Document Type: Original Article

Author

Botany Department, Agricultural and Biological Division, National Research Centre, Dokki, Giza, 33 El Bouhouth St., P.O. 12622, Egypt

10.33945/SAMI/IJABBR.2020.5.2

Abstract

Tomato (Solanum lycopersicum L.) is an important plant rich in many vitamins and antioxidant enzymes. Tomato leaves of two cultivars (‘Peto 86’ and ‘Strain B’) were used as explant sources for callus induction. The antioxidant activity of the calli ethanol (ET) and methanol (ME) extracts were determined. The enzymatic activities were evaluated in callus cultures. Callus induced from leaf explants of tomato cultivars on the Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of cytokinins and auxins such as BAP (6-benzylaminopurine), NAA (1-naphthalene acetic acid), 2,4-D (2,4-dichlorophenoxyacetic acid), IAA (indole-3-acetic acid) and Kin (Kinetin) for rapid induction of callus and biomass growth. The medium (M2) containing 3 mg L-1 BAP with 1 mg L-1 IAA produced the highest percentage of callus induction (PCI) (100%) in two cultivars. The relative fresh weight growth (RFWG) was reported by the fresh callus weighed after four weeks of culture and again weighed after one month of sub-culture. In both cultivars cultured on M2 medium the RFWG was (1.60) in ‘Peto 86’ and (1.47) in ‘Strain B’. The results showed that PCI and RFWG differed with the cultivars tested. The scavenging DPPH free radical activity in callus (ET) extracts exhibited a significant increase in (P < 0.05) than the activity in callus (ME) extract. The peroxidase and polyphenol oxidase activities were found in calli of both tomato cultivars. The enzymatic activities were higher in callus of ‘Peto 86’ cultivar than in callus of ‘Strain B’ cultivar.

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Main Subjects

Abbasi, BH, Khan, MA, Mahmood, T, Ahmad M, Chaudhary, MF, Khan, MA. (2010). Shoot regeneration and free-radical scavenging activity in Silybum marianum L. Plant Cell. Tiss. Organ. Cult., 101:371-376.

Abd El-Hameid, AR, Abo El-kheir, Z, Abdel-Hady, M, Helmy, W. (2018). In vitro and molecular characterization using ISSR markers of Glycyrrhiza glabra L. BioTechnologia, 99(4):409-416.

Abd Elrazig, HE, Musa, M, Elsheikh, SE. (2018). Value chain analysis for tomato production and marketing in Khartoum state, Sudan. Curr. Inves. Agri. Curr. Res., 5(4):715-721. 

Alhasnawi, AN, Che radziah, CMZ, Kadhimi, AA, Isahak, A, Mohamad, A, Yusoff, WMW. (2016). Enhancement of antioxidant enzyme activities in rice callus by ascorbic acid under salinity stress. Biol. Plant., 60 (40):783-787.

Bhatia, P, Ashwath, N, Senaratna, T, Midmore, D. (2004). Tissue culture studies of tomato(Lycopersicon esculentum). Plant Cell Tiss. Organ. Cult., 78:1-21

Boonsiri, K, Saichol, K, Van Doorn, WG. (2007). Seed browning of hot peppers during low temperature storage. Postharvest Biol. Technol., 45:358-365.

Cardoza, V. (2016). Tissue Culture: The manipulation of plant development. In Stewart Jr C.N. (Eds.), Plant Biotechnology and Genetics: Principles, techniques, and applications. New Jersey, NJ:John Wiley & Sons, pp. 113-132. 

Chen, JJ, Yue, RQ, Xu, HX, Chen, XJ. (2006). Study on plant regeneration of wheat mature embryos under endosperm supported culture. Agric. Sci. China, 5:572–578.

Coenen, C, Lomax, TL. (1997). Auxin-cytokinin interactions in higher plants: old problems and new tools. Trends Plant Sci., 2(9):351-356.

Efferth, T. (2018). Biotechnology applications of plant callus cultures. Engineering, 5(1):51-59.

