Document Type: Original Article

Authors

Department Plant Pathology, Faculty of Agricultural Science and Engineering, University of Tehran, College of Agriculture and Natural Resources, Karaj, Iran

Abstract

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran province.
The polyclonal antibodies were used to the samples by DAS-ELISA . Some of the tested samples, showed positive reaction against cucumber mosaic virus (CMV) specific antibody. Some of the positive samples which are mechanically inoculated on test plants.Some leaf samples from mint plants with symptoms of yellows and mosaic were showed positived reaction against cucumber mosaic virus specific antibody.To confirm the identify of the virus detected using a specific primer pair of CMV which amplify part of coat protein of CMV . A fragment about 700bp was amplified in Immunocapture reverse transcriptase polymerase chain reaction.(IC-RT-PCR).

Keywords

Bennet, C. W. 1944. Studies of dodder transmission of plant viruses. Phytopathology, 34:905-932.

Clarck, M. F. and Adams, A. N. 1977. Characteristic of microplate method of enzyme – linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol, 34:475-483.

DeAngelis, J. D., Sether, D. M., and Rossignol, P. A. 1993. Survival, development, and reproduction in western flower thrips (Thysonoptera,Thripidae) exposed to impatiens necrotic spot virus. Environ. Entomol. 22:1308-1312.

Fletcher, J. D. 2001. New hosts of Alfala mosaic virus, Cucumber mosaic virus, Potato virus Y, Soybean dwarf virus, and Tomato spotted wilt virus in New Zealand. N. Z. J. Crop Hortic. Sci. 29:213-217.

Fletcher, J. D. 2001. New hosts of Alfalfa mosaic virus, Cucumber mosaic virus ,Potato virus Y,Soybean dwarf virus,Tomato spotted wilt virus in New Zealand. N. Z. J. Crop Hortic. Sci. 29:213-217.

Francki, R. I. B.,Fequet, C., Knudson, D. L., and Brown, L. 1991. Classification and nomenclature of viruses fifth report of the international committee for taxonomy of viruses, Arch. Virol. Supplementum2.Springer-Verlag,Wien.450pp.

Francki, R. I. B. 1968. Inactivation of Cucumber mosaic virus (Q Strain)nucleoprotein by pancreatic ribonuclease. Virology,34:694-700.

Francki, R. I. B.,Fequet, C., Knudson, D. L., and Brown, L. 1991. Classification and nomenclature of viruses fifth report of the international committee for taxonomy of viruses, Arch. Virol. Supplementum2.Springer-Verlag,Wien.450pp.

Francki, R. I. B., Mossop, D. W., and Hatta, T. 1979. Cucumber mosaic virus. Description of plant viruses. No.213.

Han-Xin, L., Rubi, L.,Smyth, B., and Bryce, W. F. 2004. Molecular population Genetics of Cucumber mosaic virus in California Evidence for Founder Effects and Ressortment. Journal of Virology, 78:12.

Plukaitis, P., Roossink, M. J.,Dietzgen, R. G., and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus research, 41:281-348.

Palukaitis, P., Roossinck, M.J., Dietzgen, R.G., Francki, R.I.B., 1992. Cucumber mosaic virus. Adv. Virus Res. 41, 281-348.

Palukaitis, P., Gracia- Arenal, F., 2003. Cucumoviruses. Adv. Virus Res. 62, 241-323.

Postman, J. D., Tzanetakis, I. E., and Martin, R. R. 2004. First report of Strawberry latent ring spot virus in Mentha sp. From North America. Plant Dhs. 88:907.

Qwen, J., Palukaitis, P. 1988. Characterization of Cucumber mosaic virus . Virology, 66:495-502.

Sether, D. M., DeAngelis, J. D., and Rossignol, P. A. 1991. First report of tomato spotted wilt virus in peppermint (M. pipperita). Plant Dis. 75:644.

Stone, W. J., Mink, G. I., and Bergeson, G. B. 1962. A new disease of American spearmint caused by tobacco ring spot virus. Plant Dis. Rep. 46:623-624.

Tzanetakis, I. E., Postman , J. D., and Martin, R. R. 2005. A

member of the Closteroviridaea from mint with similarities to all three genera of the family. Plant Dis. 89:654-658.

Tzanetakis, I. E., Postman, J. D., and Martin, R. R. 2005. Characterization of a Novel Member of the Family Closteroviridae from Mentha spp. Phyto, 95:1043-1047.

Tzanetakis, I. E., Postman , J. D. and Martin, R. R. 2006. Mint virus X: a novel

potexvirus associated with symptoms in variegata mint. Arch. Virol .151:43-153.

Vicchi, V., and Bellardi, M. G. 1988. The roles of weeds in the epidemiology of gladiolus viruses and MLO. Acta Hortic. 234:371-378.