Document Type: Original Article


Department of Horticulture, College of Agriculture, Roudehen Branch, Islamic Azad University (I A U), Roudehen, Iran


Studies had shown that oxygenated compounds are important in food products. It seems that extraction methods had a profound influence on this factor. The goal of the present study is to investigate on flavor components of Murcott mandarin obtained using cold-press and hydro distillation. In the last week of January 2012, at least 50 mature fruit were collected from many parts of the same trees. Peel components were extracted using cold-press and hydro distillation method. Then all analyzed using GC and GC-MS. Data were analyzed using one-way analysis of variance (ANOVA) and Duncan’s multiple range tests. The amount of aldehydes ranged from 0.36% to 0.66%. Between two methods examined, cold-press showed the highest content of aldehydes. As a result of our study, can be concluded that the extraction methods used can influence the quantity of oxygenated compounds present in the oil. The application of Cold-press method can cause a lesser damage to thermal-sensitive molecules, so can be a good technique to recovery of Citrus compounds in large industrial scale.


Adams, R.P. (2001). Identification of essential oil components by gas chromatography / mass spectrometry, Allured Publishing Corporation, Carol Stream, Illinois, USA.
Babazadeh-Darjazi, B., Rustaiyan, A., Taghizad, R and Golein, B. (2011a) . A study on oxygenated constituents percentage existed in page mandarine peel oil during a special season. J Med Plant, 4 (2):87-93.
Babazadeh- Darjazi, B. (2011b). Comparison of volatile components of flower, leaf, peel and juice of ëPageí mandarin. Afr J Biotechnol, 10 (51):10437-10446.
Babazadeh- Darjazi, B. (2013a). A study on peel volatile components and juice quality parameters of two tangor (Citrus reticulata × Citrus sinensis) scions. Inter J Agri Biosci, 2(6): 371-376.
Babazadeh- Darjazi, B. (2013b). The effect of two Citrus (Citrus sp.) scions on peel components and juice quality parameters. Intl J Agri Crop Sci, 6 (15):1079-1087.
Babazadeh- Darjazi, B. (2014a). Comparison of Peel Components of Sweet Orange (Citrus sinensis) Obtained using Cold-press and Hydro Distillation Method. Inter J Agri Biosci, 3(1): 13-17.
Babazadeh- Darjazi, B. (2014b). Comparison of peel components of Pummelo (Citrus grandis) obtained using cold-press and hydrodistillation method. J Life Sci Biomed, 4(2): 71-77.
Bousbia, N., Vian M.A., Ferhat M.A., Meklati B, Y and Chemat, F. (2009). A new process for extraction of essential oil from Citrus peels: Microwave hydrodiffusion and gravity. J Food Engine, 90(3):409-413.
Braddock, R.J. (1940). Hand book of Citrus by products and processing technology, Wiley, USA.
Buettner, A., Mestres, M., Fischer, A., Guasch, J and Schieberie, P. (2003). Evaluation of the most odoractive compounds in the peel oil of clementines (Citrus reticulate Blanco cv. Clementine). Eur Food Res Technol, 216: 11-14.
FAO (2012). Statistical Database. Available from:
otouhi, R and Fattahi, J. (2007). Citrus growing in Iran, 2nd edn. Gilan University, Rasht.
Gaspar, F and Leeke, G. (2004). Comparison between compressed Co2 extracts and hydrodistilled essential oil. J Essent Oil Res, 16: 64-68.
Gontaru, L. (2009). Stabilization of sensitive substances by antioxidants from summer savory and encapsulation. PhD of science thesis. Bochum University, pp.13.
Habashi, M., Mirza, M., Mostofi, Y and Jaimand, K. (2009). Identification and comparison of the essential oil components from the peel of citron (Citrus medica L.) by using two extraction methods (hydrodistillation and cold press). Iranian J Med Aroma Plants, 24(4): 428-436.
Handa, S.S.(2008). An overview of extraction techniques for medicinal and aromatic plants. In: Extraction technologies for medicinal and aromatic plants. Edited by Handa, S.S., Khanuja, S.P.S., Longo, G and Rakesh, D.D. United Nations Industrial Development Organization and the International
Centre for Science and High Technology,Padriciano, Italy.41-44.
Lawrence, B.M. (1995). The isolation of aromatic materials from natural plant products. In: A manual on the essential oil industry. Edited by Silva, T.D. Nations Industrial Development Organization (UNIDO),
Vienna, Austria. pp.83.
McLafferty, F.W and Stauffer, D.B. (1991). The important peak index of the registry of mass spectral data. Wiley, New York. USA.
Menichini, F., Tundis, R., Bonesi, M. , Cindio, B.d. , Loizzo, M.R. , Conforti, F. , Statti, G.A. , Menabeni, R., Bettini, R and Menichini, F.(2011). Chemical composition and bioactivity of Citrus medica L. cv. Diamante essential oil obtained by hydrodistillation, cold-pressing and supercritical carbon
dioxide extraction. Nat Pro Res, 25(8): 789-799.
Porto, C.D and Decorti, D. (2009). Ultrasound-assisted extraction coupled with under vacuum distillation of flavor compounds from spearmint (carvone-rich) plants: Comparison with conventional hydrodistillation.Ultrason Sonochem, 16: 795-799.
Salem, A. (2003). Extraction and identification of essential oil components of the peel, leafand flower of tangerineîCitrus nobilis loureior var deliciosa swingleî cultivated at the north of Iran. Master of Science thesis, Islamic Azad University, Pharmaceutical sciences branch.
Scora, R.W., Esen, A and Kumamoto, J. (1976). Distribution of essential oils in leaf tissue of an F2 population of Citrus. Euphytica, 25: 201-209.
Swisher, H.E and Swisher, L.H. (1977). Specialty Citrus products. In: Citrus science and technology.
Edited by Nagy, S., Shaw, P and Veldhuis, M.K. The AVI Publishing Company, Westport. 291-299.
Verzera, A., Trozzi, A., Gazea, F., Cicciarello, G and Cotroneo, A.(2003). Effect of rootstock on the composition of bergamot (Citrus bergamia Risso et Poiteau) essential oil. J Agric Food Chem, 51: 206-210.