Elimasni, Suryanto D, Rosmayati, Siregar LAM. (2016). In vitro resistant-induction of Tamarillo (Solanum betaceum Cav.) applied by UV-B radiation against anthracnose disease by determination of peroxidase and polyphenol oxidase activity. Inter. J. Sci. Tech. Res., 5(6):26-30.

Ghasemi, S, Koohi, DE, Emmamzadehhashemi, MSB, Khamas, SS, Moazen, M, Hashemi, AK, Amin, G, Golfakhrabadi, F, Yousefi, Z, Yosefbeyk, F. (2018). Investigation of phenolic compounds and antioxidant activity of leaves extracts from seventeen cultivars of Iranian olive(Olea europaea L.). J. Food Sci. Technol., 55(11):4600-4607.

Hanur, VS, Krishnareddy, B. (2016). In-vitro organogenesis in tomato (Solanum lycopersicum) using kinetin. Adv. Plant. Agric. Res., 4(6):397-401.

Jatoi, SK, Sajid, GM, Sappal, H, Baloch, MS, Qureshi, A, Anwar, R. (2001). Differential in vitro response of tomato hybrids against a multitude of hormonal regimes. J. Biol. Sci., 1:1141-1143.

Johnson, M, Wesely, EG, Kavitha, MS, Uma, V. (2011). Antibacterial activity of leaves and inter-nodal callus extracts of Mentha arvensis L. Asia Pac. J. Sci. Technol., 4:196-200.

Kader, A, Sinha, SN, Ghosh, P. (2015). Contribution of environmental factors on in vitro culture of an endangered and endemic mangroves Heritiera fomes Buch.-Ham. and Bruguiera gymnorhiza (L.) Lam. Trop. Plant Res., 2(3):192–203.

Kar, M, Mishra, D. (1975). Catalase, Peroxidase, and Polyphenoloxidase activities    during rice leaf senescence. Plant Physiol., 57:315-319.

Khalid, A, Aftab, F. (2020). Effect of exogenous application of IAA and GA3 on growth, protein content, and antioxidant enzymes of solanum tuberosum L. grown in vitro under salt stress. In vitro Cell. Dev. Biol. Plant, 56:377–389. https://doi.org/10.1007/s11627-019-10047-x.

Kim, HS, Chin, KB. (2017). Evaluation of antioxidative activity of various levels of ethanol extracted tomato powder and application to pork patties. Korean J. Food Sci. Anim Resour., 37(2):242-253. 

Lima, JE, Benedito, VA, Figueira, A, Peres, LEP. (2009). Callus, shoot and hairy root formation in vitro as affected by the sensitivity to auxin and ethylene in tomato mutants. Plant Cell Rep., 28:1169-1177.

Liu, CZ, Murrch, SJ, El-Demerdash, M, Saena, PK. (2004). Artemisia judaica L.: micropropagation and antioxidant activity. J. Biotech., 110:63-71.

Liu, S, Liu, L, Tang, Y, Shuo, X. (2017). Comparative transcriptomic analysis of key genes involved in flavonoid biosynthetic pathway and identification of a flavonol synthase from Artemisia annua L. Plant Biol. J., 19:618-629.

Mazyad, HM, Khalil, EM, Rezk, AA, Abdel-Hakem, MA, Aboul-Ata, AE. (2007). Genetic studies on tomato yellow leaf curl begomovirus (TYLCV) resistance in Egypt: Six pooulation analysis. Int. J. Virol., 3:88-95.  

Moreno, OAV, Vazquez-Duhalt, R, Ochoa, JL. (1989). Peroxidase activity in calluses and cell suspension cultures of radish Raphanus sativus var.Cherry Bell. Plant Cell Tiss. Org. Cult., 18:321-327.

Mostafiz, SB, Waigran, A. (2018). Efficient callus induction and regeneration in selected indica rice. Agronmy, 8:77.

Murashige, T, Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant, 15:473-497.

Oksman-Caldentey, KM, Barz, WH. (Eds.) (2002). Plant Biotechnology and Transgenic Plants. (1 Ed.) CRC Press, pp. 720.

