Document Type: Original Article

Authors

Department of Biological sciences, Faculty of Science, University of Kurdistan, Sanandaj, IRAN

10.33945/SAMI/IJABBR.2020.2.6

Abstract

Background:
α-Glucosidase Inhibition can significantly prevent glucose uptake after meal, and helps in controlling of some adverse effects in diabetics. So determination of α-Glucosidase inhibitory effect and antioxidant activity of the Haplophyllum acutifolium and Ferula haussknechtii aerial organs was the aim of this study.
Methods:
Inhibitory effect of hexane extracts from different organs was investigated in several concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of hexane extracts of various organs was also measured using DPPH and iron reduction tests.
Results;
The highest inhibitory activity of F.haussknechtii was observed at the 0.1 g/ml concentration of flower extract (100% inhibition and IC50= 0.1 μg /ml) and the most inhibitory activity of H.acutifolium, was related to the 1 g/ml concentration of flower extract (100% inhibition and IC50 = 10 μg /ml) and leaf extract (100% inhibition and IC50 = 60 μg /ml). Extract of H.acutifolium flower and leaves showed Non-competitive inhibition pattern and F.haussknechtii flower showed mixed (Competitive -Non-Competitive) inhibitory pattern at 0.001 g/ml and exhibit uncompetitive inhibitory pattern at the 0.1 g/ml. The results of antioxidant potential showed EC50 for F.haussknechtii flower and H.acutifolium leaves equalled 2.37 and 0.96 mg/ml, respectively.
Conclusions:
The hexane extract of the F.haussknechtii flower, and H.acutifolium flower and leaf organ have a significant inhibitory effect on the activity of α-Glucosidase, DPPH free radical scavenging activities and reducing power. So they are good resources for extraction of medicinal compounds to control blood level of glucose after meal, in diabetic patients.

Keywords

Ahmed, D, Kumar, V, Sharma, M, Verma, A. (2014). Target guided isolation, in-vitro antidiabetic, antioxidant activity and molecular docking studies of some flavonoids from Albizzia Lebbeck Benth. bark. BMC Complemen. Alternat. Med., 14:155-168.

Alam, MS, Kaur, G, Jabbar, Z, Javed, K, Athar, M. (2007). Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. Food Chem. Toxicol., 45:910-920.

Bachhawat, J, Shihabudeen, M, Thirumurugan, K. (2011). Screening of fifteen Indian ayurvedic plants for alpha-glucosidase inhibitory activity and enzyme kinetics. Int. J. Pharm. Pharm. Sci., 3:267-274.

Basak, SS, Candan, F. (2013). Effect of Laurus nobilis L. essential oil and its main components on α-glucosidase and reactive oxygen species scavenging activity. Iran. J. pharma. Res. (IJPR), 12:367-379.

Becerra-Jiménez, J, Andrade-Cetto, A. (2012). Effect of Opuntia streptacantha Lem. on alpha-glucosidase activity. J. ethnopharmacol., 139:493-496.

Bonnefont-Rousselot, D, Bastard, J, Jaudon, M, Delattre, J. (2000). Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes metabolism, 26:163-177.

Bovicelli, P. (2007). Radical‐scavenging polyphenols: new strategies for their synthesis. J. Pharm. Pharmacol., 59:1703-1710.

Calabrese, V, Mancuso, C, Sapienza, M, Puleo, E, Calafato, S, Cornelius, C, Finocchiaro, M, Mangiameli, A, Di Mauro, M, Stella, AMG. (2007). Oxidative stress and cellular stress response in diabetic nephropathy. Cell Stress Chaperones, 12:299-306.

Chang, CC, Yang, MH, Wen, HM, Chern, JC. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 10:178-182.

Chung, YC, Chien, CT, Teng, KY, Chou, ST. (2006). Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & zucc. Food Chem., 97:418-425.

Fatehi-Hassanabad, Z, Chan, CB, Furman, BL. (2010). Reactive oxygen species and endothelial function in diabetes. Eur. J. Pharmacol., 636:8-17.

Fu, R, Zhang, Y, Guo, Y, Chen, F. (2014). Antioxidant and tyrosinase inhibition activities of the ethanol-insoluble fraction of water extract of Sapium sebiferum (L.) Roxb. leaves. South Afr. J. Botany, 93:98-104.

