Evaluation of Flavonol Synthase (FLS) Gene Expression in Cressa cretica L. Using Plant Growth Regulators

Naderi, Davoud; Solouki, Mahmood; Fakheri, Baratali

doi:10.22034/ijabbr.2021.44771

Document Type : Original Article

Authors

¹ Agriculture Biotechnology Research Institute, University of Zabol, Zabol, Iran

² Department of Biotechnology and Plant Breeding, Faculty of Agriculture, University of Zabol, Zabol, Iran, P.O. Box 9861335856,

https://doi.org/10.22034/ijabbr.2021.44771

Abstract

Background: C. cretica is a medicinal and ornamental halophytic plant which used for alterative, anthelmintic, tonic and aphrodisiac purposes. The purpose of this study was to detect the quercetin content in C. cretica and to evaluate the effects of exogenous application of abscisic acid and cytokinin hormones on FLS gene expression.
Methods: Abscisic acid and cytokinin (BAP) was sprayed in two-stages (8 days intervals) with two different concentration of 300 and 500 ppm. Quercetin content was confirmed by HPLC analysis in aerial parts of plant.
Results: The findings revealed that gene expression of FLS increased in the second stage of abscisic acid treatment. Observation showed significant differences in gene expression between treated and control samples, at level of 1%. In the first phase, there were no significant differences between the abscisic acid treated and control samples. In addition, the cytokinin treatment (500 ppm) resulted to the expression of the FLS gene at 5% level, which implied that cytokinin affected the FLS gene expression.
Conclusions: In this research, it was proved that the dihydroquercetin was present in C. cretica which changed to quercetin through the flavonol synthase enzyme.

Graphical Abstract

Keywords

References

1. Chopra RN, Nayer SL, Chopra IC. (2006). Glossary of India Medicinal Plants. National institute of icience communication and information resource, (CSIR). New Delhi. 80 Pages

2. Zia S, Khan MA. (2004). Effect of light, salinity, and temperature on seed germination of Limonium stocksii. Can. J. Bot., 82, 151-157, https://doi.org/10.1139/b03-118.

3. Forkmann G. (1991). Flavonoids as flower pigments: the formation of the natural spectrum and its extension by genetic engineering. Wiley online library, Plant Breeding Book. 106 (1): 1–26. https://doi.org/10.1111/j.1439-0523.1991.tb00474.x

4. Sakanashi Y. (2008). Possible use of quercetin, an antioxidant, for protection of cells suffering from overload of intracellular Ca2+: a model experiment. J. Life Sci., 83, 164-169.

5. Ban T, Ishimaru M, Kobayashi S, Shiozaki S, Goto-Yamamoto N, Horiuchi S. (2003). Abscisic acid and 2,4-dichlorophenoxyacetic acid affect the expressionof anthocyanin biosynthetic pathway genes in ‘Kyoho’ grape berries. J. Hortic. Sci. Biotech., 78, 586-589.

6. Jeong ST, Goto-Yamamoto N, Kobayashi S, Esaka M. (2004). Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins. Plant Sci. J., 167, 247–252.

7. Leung J, Giraudat J. (1998). Abscisic acid signal transduction. J. Annu. Rev. Plant Physiol., 49, 199–222. https://doi.org/10.1146/annurev.arplant.49.1.199.

8. Deytieux-Belleau C, Gagne SL, Hyvernay A, Doneche B, Geny L. (2007). Possible roles of both abscisic acid and indoleacetic acid in controlling grapeberry ripening process. J. Int. Sci. Vigne. Vin., 41, 141–148. DOI: 10.20870/oeno-one.2007.41.3.844.

9. Li X, Kim Y B, Kim Y, Zhao S, Kim H H, Chung E, Lee J-H, Park S U. (2013). Differential stress-response expression of two flavonol synthase genes and accumulation of flavonols in tartary buckwheat. J. Plant Physiol., 170, 1630-1636.

