Document Type : Original Article

Author

Department of Animal Nutrition, Sumitra Research Farm, Gujarat, India

10.33945/SAMI/IJABBR.2019.4.8

Abstract

The objective of this study was to evaluate the heamato-biochemical, relative organ weight and bacteria count of broiler chicken given different levels of Luffa aegyptiaca leaf extract (LUF). A total of 250 day old Ross 308 broiler chicks of mixed sex were divided into five treatments, each group was further divided into five replicates each of ten (10) birds in a completely randomized design. Birds in treatment A were given 1.20g/litre of Neomycin in water, treatment B, C, D and E were given 5, 10, 15 and 20 ml/litre of LUF. Clean feed and water was given ad libitum and the experiment lasted for 3 weeks. The data obtained was used to evaluate the haematological parameters (PCV, RBC, Hb, WBC, MCV, MCH and MCHC), serum biochemical indices (Albumin, globulin, calcium, phosphorus, SGPT and SGOT), organ weight (liver, kidney, spleen, heart, lungs, pancreas and small intestine) and bacteria count (E.coli and Lactobacillus). Result obtained revealed that there was no significant differences (p>0.05) in the haematological, relative organ weight, serum biochemical parameters and E.coli count obtained. However, there was a significant difference (p

Keywords

Adili, N, Melizi, M. (2013). The effect of age, sex and altitude on the morphometry of red blood cells in small ruminants. J. Animal. Sci. Adv., 3(1):27-32.
Akintomide, AA, Joseph, OG, Onibi, GE. (2016). Hematological and serum biochemistry of cockerels fed diets containing Neem leaf meal. Appl. Trop. Agr., 23:12-16.
Albokhadaim, I. (2012). Haematological and some biochemical values of indigenous chickens in Al-Ahsa, Saudi Arabia during summer season. Asian J. Poult. Sci., 6(4):138-145.
AOAC, (2000). Association of Official Analytical Chemist Official Method of Analysis 15th Edition Washington, D.C. pages 70-88.
Bird, A, Croom, W, McBride, B. (2002). Dietary management of the intestinal microbiota using probiotics and prebiotics in human and animals. Proc. Aust. Poult. Sci. Sym., 14:35-43.
Bolu, SAO, Sola-Ojo, FE, Olorunsanya, OA, Idris, K. (2009). Effect of graded levels of Pawpaw Seed on the Performance, haematology and Serum biochemistry and Carcass evaluation of Broiler chickens. Asian Network for Scientific Information, Int. J. Poultry Sci., 8(9):905-909.
Doumas, BT, Briggs, HG. (1972). Serum Albumen Bromocresol Green Binding Standard Methods. Clin. Chem., 18(7):175-179.
Farmer, JJ. (2003). Enterobacteriaceae: Introduction and Identification, in Manual of Clinical Microbiology, 8th ed., Murray, P.R. et al., Eds., ASM Press, Washington, DC, chap. 41.
Hanan E. Al-Mashhadani (2014). Effect of different levels of turmeric supplementation on broiler performance, carcass characteristics and bacteria count of broiler chicken. Egyptian Poult. Sci. J., 35(1):25-39.
Harbone, JB. (1973). A guide to modern techniques to plant analysis. Chapman and hall, New York, USA 2nd Edition.
Ibrahim, NDG, Abdulrahman, EM, Ibrahim G. (2001). Elemental analysis of the leaves of Vernonia amydalina and its biological evaluation in rats. Nigerian J. Natural Product. Med., 5:13-17.
Igwe, CU, Onyeze, GOC, Onwuliri, VA, Osuagwu, CG, Ojiako, AO. (2010). Evaluation of the chemical composition of the leaf of Spondias mombin Linn from Nigeria. Aus. J. Basic Appl. Sci., 4(5):706-710.
Islam, MS, Lucky, NS, Islam, MR, Ahad, A, BR, Das, BR, Rahman, MM, Siddui, MSI. (2004). Hematological parameters of Fayoumi Assil and local chicken reared in sylhet region of Bangladesh. Int. J. Poult. Sci., 3(2):144-147.
Jain, NC. (1986). Schalms veterinary hematology 4th edition Lea and Febiger, Philadelphia.
