Document Type: Original Article

Author

Young Researchers and Elite Club, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

Abstract

Reactive oxygen species cause to release of dormancy in many plants such as sunflower seeds. This study investigated in order to evaluation role of reactive oxygen species germination and lipid proxidation in sunflower seeds. This study was performed in two separate experiments, each in a completely randomized design with factorial design with four replications.  In both experiments, uses from dormant and non dormant seeds of sunflower. It also applies of treatments Methylviologen and Cyanide in dormant seeds which are the producers of reactive oxygen species. Finally, germination lipid proxidation were evaluated as well. The results showed that the main reason for release of sunflower seeds dormancy is production of reactive oxygen species is an acceptable level so that seed germination of dormant seeds which was treated with Methylviologen and Cyanide was more than dormant control seeds and was similar to non dormant seeds. The amount of lipid peroxidation product malondialdehyde in dormant seeds was less than non dormant seeds and seeds treated with Methylviologen and Cyanide.

Keywords

Alyaree, H., Shekari, F. (2000). Oil seeds, agronomy and physiology. Amidi press. 182 pp.
Dalton TP, Shertzer HG, Puga A. Regulation of gene expression by reactive oxygen, 1999, Annu Rev Pharmacol Toxicol 39: 67ñ101,.
Elstner, E.F. and Heupel, A. (1976) Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Anal. Biochem. 70, 616ñ620.
Gay, C., Corbineau, F. and Coà me, D. (1991) Effects of temperature and oxygen on seed germination and seedling growth in sunflower (Helianthus annuus L.). Environ. Exp. Bot. 31, 193ñ200.
Giannopolitis. C.N. and Ries S.K. (1977). Superoxide dismutases i. Occurrence in higher plants. Plant Physiology. 59: 309-314.
Gill, S.S., Tuteja, N., Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants, 2010, Plant Physiology and Biochemistry, 48: 909-930.
Grant, J.J., Loake, G.J. Role of reactive oxygen intermediates and cognate redox signaling in disease resistance, 2000. Plant Physiology 124, 21ñ30.
Heath, R.L., and Packer, L. 1968. Photoperoxidation in isolated choloroplast.1. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 12: 189-198.
Khaje poor, M.R. 2004. Industrial plants. Jihad of Isfahan Technology press. 564pp.
Leubner-Metzger G. Beta-1,3-glucanase gene expression in low-hydrated seeds as a mechanism for dormancy release during tobacco after-ripening. 2005, Plant J; 41:133-45.
Liszkay A, van der Zalm E, Schopfer P. Production of reactive oxygen intermediates (O2.-, H2O2, and. OH) by maize roots and their role in wall loosening and elongation growth. 2004, Plant Physiol; 136:3114-23.
Luck, H. 1962. Methods of enzymatic analysis. E.B. By Bergmeyer (1th edition), Verlag chemie weinheim. Pp:885-894.
McDonald, M.B. Seed deterioration: physiology, repair and assessment. 1999. Seed Science and Technology. 27:177ñ237.
Moller, I.M. ëPlant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species, 2001, Annual Review of Plant Physiology and Plant Molecular Biology 52, 561ñ591.
Oracz, Krystyna,, Hayat El-Maarouf Bouteau, Jill M. Farrant, Keren Cooper, Maya Belghazi, Claudette Job, Dominique Job, Franc¸oise Corbineau and Christophe Bailly. 2007. ROS production and protein oxidation as a novel mechanismfor seed dormancy alleviation. The Plant Journal. 217: 1-14.