Document Type: Original Article


1 Chemistry Department, Faculty of Science, University of Tripoli, Tripoli, Libya.

2 Chemistry Department, Faculty of Science, University of Tripoli, Tripoli, Libya

3 2Chemistry Department, Faculty of Science, Sebha University, Sebha, Libya


The aim of this study was to evaluate quantitatively the antimicrobial and antioxidant potentials of the leaves extracts of Guava (Psidium guajava Linn.),belonging to the Myrtaceae family. The plant samples were collected from Tajoura eastern part of Tripoli in Libya. Methanol and water were used as solvents in the extraction. The phytochemical screening showed that the ethanolic and aqueous extracts had tannins, flavonoids, alkaloids, saponins, terpenes in varying proportions while alkaloid was the only exclusion in the methanolic extract.
The water, methanol crude extracts as well as flavonoids extract were effective against both Gram- positive and Gram-negative bacteria: Buscillis subtils , Staphylococcus aureus, Escherichia coli and Salmonella typhi.
The minimum inhibitory concentration MIC for flavonoids extract was 2.5 mg/ml .It was also found that both extracts (aqueous and methanolic) of the Guajava leaves showed reasonably high content of polyphenols.
These results suggest that Psidium guajava L. is not only reliable natural source of antimicrobials but also potential sources of phenolic antioxidants and hence could be nominated for further intensive studies


Main Subjects

Aadesariya, MK, Ram, VR, Dave, PN. (2019). Investigation of phytochemicals in methanolic     leaves extracts of Abutilon pannosum and Grewia tenax by Q-TOF LC/MS. Prog. Chem. Biochem. Res., 2:13-19. DOI: 10.33945/SAMI/PCBR.2019.2.1319.

Abdelrahim, SI, Almagboul, AZ, Omer, ME, Elegami, A. (2002). “Antimicrobial activity of Psidium guajava L.,” Fitoterapia, 73:713–715. 

Asemave, K, Anure, T. (2019). The bioactivities of the neem (seeds and leaves) against Callosobruchus maculatus on a Vigna Subterranean L. Prog. Chem. Biochem. Res., 2:92-98. DOI: 10.33945/SAMI/PCBR.2019.3.2.

Dahanukar, SA, Kulkarni, RA, Rege, NN. (2000). Pharmacology of medicinal plants and natural products, Indian J.Pharmacol, 32:S81-S118.

Das, M, Goswami, S. (2019). Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: role of phytochemicals. Int. J. Health Sci. Res., 9(2):39-45.

Daud, A, Gallo, A, Sanchez, A. (2005). Antimicrobial properties of Phrygilanthus acutifolius.  J. Ethnopharmacol., 99:193-197.

Duh, PD, Tu, YY, Yen, GC. (1999). Antioxidant activity of aqueous extract of Hamjyur (Chrsanthemum morifolium Ramat). Lebensmwiss Technol., 32:269-277.

El-Shahaby, O, El-Zayat, M, Rabei, R, Aldesuquy, HS. (2019). Phytochemical constituents, antioxidant activity and antimicrobial potential of Pulicaria incisa (lam.) DC as a folkmedicinal plant. Prog. Chem. Biochem. Res., 2:222-227. DOI:10.33945/SAMI/PCBR.2019.4.7.

Frei, B, Higdon, JV. (2003). Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. J. Nutr., 133(10):755-845.

Fugaban, H. (2016). Comparative study on antifungal property of methanolic and ethanolic extractsof Psidium guajava on Trichophyton mentagrophytes. Schol. Academ. J. Biosci., 4(2):158-160.

Gutiérrez, RM, Mitchell, S, Solis, RV. (2008). Psidium guajava: A review of itstraditional uses, phytochemistry and pharmacology. J. Ethnopharmacol., 117:1–27.

Harborne, JB. (1983). Phytochemical Methods. Chapman and Hall, London, p: 288.

Irith, W, Kai, H, Robert, EW. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc., 3(2):163-175.

Jaiarj, P, Khoohaswan, P, Wongkrajang, Y, Peungvicha, P, Suriyawong, P, Saraya, ML, Ruangsomboon, O.(1999). “Anticough and antimicrobial activities of Psidium guajava Linn. leaf extract,” J. Ethnopharmacol., 67(2):203–212. 

