Document Type: Original Article


1 Young Researchers Club, Islamic Azad University, Science and Research Branch, Ardabil, Iran

2 Young researchers club, Boroujed branch, Islamic Azad University, Boroujerd, Iran


A study was performed to study the effect of drought stress on protein changes of five wheat genotypes. Wheat genotypes were planted in early November, growing season of 2008-2009. The experiment was carried out in a factotial design with three replications. Two drought stress levels (irrigated and Non irrigated) and five genotype of wheat (F103-L-1-12//PONY/OPATA, F1.158/FDL//BLO/3/SH14414/CROW/4/C ICWH99381-0AP-0AP-OMAR-6MAR, PYN/BAU//VORONA/HD2402, KATILA-13, and SARDARI- HD35/5/DMN//SUT/AG(ES86-7)/3/ICWH99-0552-0AP-0AP-OMAR-3MAR) factorialed in plots as random. Proteins profiling of samples was performed using SDS- polyacrylamide gels. The results showed that. In all genotypes the effects of drought stress on seed storage proteins were significant. Also results shows that, in drought stress treatment the seed storage proteins was more than irrigated treatment. No effects drought stress treatments on protein banding patterns. Also, results indicated that not obvious any new band and not deleted any bands. Therefore we concluded that seed protein bands are very stable in front of environment changes.


Ashraf. M.Y. I (1998b). Photosynthetic efficiency of wheat under water stress conditions. Pak. J. Scientific and Industrial Res. 41: 15 1-163.

Beyranvand, H., Farnia, A., Nakhjavan, SH. and Shaban, M. 2013. Response of yield and yield components of maize (Zea mayz L.) to different bio fertilizers. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 9: 1068-1077.

Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities ofprotein utilizing the principle of protein-dye binding. Ann. Biochem., 72: 248-254.

Cattivelli, L., F. Rizza, F.W. Badeck, E. Mazzucotelli, A.M. Mastrangelo, E. Francia, C. Mare., A. Tondelli and A.M. Stanca, (2008). Drought tolerance improvement in crop plants: An integrated view from breeding to genomics. Field Crop Res., 105: 1-4.

Iqbal, S.H., A. Ghafoor and N. Ayub. (2005). Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pak. J. Bot., 37: 87-96.

Javid, A., A. Ghafoor and R. Anwar. (2004). Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pak. J. Bot., 36: 87-96.

Karim, M.F. and Q.A. Fattah, (2006). Changes inyield of chickpea biocomponents of chickpea (Cicer arietinum L.)sprayed with potassium napthenate and napthenicacetic acid. Bangladesh J. Bot., 35(1): 39-43.

Kiani, M, Farnia, A.,and Shaban, M. (2013). Changes of seed yield, seed protein and seed oil in rapeseed (Brassica napus L.) under application of different bio fertilizers. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 10: 1170-1178.

Kim CS, Kamiya S, Sato T, Utsumi S, Kito M (1990). Improvement of nutritional value and functional properties of soybean glycinin by protein engineering. Protein Eng. 3: 725-731.

Laemmli, U.K. (1970). Cleavage of structure proteins assembly of the head of bacteriophage T4.Nature, 22: 680-685.

Luo C, Branlard G, Griffin WB, McNeil DL (2000) The effect of nitrogen and sulphur fertilization and their interaction with genotype on wheat glutenins and quality parameters. J Cereal Sci 31: 185–194.

Ottman MJ, Doerge TA, Martin E (2000) Durum grain quality as affected by nitrogen fertilization near anthesis and irrigation during grain fill. Agron J 92: 1035–1041.

Prihar SS, Sandhu BS (1968) Irrigation of field crops, Indian Council of Agric. Res New Delhi 142.

Rharrabti Y, Villegas D, Garcia Del Moral LF, Aparicio N, Elhani S, Royo C (2001) Environmental and genetic determination of protein content and grain yield in durum wheat under Mediterranean conditions. Plant Breed 120:381–388.

Shaban, M. (2013a). Application of seed equilibrium moisture curves in agro physics. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 9: 885-898.

Shaban, M. (2013b). Biochemical aspects of protein changes in seed physiology and germination. International journal of Advanced Biological and Biomedical Research. Volume 1, Issue 8: 885-898.

Singh KB, Malhotra RS, Halila MH, Knights EJ, Verma MM (1994) Current Status and Future Strategy in Breeding Chickpea for Resistance to Biotic and Abiotic Stresses, in Expending the Production and Use of Cool Season Food Legumes, Eds. F.J. Muehlbauer and W.J. Kaiser, Klwer Academic Pub., printed the Netherlands, p:572-591

Singh, A.K., R.K. Chaudhry and R.P.R. Shaema, (1993). Effect of inoculation and fertilizer level on yield, nutrients uptake and economics of summer pulses. Ind. J. Potassium Res., 9: 176-178.

Suoyi Han, Rui Fan, Tuanjie Zhao, Jingjing Yu and Deyue Yu.( 2009). Seed storage protein components are associated with curled cotyledon phenotype in soybean. African Journal of Biotechnology Vol. 8 (22), pp. 6063-6067.

Tanksley S.D., Jones R.A. (1981) Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort. Sci., 16: 179-181.