R., Menichini, F., Bonesi, M., Piccolo, V., Statti, G.A., de Cindio, B., Houghton, P.J., Menichini, F., 2008. In vitro inhibitory activities of plants used in Lebanon traditional medicine against
angiotensin converting enzyme (ACE) and digestive enzymes related to diabetes. J. Ethnopharm., 119, 109-116.
Loizzo, M.R., Tundis, R., Menichini, F., Saab, A.M., Statti, G.A., Menichini, F., 2007. Cytotoxic activity of essential oils from Labiatae and Lauraceae families against in vitro humantumor models. Anticancer. Res., 27, 3293-3299.
Lu, Y., Foo, Y.L., 2001. Antioxidant activities of polyphenols from Sage (Salvia officinalis). Food. Chem., 75, 197-202.
Miller, A.B., 1976. Shaker herbs: a history and a compendium. General Publishing Company Limited: New York, 90.
Mirjalili, M.H., Salehi, P., Sonboli, A., Vala, M.M., 2006. Essential oil variation of Salvia officinalis aerial parts during
its phenological cycle. Chemistry of Natural Compounds, 42, 19-23.
Nees, A.R., Powles, J.W., 1997. Fruit and vegetables and cardiovascular disease: a review. Int. J. Epidemiol., 26, 1-
13.
NMSA (National Metrology Service Agency), 1996. Climatic and Agro climatic resources of Ethiopia (Vol I). National Metrology Service Agency of Ethiopia, Addis Ababa, 137.
Perry, N.B., Anderson, R.E., Brennan, N.J., Douglas, M.H., Heanney, A.J., McGimpsey, J.A., 1999. Essential oils from dalmatian sage (Salvia officinalis L.): Variations among individuals, plant parts, seasons and sites. J. Agr. Food.
Chem., 47, 2048-2054.
Psomiadou, E., Tsimidou, M., 2002. Stability of virgin olive oil autoxidation studies. J. Agric. Food. Chem., 50, 716-721.
Putievsky, E., Ravid, E., Dudai, N., 1986. The influence of season and harvest frequency on essential oil and herbal yields from a pure clone of sage (Salvia officinalis) grown under cultivated conditions. J. Natu. Prod., 49, 326-
329.
Raina, A.P., Negi, K.S., Dutta, M., 2013. Variability in essential oil composition of sage (Salvia officinalis L.) grown under North Western Himalayan Region of India. J. Med. Plant. Res., 7(11), 683-688.
Santos-Gomes, P.C., Fernandes-Ferreira, M., 2001. Organ- and season-dependent variation in the essential oil composition of Salvia officinalis L. cultivated at two different sites. J, Agr. Food. Chem., 49, 2908-2916.
SAS (Statistical Analysis System), 2002. SAS/STAT. Guide Version 9, SAS, Institute Inc. Raleigh, North Carolina, USA.
Snedecor G.W., W.G. Cochran, (1990). Statistical Methods. 11th Ed. Iowa State College Press. Ames, Iowa, U.S., 369-375.
Steinmetz, K.A., Potter, J.D., 1996. Vegetables, fruit, and cancer prevention: a review. J. Am. Diet. Assoc., 96, 1027-1039.
Tildesley, N.T.J., Kennedy, D.O., Perry, E.K., Ballard, C.G., Wesnes, K.A., Scholey, A.B., 2005. Positive modulation of mood and cognitive performance following administration of acute doses of Salvia lavandulaefolia essential
oil to healthy young volunteers. Physiol. Behav., 83, 699-709.
Tildesley, N.T.J., Kennedy, D.O., Perry, E.K., Ballard, C., Savelev, S., Wesnes, K.A., Scholey, A.B., 2003. Salvia lavandulaefolia (Spanish sage) enhances memory in healthy young volunteers. Pharmacol. Biochem. Behav.,
75, 669-674.
Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D., 1998. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J. Agri. Food. Chem., 46, 4113- 4117.
Zheng, W., Wang, S.Y., 2001. Antioxidant activity and phenolic compounds in selectedherbs. J. Agri. Food. Chem., 49, 5165-5170.
