Document Type: Original Article

Authors

1 Graduate MSc of Agricultural Biotechnology, Razi University, Kermanshah, Iran

2 Assistant Professor, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

3 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Abstract

Drought stress in plants, the change (increase or decrease) in the production of plant proteins. Proteomics in recent years one of the most powerful tools that help us to study the changes in protein In order to investigate the proteome of wheat leaves in response to terminal drought, two genotypes susceptible and resistant wheat genotypes were evaluated under irrigated (non-stress) and rain-fed (stress) conditions. After applying stress and extraction of leaf proteins, two-dimensional gels were prepared and scanned. Analysis of gel images was performed using Same Spot Progenesis. About 657 protein spots were identified by the software. After alignment of the spots and their correspondence, 148 spot were identified visually and by using the software and statistical analysis was carried out. Five spots with Fold ≥ 1/5 at P ≤ 0/05 were identified, of which 4 spots were significant at P ≤ 0/05 and 1 spot was significant at P ≤ 0/01.

Keywords

Main Subjects

Bradford, M. M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein dye binding.Anal Biochem. 72: 248-254.

Chaves, M.M., M.M. Oliveira (2004) Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture, J. Exp. Bot., 55, N 407, 2365-2384.

Damerval C., D. deVienne, M. Zivy and H. Thiellement (1986) Technical improvements in two- dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis. 7:52-54.

Demirevska, K., L. Simova-Stoilova, V. Vassileva,  I. Vaseva, Grigorova  and  B. Feller (2008) Drought- induce leaf protein alterations in sensitive and tolerant wheat varieties. J. plant physiology. 34:79-102.

Donnelly. B., R. Madden, P. Ayoubi, D. Porter and J. Dillwith (2005) The wheat

(Triticum aestivum L.) leaf proteome. J. Proteomics. 5: 1624–1633.

Farshadfar, E., H. Ghasempour and H. Vaezi (2008) Molecular aspects of drought tolerance in bread wheat (T. aestivum). Pak. J. Biol. Sci. 11(1): 118-112.

Feller, U., I. Anders and T. Mae  (2008) Rubiscolytics: fate of Rubisco after its enzymatic function in a cell is terminated. J. Exp. Bot., 59: 1615-1624.

Gorg, A., W. Weiss and M.J. Dunn (2004) Current two-dimensional electrophoresis technology for proteomics. Proteomics, 4: 3665–3685.

Haj Heydari M, E`yvazi A, Hosseini Salekdeh Gh  (2005) Proteome analysis of wheat seeds under drought stress. National

Biotechnology Congress of Iran (In Farsi).

Heikkila, J. J., J. E. T. Papp, J. A. Schultz and J. D. Bewley (1984) Introduction of heat shock protein messenger RNA in maize mesocotyls by water stress, abscisic acid and wounding. Plant Physiology, 76:270- 274.

Isaacson, T., C.M.B. Damasceno, R.S. Saravanan, Y. He, C. Catala, M. Saladie and J.K.C. Rose (2006) Sample extraction techniques for enhanced proteomic analysis of plant tissues. Nat. Protocols, 1: 769–774.

Jangpromma.N., S. Kitthaisong, S. Daduang, P. Jaisil and S.Thammasirirak (2007) 18kDa protein accumulation in sugarcane leaves under drought stress conditions. KMITL Sci. Tech. J.Voi. 7No. S1 Nov. pp. 44-54.

Li. Q., J. Huang, Sh. Liu, J. Li1, X. Yang, Y. Liu and Zh. Liu (2011) Proteomic analysis of  young leaves at three developmental stages in an albino tea cultivar. Proteome Science, 9-44.

Li. X. J.,M. F. Yang, Y. Zhu, Y. Liang  and Sh. H. Shen (2011) Proteomic analysis of salt stress responses in rice shoot. J. Plant Biol.

Sivamani, E., A. Bahieldin, J. M. Wraith, T. Al- Niemi, W. E. Dyer, T. H. D. Ho and R. Qu (2000) Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene.

Sujin, Ray wu (2004) Stress-inducible synthesis of proline in transgenic rice confers growth under stress conditions than that with constitutive synthesis. Plant Sci., 166(4): 941-948.

Suoyi, H.,  F. Rui, Z. Tuanjie, Y. Jingjing and Y. Deyue (2009) Seed storage protein components are associated with curled cotyledon phenotype in soybean. African J. of Biotechnology Vol. 8 (22), pp. 6063-6067.

Zimmerman, M. H (1998) Hydraulic architecture of some diffuse propous trees. Can. J. Bor. 56: 2286-2295.