Document Type: Original Article

Authors

1 MSc Student, Department of Food Science and Technology, Faculty of Agriculture, University of Urmia, Urmia, Iran

2 Associate professor, Department of Food Science and Technology, Faculty of Agriculture, University of Urmia, Urmia, Iran

3 Assistant professor, Department of Food Science and Technology, Faculty of Agriculture, University of Urmia, Urmia, Iran

Abstract

In this research the 2 sub verities of Rhizopus oligosporus was used for production of antioxidative bioactive compounds from whey protein concentrate (WPC) and apple pomace. Total phenolic compounds, free radical scavenging ability by DPPH and antioxidant protection factort were evaluated in 48 determined treatments. Effect of Incubation temperature in three levels 23, 27, 31°C, incubation time at 3,8,13 days, and Apple pomace/WPC ratio at three levels of 50:50, 70:30, and 90:10 were studied. The polyphenol content, percent of DPPH inhabitation and APF of the extracts was found to be in the range of 6.01-11.12 mg GAE/g DW, 65.8%-95.2% and 1-1.55 of samples respectively, depending on the ratio of Apple/WPC, time of incubation, type of fungi and temperature. The highest obtained efficiency was related to Rhizopus oligosporus PTCC NO.5287 and the results showed that the time of incubation had the most effect on measured parameters.

Keywords

Correia RTP, Magalhaes MMA, Macedo GR. Enriquecimento protÈico de res¥˝duos de frutas atravÈs do cultivo em meio sÛlido por leveduras S. cerevisae. In: Presented at the firsst Congresso Brasileiro de Sociedade Brasileira de Biotecnologia. S„o Paulo, SP, 6ñ8 November.
Barreto DE, Menezes TJ, Salva JG, Baldini VL, Papini RS, Sales AM. Protein enrichment of citrus wastes by solid substrate fermentation. Process Biochem 1989; 24: 167ñ71.
Zheng Z, Shetty K. Solid-state production of polygalacturonase by Lentinus edodes using fruit processing wastes. Process Biochem 2000; 35: 825ñ30.
Suarez, B., Alvarez, A. L., Garcia, Y. D., Barrio, G., Lobo, A. P., & Parra, F. (2010). Phenolic profiles, antioxidant activity and in vitro antiviral properties of apple pomace. Food Chemistry, 120(1), 339ñ342.
Toma, M., Vinatoru, M., & Mason, T. J. (2001). Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrasonic Sonochemistry, 8(2), 137ñ142.
Toma, M., Vinatoru, M., & Mason, T. J. (2001). Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrasonic Sonochemistry, 8(2), 137ñ142.
Shetty K, Labbe RG. Food borne pathogens, health and dietary phytochemicals. Asia Pac J Clin Nutr 1998; 314:270ñ6.
Vattem DA, Shetty K. Solid-state production of phenolic antioxidants from cranberry pomace by Rhizopus oligosporus. Food Biotechnol 2002; 16:189ñ210.
Wijngaard, H. H., RÄossle, C., & Brunton, N. (2009). A survey of Irish fruit and vegetable waste and byproducts as a source of polyphenolic antioxidants. Food Chemistry, 116(1), 202ñ207.
Wijngaard, H. H., RÄossle, C., & Brunton, N. (2009). A survey of Irish fruit and vegetable waste and byproducts as a source of polyphenolic antioxidants. Food Chemistry, 116(1), 202ñ207