Giacco, F, Brownlee, M. (2010). Oxidative stress and diabetic complications. Circulat. Res., 107:1058-1070.

Hinneburg, I, Dorman, HD, Hiltunen, R. (2006). Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem., 97:122-129.

Hünkar, T, Aktan, F, Ceylan, A, Karasu, C. (2002). Effects of cod liver oil on tissue antioxidant pathways in normal and streptozotocin‐diabetic rats. Cell Biochem. Funct., 20:297-302.

Koo, I, Kim, S, Zhang, X. (2013). Comparative analysis of mass spectral matching-based compound identification in gas chromatography–mass spectrometry. J. chromatog. A, 1298:132-138.

Kurzyna-Młynik, R, Oskolski, AA, Downie, SR, Kopacz, R, Wojewódzka, A, Spalik, K. (2008). Plant System. Evolut., 274:47.

Lawag, IL, Aguinaldo, AM, Naheed, S, Mosihuzzaman, M. (2012). α-Glucosidase inhibitory activity of selected Philippine plants. J. Ethnopharmacol., 144:217-219.

Lebovitz, HE. (2001). Effect of the postprandial state on nontraditional risk factors. Am. J. Cardiol., 88:20-25.

Liu, M, Zhang, W, Wei, J, Lin, X. (2011). Synthesis and α-glucosidase inhibitory mechanisms of bis (2, 3-dibromo-4, 5-dihydroxybenzyl) ether, a potential marine bromophenol α-glucosidase inhibitor. Marine Drugs, 9:1554-1565.

Misbah, H, Aziz, AA, Aminudin, N. (2013). Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions. BMC Complement. Altern. Med., 13:118-130.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 26:211-219.

Pimenov, M, Leonov, M. (2004). The Asian Umbelliferae biodiversity database (ASIUM) with particular reference to South-West Asian taxa. Turkish J. Botany, 28:139-145.

Pistia-Brueggeman, G, Hollingsworth, RI. (2003). The use of the o-nitrophenyl group as a protecting/activating group for 2-acetamido-2-deoxyglucose. Carbohyd. Res., 338:455-458.

Rains, JL, Jain, SK. (2011). Oxidative stress, insulin signaling, and diabetes. Free Rad. Biol. Med., 50:567-575.

Semaan, D, Igoli, J, Young, L, Marrero, E, Gray, A, Rowan, E. (2017). In vitro anti-diabetic activity of flavonoids and pheophytins from Allophylus cominia Sw. on PTP1B, DPPIV, alpha-glucosidase and alpha-amylase enzymes. J. Ethnopharmacol., 203:39-46.

Shahidi, F, Naczk, M. (1995). Food phenolics: Food Phenolics: Sources, Chemistry, Effects and Applications. Technomic Publishing Co., Lancaster..

Shim, YJ, Doo, HK, Ahn, SY, Kim, YS, Seong, JK, Park, IS, Min, BH. (2003). Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose. J. ethnopharmacol., 85:283-287.

Singh, P, Jayaramaiah, RH, Agawane, SB, Vannuruswamy, G, Korwar, AM, Anand, A, Dhaygude, VS, Shaikh, ML, Joshi, RS, Boppana, R. (2016). Potential dual role of eugenol in inhibiting advanced glycation end products in diabetes: proteomic and mechanistic insights. Sci. Report., 6:1-13.

Singh, VP, Bali, A, Singh, N, Jaggi, AS, (2014). Advanced glycation end products and diabetic complications. Korean J. Physiol. Pharmacol., 18:1-14.

Son, HU, Lee, SH. (2013). Comparison of α-glucosidase inhibition by Cudrania tricuspidata according to harvesting time, Biomed. Report., 1:624-628.

Staerk, D, Kesting, JR, Sairafianpour, M, Witt, M, Asili, J, Emami, SA, Jaroszewski, JW. (2009). Accelerated dereplication of crude extracts using HPLC–PDA–MS–SPE–NMR: Quinolinone alkaloids of Haplophyllum acutifolium. Phytochemistry, 70:1055-1061.

Yen, GC, Chen, HY. 1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agricult. Food Chem., 43:27-32.

Zarei, MA, Poursharifi, M. (2015). Searching for Alpha-Glucosidase Inhibitory Activity in Hexane Extracts by some Plants from Kurdistan Province. Int. J. Adv. Biol. Biomed. Res., 3:291-296.