Osman, MG, Elhadi, EA, Khalafalla, MM. (2010). Callus formation and organogenesis of tomato(Lycopersicon esculentum Mill,C.V. Omdurman)induced by thidiazuron. African J. Biotechnol., 9(28):4407-4413.

Pandey, VP, Manika, A, Swati, S, Sameeksha, T, Dwivedi, UN. (2017). A comprehensive review on function and application of plant peroxidases. Biochem. Anal Biochem., 6:308.

Passardi, F, Penel, C, Dunand, C. (2004). Performing the paradoxical: how plant peroxidases modify the cell wall. Trend Plant Sci., 9:534-40.  

Raziuddin, SS, Shah, HJ, Chaudhary, T, Mohammad, AS. (2004). Hormonal effect on callus induction in tomato. Sarhad J. Agri., 20:223-225.

Reda, E, Moghaieb, A, Sneak, H, Fujita, K. (2004). Shoot regeneration GUS transformed tomato (Lycopersicon esculentum) hairy root. Cell Mol. boil., 9:439-449.

Rossignol, M, Santoni, V, Zponanski, S, Wand Vansuyt, G. (1990). Differential sensitivity to auxin at the plasma membrane level. In: NijKamp, HJJ, Van Der Plas, LHW, Van Aartrijk, J, (eds.) Progress in Plant Cellular and Molecular Biology.Kluwer Academic Publisher, Dordrecht, The Netherlands,  pp 498-503.

Rzepka-Plevneš, D, Kulpa, D, Grabiec, M, Kowalczys, K, Kurek, J. (2006). The Effect of Growth Regulators and Culture Conditions on the Callus Induction in Tomato. Acta Sci. Pol. Hortorum Cult., 5(2):23-34.

Shah, SH, Ail, S, Jan, SA, Din, J, Ali, GM. (2015). Callus induction, in vitro shoot regeneration and hairy root formation by the assessment of various plant growth regulators in Tomato(Solanum lycopersicum Mill.). J. Animal Plant Sci., 25(2):528-538.

Sherkar, HD, Chavan, AM. (2014). Studies on callus induction and shoot regeneration in Tomato. Sci. Res. Rep., 4(1):89-93.

Sohail, AJ, Sabir, HS, Shaukat, A, Ghulam, MA. (2015). The effect of plant growth regulators on callus induction and somatic embryogenesis of hybrid tomato. Pak. J. Bot., 47(5):1671-1677.

Swamy, MK, Mohanty, SK, Anuradha, M. (2014). The effect of plant growth regulators and natural supplements on in vitro propagation Pogostemon cablin Benth. J. Crop Sci. Biotech., 17(2):71-78.

Tagi, A, Kumar, PP, Lakshmanan, P. (2002). In Vitro Plant Breeding, Food Products Press, New York, pp 167.

Tanveer, H, Ali, S, Khan, Z. (2012). Appraisal of secondary metabolites in in vitro cultures of Citrullu scolocynthis (L.). Schard Sci. Int. (Lahore), 24(1):75-80.

Ullah, AA, Tungmunnithum,D, Garros, L, Drouet, S, Hano, C, Abbasi, BH. (2019). Effect of ultraviolet-c radiation and melatonin stress on biosynthesis of antioxidant and antidiabetic metabolites produced in in vitro callus culture of Lepidium sativum L. Int. J. Mol. Sci., 20:1787.

Umesh, TG. (2014) In vitro callus induction and antioxidant potential of Decalepis hamiltonii (Wight and Arn). Int. J. Pharm. Sci., 6:452-456.

Wang, RA, Zhang HB, Tanksley SD. (1994). Map based cloning in crop plants tomato as a model system, Genetic and Physical mapping of jointless. Mol. Gen. Genet., 242:681-688.  

Yousaf, R, Khan, MA, Ullah, N, Khan, I, Hayat, O, Shehzad, MA, Khan, I, Taj, F, Ud Din, N, Khan, A, Naeem, I, Ali, H. (2019). Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia. Artif. Cell. Nanomed. Biotechn., 47(1):1122-1131.