Document Type: Original Article

Authors

1 Department of horticulture science, Islamic Azad University, Miyaeh branch, Miyaneh, Iran

2 Assistant professor, Department of agriculture, Payame Noor University, Tehran, Iran

3 Young Researchers and Elite Club, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

4 Young Researchers and Elite Club, Science and Research Branch, Ardabil, Iran

Abstract

Essential oils from aerial parts of Mentha spicata, was obtained by steam destillation using a Clevenger-type system. These oils were screened for antibacterial and anti-Candida albicans activity using bioautographic method. A fiel experiments was laid out in agricultural research farm, city Namin, Ardabil province, Iran in 2013. Experiment was conducted in a randomized complete block with seven treatments and three replication. Foliar spray treatments urea solution at a rate of 1, 2 and 3% (in two stages, the first stage a month after transplanting when they had reached 10 cm in height to 20 days after the first stage and second stage ), soil application of vermicompost fertilizer at a rate of 5, 10 and 15 tons per hectare and control. Chlorophyll in two phases, one week after the first treatment and harvest samples were measured by chlorophyll meter SPAD manually. Essential oil extracted from the leaves and twigs using 50 g samples of each treated flower with water distillation by Clevenger apparatus was performed. Results showed that the effect of experimental treatments on essential oil and chlorophyll were significant at the one percent level of probability. maximum chlorophyll index foliar urea treatment 3 percent and the lowest was observed in the control. Most essential oils (114.3cc per square meter) in 2% foliar urea treatment and the lowest (36.9cc per square meter) was obtained in control treatment. An increase of over 300% in performance is seen to be essential.

Keywords

Adam, K.; Sivropoulou, A.; Kokkini, S.; Lanaras, T.; Arsenakis, M. Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa Essential Oils against Human Pathogenic Fungi. J. Agric. Food Chem., 46:1739- 1745, 1998.
Anwar, M., Patra, D.D., Chand, S., Alpesh, K., Naqvi, A.، A. khanuja, S.P.S., 2005. Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation, and oil quality of French basil Communications in Soil Science and plant Analysis, 36 (13-14): 1737-1746.
Atiyeh, R.M., Edwards, C.A., Subler, S., Metzger, J.D., 2001. Pig manure vermicompost as a component of a horticultural bedding plant medium: effects on physicochemical properties and plant growth. Bioresource Thechnology 78 (11-20).
Cecanho, R.; Koo, H.; Rosalen, P.L.J.A.; Park, Y.K.; Cury J.Á. Efeito do extrato hidroetanólico de Mikania laevigata sobre o crescimento bacteriano e a produção de glucamos por estreptococcus do grupo mutans. Anais da XIV Reunião Anual da FESBE, 14:290, 1999.
Charles, D.J.; Simon, J.E. Comparison of extraction methods for the rapid determination of essential oil content and composition of basil. J. Am. Hort. Sci., 115:458-462, 1990.
Galli, A.; Franzetti, L.; Briguglio, D. Attività antimicrobica in vitro di oli essenziali ed estratti di spezie di uso alimentare. Ind. Alim., 463-466, 1985.
Guillén, M.D.; Cabo, N.; Burillo, J. Characterisation of the essential oils of some cultivated aromatic Plants of industrial interest. J. Sci. Food Agric., 70:359-363, 1996.
Kanna, P., Saravanan, A., Krishnakumar, S., Natarajan, S.K. 2005. Biological Properties of Soil as Influenced by Different Organic Manures. Research Journal of Agriculture and Biological Sciences 1(2):
181-183.
Omidbeygi, R. 2011. Processing of medicinal plants. V 2. 289pp.