Khan Muluh, E, Odokpe Ugbede, A, Tor-Anyin, TA. (2019). Screening of cassia sieberiana (fabaceae) leaf extract for in-vitro antimicrobial and anti-ulcer activities. Prog. Chem. Biochem. Res., 2:143-149. DOI: 10.33945/SAMI/PCBR.2019.2.7.

Krebs, KG, Heusser, D, Wimmer, H. (1969). In: Stahl, E. (Ed.), Handbook. Springer-Verlag, Berlin, p: 854–905.

Lutterodt, GD. (1992). “Inhibition of Microlax-induced experimental diarrhoea with narcotic-like extracts of Psidium guajava leaf in rats,” J. Ethnopharmacol., 37(2):151–157.

Martha P´erez Guti´errez, R, Mitchell, S, Vargas Solis, R.(2008).Psidium Guajava: A Review of Its Traditional Uses, Phytochemistry and Pharmacology, J. Ethnopharmacol., 117:1-27.

McDevitt, JT, Schneider, DM, Katiyar, SK, Edlind, TD. (1996). In: Program and Abstracts of the 36th Interscience Conference on Antimicrobial Agents and Chemotherapy. American Society for Microbiology, Washington, DC (Abstract 175).

Morais-Braga, MF, Carneiro, JN, Machado, AJ, Dos Santos, AT, Sales, DL, Lim, LF, Figueredo, FG, Coutinho, HD. (2014). Psidium guajava L. from ethnobiology to scientific evaluation: Elucidating bioactivity against pathogenic microorganisms. J. Ethnopharmacol., 194:1140–1152.

Morton, JF. (1987). Fruits of Warm Climates; Creative Resource Systems, Inc.: Winterville, NC, USA.

Naili, MB, Alghazeer, RO, Saleh, NA, AlNajjar, A. (2010). Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea) Arabian J. Chem., 3:79-84.

Ness, AR, Powles, JW. (1997). Fruits and vegetables, and cardiovascular disease: A review. Int. J. Epidemiol., 26:1-13.

Nostro, AM, Germano, A, D’Angelo, NV, Marino, A, Cannatelli, MA. (2000). Extraction Methods and Bioautography for Evaluation of Medicinal Plant Antimicrobial Activity, Lett. Appl. Microbiol., 30:379-384.

Oyaizu, M. (1986). Studies on Product of Browning Reaction Prepared from Glucose Amine. Japan. J. Nutrit., 44:307-315.

Riboli, E, Norat, T. (2003). Epidemiologic evidence of protective effect of fruit and Vegetables on cancer risk. Am. J. Clin. Nutr., 78:5595-5695.

Salazar, DM, Melgarejo, P, Martínez, R, Martínez, J, Hernández, F, Burguera, M. (2006). Phenological stages of the guava tree (Psidium guajava L.). Sci. Hortic., 108:157-161.           

Singleton, VL, Orthofer, R, Lamuela-Raventos, RM. (1999). Analysis of total phenols and oxidization substrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymol., 299:152-177.

Solomon, O, Rabiu Saidu Umar, W, Sanusi Wara, H, Sadiq Yakubu, A, Michael solvent azubuike, M, Asugu Mary, M, Louis, H. (2018). Antiulcerogenic Activity of methanol extract and fractions of Stem Bark of Lannea acida (A. Rich) Against Ethanol-Induced Gastric Mucosal Injury in Albino Rats. Prog. Chem. Biochem. Res., 1:29-39. DOI: 10.33945/SAMI/PCBR.2018.1.2939.

Srinivasan, D, Nathan Suresh, S, Lakshmana, T. (2001). Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J. Ethnopharmacol., 74:217-220.

Stone, B. (1970). The flora of Guam. Micronesica, 6:454–455.

Tsuchiya, H, Sato, M, Miyazaki, T, Fujiwara, S, Tanigaki, S, Ohyama, M. (1996). Comparative study on the antibacterial activity of phytochemical flavanones against Methicillin-resistant Staphylococcus aureus.  J. Ethnopharmacol., 50(1):27-34.

Varley, H. (1976). Determination of alkaline phosphatase. In: Varley, H. (Ed.), Practical ClinicalBiochemistry, fourth ed. p: 453.