Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

Mashayekhan, Shohreh; Jahanshahi, Mahboubeh; Moghadam, Saeed

doi:10.33945/SAMI/IJABBR.2020.3.8

Document Type : Original Article

Authors

¹ Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, 11365-8639, Iran

² 2School of Chemical Engineering, College of Engineering, University of Tehran, Iran

³ 1Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, 11365-8639, Iran

10.33945/SAMI/IJABBR.2020.3.8

Abstract

The role of heart disease in increasing worldwide death and the limited availability of organs for transplantation have encouraged multiple strategies to fabricate functional and implantable constructs. One of these strategies is to develop a biologically similar heart tissue scaffold, in which two types of fiber and hydrogel are commonly used. Toward this goal, taking advantage of both hydrogels properties and fibers features with excellent mechanical properties can be considered as a promising method. The purpose of this study is to develop a fiber/hydrogel composite of gelatin, poly-caprolactone (PCL), cardiac extracellular matrix (ECM), and chitosan. The fibrous scaffolds of PCL and gelatin were characterized by SEM, water drop contact angle test, FTIR, and mechanical tests. The results showed that the average diameter of nanofibers, hydrophilicity and mechanical properties of the fibrous scaffolds increased with increasing the gelatin content in the spinning solution. Furthermore, the results of mechanical tests indicated that by integrating fibers with gelatin to PCL mass ratio of 2 in the hydrogel of chitosan and ECM with a mass ratio equal to 1, we obtained a construct with similar mechanical properties to native heart tissue, which may be proposed as an appropriate scaffold for heart tissue engineering.

Keywords

Main Subjects

Cardiology

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International Journal of Advanced Biological and Biomedical Research

Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

References

References

Volume 8, Issue 3
September and October 2020
Pages 303-314

Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

References

References

Volume 8, Issue 3September and October 2020Pages 303-314

Volume 8, Issue 3
September and October 2020
Pages 303-314