Document Type: Original Article

Authors

1 Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan

2 School of Pharmacy, The University of Faisalabad, Faisalabad, Pakistan

3 University Of Agriculture, Faisalabad (Sub campus Dera Ghazi Khan), Pakistan

Abstract

The methanolic leaf extract of Thuja occidentalis was evaluated for possible sedative activities in mice. Sedative activity was evaluated by using hole cross, open field, thiopental sodium-induced sleeping time and elevated-plus maze (EPM) tests at 200mg/kg and 400 mg/kg.  The extract decreased the locomotor activity of mice in hole cross, open field and EPM test and showed the remarkable results as compared to the standard at both mentioned doses. Moreover, the extractsignificantly minimized onset of sleep and maximized the duration of sleeping time when administered with thiopental sodium and statistically it was significant (p < 0.05).

Keywords

Amos, S., Kolawole, E., Akah, P., Wambebe, C. andGamaniel, K. (2001). Behavioral effects of the aqueous extract of Guiera senegalensis in mice and rats. Phytomedicine, Vol. 8(5): 356-361.

Aziz. A, Khan. I.A. (2013). “ Pharmacological evaluation of Sedative and Hypnotic activities of methanolic extract of Lycopus europaeus in mice”.  The Journal of Phytopharmacology . 2(4): 8-12.

Aziz A., Khan I. A., Munawar S. H., Sadr-ul-Shaheed. (2013). “Antipyretic study of methanolic bark extract of plumeria rubra, linn. In various pyrexia induced models”, Int. J. Res. Dev. Pharm. L. Sci. 2(6), pp. 680-685.

Belal N, Bodinet C, Tegtmeier M & Lindequist U. (2005). Thuja occidentalis(Arbor vitae): A Review of its Pharmaceutical,Pharmacological and Clinical Properties. Advance AccessPublication. 69-78.

Chang LC, Song LL, Park EJ, Luyengi L, Lee K J& Norman R. (2000). Bioactive Constituents of Thuja occidentalis. Journal of Natural Product.  63:1235.

Nam SH & Kang MY. (2005).  Antioxidant activity of Medicinal Plants. Pharmaceutical Biotechnology 42: 409.

 Deb L, Dubey SK, Jain AK, Jain A, Pandian GS & Rout SP.( 2007). Antidiarrhoeal activity of Thuja occidentalis Linn. Ethanol extract on experimental animal. Indian Drugs. 44:319.

Johnston, G.A.R. (2005). GABAA Receptor Channel Pharmacology. Current Pharmaceutical Design, 11:1867-1885.

Gottesmann, C. (2002). GABA mechanisms and sleep. Neuroscience 111, 231–239.

Lister RG. (1987). The use of a plus-maze to measure anxiety in the mouse.Psychopharmacology(Berl) 92, 180-185.

Tobler, I. Kopp, C., Deboer, T. and Rudolph, U. (2001). Diazepam-induced changes in sleep, Role of α1 GABAA receptor subtypes. PNAS., 98(11): 6464-6469.

WHO/EDM/TRM .(2000).1 General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine pp.1-20.

Wheatley, D. (2005). Medicinal plants for insomnia: a review of their pharmacology, efficacy and tolerability. Journal of Psychopharmacology, 19 (4): 414-421.