Document Type: Original Article

Authors

1 M.sc. Student of Animal Nutrition, Department of Animal Science, University of Yasouj, Iran

2 Department of Animal Science, Faculty of Agriculture, University of Yasouj, Iran

Abstract

Oak trees cover a wide range of forests in Iran and most of them are located in Kohgiloye and Boyer-Ahmad province (K.B). The high tannin is one of the anti-nutritional characteristics of acorn for domestic animal. Wide range of gut microorganisms could tolerate tannin and reduce it by producing tannase enzyme. This study was carried out to isolate and identify the tannin resistance bacteria (tannase enzyme producing bacteria) from feces samples of domestic sheep and goat. For this purpose, twenty six feces samples of native sheep and goats were collected through the province. Firstly, samples were diluted and then 0.1 ml of each suspension (dilutions) was cultivated on the tannin-treated brain heart infusion agar media containing tannin and then was incubated at 37 C for 72 hours under anaerobic conditions. The colonies were isolated and identified by biochemical experiments and API (20 STREP) test. The results showed that five strains of Streptococcus pneumonia and 2 strains of Streptococcus bovis having tannin hydrolysis activity.  

Keywords

Aguilar, C. N., Rodriguez, R., Gutierrez-Sanchez, G., Augur, C., Favela-Torres, E., Prado-Barragan, L.A., Ramirez-coronel, A and Contreras-Esquivel J.C (2007). Microbial tannanes: advances and perspective. Applied Microbiol Biotechnology. 76: 47-59. 

Allison, M.J., Hammond, A.C and Jones R.J (1990). Detection of ruminal bacteria that degrade toxic dihydroxypyridine compounds produced from mimosine. Applied and Environmental Microbiology. 56:590–594.

Bojar Pour, M., Bahmaninia, E., Favazi, J and Ebrahimi R (2010). Evaluate effects of different inclusion of oak kernel with determent food potential oak kernel substitute with corn seed on broiler chickens ration. Research Journal of Biological Science. 5: 17-19.

Brooker, J., DO’Donovan, L.A., Skene, I., Clarke, K., Blackall, L and Muslera, P (1994). Streptococcus caprinus sp. nov., a tannin-resistant ruminal bacterium from feral goats. Letters in Applied Microbiology. 18:313–318.

Eden, E., Odenyo, A and Ashenafi, M (2005). Isolation and characterization of tannin-degrading bacteria from faecal samples of some wild ruminants in Ethiopia. Animal Feed Science and Technology. 118 : 243–253.

Field, J.A.  and Lettinga, G (1987). The methanogenic toxicity and anaerobicdegradability of a hydrolyzable tannin. Water Res. 21:367–374.

Huang, L., Forsberg, C.W., Lam, J.S and Cheng, K.J (1990). Antigenic nature of the chloride-stimulated cellobiosidase and other cellulases of Fibrobacter succinogenes subsp. succinogenes S85 and related fresh isolates. Applied and Environmental Microbiology. 56:1229–1234.

Makkar, H.P.S (2003). Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research. 49: 241-256.

Maldonado, R.A.P and Norton, B.W (1996). The effects of condensed tannins form desmodium intortum and calliandraeal othyrsus on protein and carbohydrate digestion in sheep and goats. British Journal of Nutrition. 76: 515-533

Nelson, K.E., Pell, A.N., Schofield, P and Zinder, S (1995). Isolation and characterization of anaerobic ruminal bacterium capable of degrading tannins. Applied and Environmental Microbiology. 61, 3293–3298.

Nemoto, K., Osawa. R., Hirota, K., Ono, T and Miyake, Y (1995). An investigation of gram-negative tannin-protein complex degrading bacteria in fecal flora of various mammals. Journal of Veterinary Medical Science. 57:921–926.

Nitiema, L.W., Dianou, D., Simpore, J., Karou, S.D., Savadogo P.W and Traore, A.S (2010).  Isolation of a Tannic Acid-Degrading Streptococcus sp. From an Anaerobic Shea Cake DigesterPakistan Journal of Biological Sciences. 13(1): 46-50.

Osawa, R (1990). Formation of a Clear Zone on Tannin-Treated Brain Heart Infusion Agar by a Streptococcus sp. Isolated from Feces of Koalas." Applied and Environmental Microbiology. 56: 829- 831.

Osawa, R (1992). Tannin-protein complex-degrading enterobacteria isolated from the alimentary tracts of koalas and a selective medium for their enumeration. Applied and Environmental Microbiology. 58:1754–1759.

Osawa, R and Sly, L.I. (1992). Occurrence of tannin protein complex degrading Streptococcus sp. in various animals, Systematic and Applied Microbiology. 15 :144–147.

Skene, I.K and Brooker J.D (1995). Characterization of tannin acylhydrolase activity in the ruminal bacterium Selenomonas ruminantium. Anaerobe 1, 321–32.