Document Type: Original Article


1 Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Center of comparative&experimental medicine, Basic sciences in infection diseases research center, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran

3 School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran


Pathogens are an important and significant hazard for human and animal health. In recent years, antibiotics are used to treat different types of infection caused by bacterial agents. In veterinary medicine, antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease, but the adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of florfenicol drug in embryonated eggs. The objective of this study was to investigate using of various dosages of florfenicol solution for in ovo administration in chicken embryo. Fertile chicken eggs were divided into four equal treatment groups as follows: group 1: no injected group. Group 2: phosphate buffered saline-injected group; whose individuals were injected with phosphate buffered saline. Groups 3 and 4 whose individuals were injected with florfenicol injectable solution at a dosage of 20 and 30 mg per Kg egg-weight, respectively. Embryos were re-incubated post-treatment and allowed to develop until day 18 after which; they were examined for macroscopic and microscopic lesions. Results showed that embryos were normal in all treatment groups. Microscopically, no lesions were also diagnosed in tissues. Based on macroscopic and microscopic findings, it is concluded that florfenicol at above-mentioned concentration is not toxic for the chicken embryo. So, florfenicol egg-injection can be used toeliminate pathogens and prevention of egg transmission of the diseasewithout any adverse effect.


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