Document Type : Original Article
1 Plant Protection Research Department, Ardabil Agricultural and Natural Resources Research and Education Centre, AREEO, Ardabil, Iran
2 Research Assistant Prof. Plant Breeding (Genetics-Biometry), Ardabil Agriculture and Natural Resources Research Centre, Ardabil, Iran
This study was performed in order to determine the distance of isolation for the seed potato production from ware potato in three regions of Ardabil, Namin and Nir. The experiment was carried out in a randomized complete block design (RCBD) with five distances (100, 200, 300, 400 and 500 meters from ware potato) and three replications with Super Elite (SE) class of Agria potato cultivar from ware potato as the source of infection studied by ELISA during 2014 and 2015. According to standard of Seed and Plant Certification and Registration Institute (SPCRI) number of 200 leaves and 200 tubers samples of each distance were collected and their virus in fection was by ELISA assay. Analysis of variance tuber yield showed that between regions, years and distances of isolation were significantly differences. In terms of tuber yield, between distances 500, 400 and 300 meters were not significantly difference and were in one group. With reducing of isolation distance from 500 meters to 100 meters from host of viral diseases vectors (edible potato of Agria cultivar) were decreased tuber yield from 44.328 ton per hectare to 39.828 ton per hectare (about 4.5 ton per hectare, or 10.15 percent). Average infection of Rhizoctonia fungal diseases of tuber in isolation distances and three regions were about 5 percent. In three regions and five isolation distances, tubers were without infection of vascular rot fungus diseases, without infection of bacterial diseases of blackleg and brown rot and without infection of potato moth pest.