Document Type: Original Article


1 Post graduate student of the Faculty of Veterinary Medicine, University of Tabriz-Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz-Iran


Postpartum anestrous is one of the most important problems in the dairy industry in the world and especially in Iran. The objective of present study was to compare the efficacy of two hormonal programs for recycling of postpartum anestrous cows. For this reason, 230 Holstein cows from 4 similar dairy herds (with totally 2560 cow and unique management and feeding system) located on the suburb of Tabriz (North-west of Iran) were examined during April 2012 to August2013. These cows showed no visible oestrus signs until the minimum 60 days postpartum. Clinical examinations were performed twice, with a 10 days interval, and blood samples for progesterone analysis were collected simultaneously with every rectal palpation. Based on progesterone values and clinical examinations only cows with lower progesterone levels (P4<2ngr/ml) at both sampling and nonfunctional ovaries (at two consecutive examinations) were randomly allocated into three groups: A, B (n=100) and C (n=30). Cows in group A were administered a combination of Gonadotropin releasing hormone (GnRH) on day 1 and prostaglandin F2alpha on day 8; cows in group B were administered 125 mg of progesterone for 8 days, whereas cows in the control group C were given placebo. Subsequently all cows were examined for signs of estrus and performed artificial insemination on two consecutive estrus following treatment. In groups A & B  the rates of observed oestrus, first service conception rates and second service conception rates were 5%  vs. 68%, 60%  vs. 25% and100% vs. 64.7% respectively. In the group of C, cows were not observed in estrous.  Significant differences were observed between groups in all fields (p≤0.01). Thus based on the current study it can be concluded that for recycling postpartum anoestrus cows, progesterone therapy is the treatment of choice.


Bo G.A. and Baruselli P.S. (2002). Programas de inseminación artificial a tiempo fijo en el ganado bovino en regiones subtropicales y tropicales in: C. González-Stagnaro, E. Soto-Belloso, L. Ramírez-Iglesia (Eds.), Avances en la Ganadería de Doble Propósito, Fundación GIRARZ, Ediciones Astro Data S.A., Maracibo, Venezuela, pp. 497–514.

Butler WR. (2000).Nutritional interactions with reproductive performance in dairy cattle. Anim. Reprod. Sci. 60-61:449-457.

Chupin D., BALL J. and Thimonier J. (1975). The control of reproduction in the nursing cow with a short-term progestagen Treatment, Annales de Biologie annimal. Biochimie, Biophysique, 15; 263-274.

De Ondiz A., Perea F., Cruz R., Portillo G. and Soto E. (2002). Evaluación ultrasonográfica del crecimiento del folículo ovulatorio en vacas anéstricas mestizas cebú post-tratamiento con norgestomet y eCG. Arch. Latinoam. Prod. Anim. 10 (1); 20–23

DORAY J.M., SCHIERSMANN G.C.S. and Torquati O. (1991). Effects of progesterone-estradiol Treatment on postpartum reproductive performance on primiparous and multiparous beef cows. In: Proceedings XXIV World Veterinary Congress. S~ao Paulo, Brazil, Abstr. 209.

Gröhn YT., Hertl JA. and Harman JL. (1994).Effect of early lactation milk yield on reproductive disorders in dairy cows. A. J. Vet. Res. 55;1521-1528.

Macmillan KL., Laen IJ. and Westwood CT. (1996).The effects of lactation on the fertility of dairy cows. Aust. Vet. J. 73; 141-147.

Odde, K.G. (1990). A review of synchronization of estrus in postpartum cattle, J. Anim. Sci. 68; 817-830.

Palomares R., Perea F., Hernández H., Sandoval J., De Ondiz A., Goicochea J., Gonzalez R. and Soto E. (2004). Prevention of anestrus using an intravaginal progestagen device combined with 17β-Estradiol, GnRH and PGF at 60 days postpartum in dual purpose crossbreed zebu cows. Reprod. Fertil. Dev. 16, p. 131 (Abstract).

Pösö J. and Mäntysaari EA. (1996).Genetic relationships between reproductive disorders, operational days open and milk yield. Livest. Prod. Sci. 46; 41-48.

Rhoads ML., Rhoads RP., Gilbert RO., Toole R. and Butler WR. (2006). Detrimental effects of high plasma urea nitrogen levels on viability of embryos from lactating dairy cows. Animal. Reprod. Sci. 91;1-10.

Soto E., González R., Portillo G. and Ramírez L. (1998). Uso de los dispositivos intravaginales CIDR para el tratamiento del anestro en vacas mestizas doble propósito .Revista Científica FCV-LUZ, 8; 84–86.

Soto E., Portillo G., De Ondiz A., Rojas N., Soto G., Ramírez L., Aranguren J. and Perea F. (2000). Evaluación del comportamiento reproductivo mediante el uso de la progesterona por radioinmunoanálisis en vacas mestizas cebú bajo programas de inseminación artificial en Venezuela. Revista Científica FCV-LUZ, 10 (5); 391–398.

Yavas Y. and Walton J.S. (2000). Postpartum cyclicity in suckled beef cows: A review. Theriogenology, 54, pp. 25–55.