Document Type: Original Article

Authors

1 Biotechnology Department, Faculty of Agriculture, Pyam e Noor University, Alborz, Iran

2 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj, Iran

Abstract

Six strain of Trichoderma (T. viride, T. harzianum, T. reesei, T. longibrachiatum, T. koningii, T. virens) were isolated and used for extracellular enzyme production. Species of Trichoderma are collected from the surrounding soil of suger beet root fields in East Iran, and held in vitro cultured PDA. TCM and TFM are used for the growth of spores and induceing the production of Cellulase. Finally the enzyme is measured at 50ºC for 60 minutes by DNS method and the samples are absorbed by spectrophotometry at wavelength 540 nm. By product of Pistachio was used to enzyme production and extracellular protein production and endo-glucanase, exoglucanase, β-glucosidase and FPase activity was investigated. T. harzianum produces high levels of endo-glucanase, exoglucanase and total cellulase, which can be further improved by controlled culture conditions. This strain can be a good candidate for obtaining cellulases from lignocellulosic by products of pistachio hull.

Keywords