Jong, H, Ji, WL, Kyoung, SK, Ju-Sung, K, Sang, NH, Chang, YY, Ju, KL, Yong, SK, Myong, JK. (2010). Antioxidant and antimicrobial activities of extract from a medicinal plant. Sea Buckthorn. J. Kor. Soc. Appl. Bio. Chem., 53:33–38.
Mabhiza, D, Chitemerere, T, Mukanganyama, S. (2016). Antibacterial properties of alkaloids extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. Int. J. Med. Chem., 2016:1-7. http://dx.doi.org/10.1155/2016/6304163
Madhusadhan, KT, Ranesh, HP, Ogawa, T, Sasoka, K, Singh, N. (1986). Detoxification of commercial linseed meal for use in broiler ration. Poultry Sci., 65:164-171.
Middleton, E, Kandaswani, C, Theoharides, TC. (2000). The effect of plant flavonoids on mammalian cells, implication for inflammation, heart disease and cancer. Pharmacol., 52:673-751.
Minka, NS, Ayo, JO. (2007). Physiological responses of transported goat treated with Ascorbic acid during hot dry season. Anim. Sci. J., 78(2):164-172.
Murray, RK, Granner, DK, Mayes, PA, Rodwell, VW. (2000). Harpers Biochemistry, 25th edition, McGraw – Hill, Health Profession Division, USA, 225.
Naderi, M, Akbari, MR, Asadi-Khoshoei E, Khaksar K, Khajali F. (2014). Effect of dietary inclusion of Turmeric and curcurmon powders on the performance, organ relative weight and immune system parameters of broiler chicken. Poult. Sci. J., 2:153-163.
NRC, National Research Council (1994). Nutrient Requirement for Poultry (9th red) National Academy Press. Washington D. C, USA.
Obi, IU. (2002). Statistical methods for detecting differences between treatment means and research methodology issue in laboratory and field experiments. A.P. Company Limited., Nigeria.
Okaka, JC, Enoch, NJ, Okaka NC. (1992). Human Nutrition: An integrated approach ESUT Publications, Enugu, Pp: 57-58.
Panda, AK, Rao, SVR, Raju, MVLN, Sharma, SR. (2006). Dietary supplementation of Lactobacillus sporogenes on performance and serum biochemico-lipid profile of broiler chickens. J. Poult. Sci., 43(3):235-240.
Scott, HM. (1965). Measurement of Albino Acid content of fish meal protein by chick growth assay, P.Sc., pp: 395-65.
Shakeri, A, Hazeri, N, Vlizadeh, J, Ghasemi, A, Tavallaei, F. (2012). Phytochemical screening, antimicrobial and antioxidant activity of Anabasis aphylla L. extracts. Kregujevac J. Sci., 34:71-78.
Snedecor, GW, Cochran, WG. (1978). Statistical method 6th edition Lowa State University press, Amen Lowa.
Snedecor, GW, Cochran, GW. (1978). Statistical methods 6th edition, the IOWA University Press Ames, IOWA, USA. 258-299.
Soetan, KO, Olaiya, CO, Oyewole, OE. (2010). The importance of mineral elements for humans, domestic animals and plants: A review. African J. Food Sci., 4(5):200-222.
Sofowora, A. (1993). Medicinal plants and Traditional Medicine in Africa. Spectrum Books Limited Ibadan. Nigeria. P: 289.
Talebi, A, Asri-Rezaei, S, Rozeh-Chai, R, Sahraei, R. (2005). Comparative studies on haematological values of broiler strains. Int. J. Poult. Sci., 4(8):573-579.
Tiwari, DP, Jyoti, P. (2008). Antibiotic Probiotic and Prebiotic as feed additives: Health benefits and safety. Food Quality Assurance: a concentrated approach p: 111-130.
Valerie, AS, Ibi, A, Toge, C, Amuzie UF, Ftepti BJ, Raji, B. (2017). Antimicrobial and physicochemical evaluation of L. aegyptiaca extracts. Biochem. Molecular Biology, 2(6):80-85.
Vikas, K, Sinha, AK, Makka, HPS, Becker K. (2009). Dietary roles of phytate and phytase in human nutrition: A review. Food Chem., 120:945–959. www.elsevier.com/locate/foodchem
Watkins, BA, Kratzer, FH. (1983). Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks. Poult. Sci., 62(10):2088-2094.
Wojdylo, A, Oszmianski, J, Czemerys, R. (2007): Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem., 105:940-949.