10. Toh HC, Wang SY, Chang ST, Chu FH. (2013). Molecular cloning and characterization of flavonol synthase in Acacia confuse. Tree Genet. Genomes J., 9, 85-92.

11. Fang F, Tang K, Huang WD. (2013). Changes of flavonol synthase and flavonol contents during grape berry development. Eur. Food Res. Technol. J., 4, 529-540.

12. Amandeep KS, Dennis J, andJiang Lu. (2011). Effects of exogenous abscisic acid on antioxidant capacities, anthocyanins, and flavonol contents of muscadine grape (Vitisrotundifolia) skins. Food Chem. J. 126, 982-988.

13. Shahat AA, Abdel Azim NS. (2005). Flavonoids from Cressa cretica. Pharm. Biol. J. 42, 349-352.

14. Liu HP, Shi XF, Zhang YC, Li ZX, Zhang L, Wang ZY. (2011). Quantitative Analysis of Quercetin in Euphorbia helioscopia L by RP-HPLC. Cell. Biochem. Biophys., 61, 59–64.

15. Dueñas M, Mingo-Chornet H, Pérez-Alonso J J, Di Paola-Naranjo R, González-Paramás A M, Santos-Buelga C. (2008). Preparation of quercetinglucuronides and characterization by HPLC–DAD–ESI/MS. Eur. Food Res. Technol. J., 227, 1069–1076.

16. Chomczynski P, Sacchi N. (1987). Single-step method of RNA isolation by acid guanidine ium thiocyanate-phenol chloroform extraction. J. Anal. Biochem., 162, 156–159.

17. Kalinowska E, Chodorska M, Paduch-Cichal E, Mroczkowska K. (2012). An improved method for RNA isolation from plants using commercial extraction kits. Acta Biochim. Polon. J., 59, 391-393.

18. Loomis MD. (1974). Overcoming problems of phenolics and quinines in the isolation of plant enzymes and organelles. Methods Enzymol., 31, 528–544.

19. Ding LW, Sun QY, Wang ZY, Sun YB, Xu ZF. (2008). Using silica particles to isolate total RNA from plant tissues recalcitrant to extractionin guanidine thiocyanate. Anal. Biochem. J., 374, 426–428.

20. Tong Z, Qu S, Zhang J, Wang F, Tao J, Gao Z, Zhang Z. (2012). A Modified Protocol for RNA extraction from different peach tissues suitable for gene isolation and Real-Time PCR analysis. Mol Biotechnol. J., 50, 229–236.

21. Tan SC, Yiap BC. (2009). DNA, RNA, and protein extraction: the past and the present. J. Biomed. Biotechnol., 2009, 1-10.

22. Bustin SA, Benes B, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. (2009). The MIQE guideline: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. J., 55, 611–622.https://doi.org/10.1373/clinchem.2008.112797.

23. Li X, Kim YB, Kim Y, Zhao S, Kim HH, Chung E, Lee JH, Park SU. (2013). Differential stress-response expression of two flavonol synthase genes and accumulation of flavonols in tartary buckwheat. J. Plant. Physiol., 170, 1830-1836.

24. Liu W, Saint DA. (2002). Validation of a quantitative method for real time PCR kinetics. Biochem. Biophys. Res. Commun., 294, 347–353.

25. Peirson S N, Butler J N, Foster R G. (2003). Experimental validation of novel and conventional approaches to quantitative real‐time PCR data analysis. Nucleic. Acids Res. J., 31, e73.

International Journal of Advanced Biological and Biomedical Research

Evaluation of Flavonol Synthase (FLS) Gene Expression in Cressa cretica L. Using Plant Growth Regulators

References

References

Volume 9, Issue 1 - Serial Number 1
January 2021
Pages 64-76

Evaluation of Flavonol Synthase (FLS) Gene Expression in Cressa cretica L. Using Plant Growth Regulators

References

References

Volume 9, Issue 1 - Serial Number 1January 2021Pages 64-76

Volume 9, Issue 1 - Serial Number 1
January 2021
Pages 64-76