Document Type: Original Article

Authors

1 Graduated from veterinary faculty of Islamic Azad University, Shahrekord, Iran

2 Food hygiene department, veterinary faculty of Islamic Azad University, Shahrekord, Iran

Abstract

Clostridium difficileisa binding warm-positive rod and anaerobic organism, which was first described in 1935 as Bacillus difficile. Epidemiology of CDI has changed considerably on 2000. Occurrence and severity of the disease  including ulcerative colitis(inflammation of the colon), colectomy(colon section) and mortality had increased. Recent studies have reported Clostridium difficile prevalence in animal foods ,act as a source of infection for humans, its source can regarded as a contaminate food, infecting dose and the relationship between food contamination and disease. Clostridium difficile causes critical  disease in humans. So, this study by the aim of evaluation of  possible contamination of turkey and ostrich meat to the pathogen was designed and implemented. To conduct this study, a total of 240 samples of raw ostrich meat (n=120) and Turkey (n=120) purchased randomly of meat stores of Isfahan- Iran and then transferred to the Laboratory of Infectious and Tropical Diseases Research Center, Isfahan University, evaluated regarding existence of Clostridium difficile by enrichment method and then culture on CDMN medium under anaerobic conditions and biochemical tests and observations of warm staining. In this study, a total of 240 samples, 25 samples(10/41%) were carrier of C.difficile. of  11 of 120 ostrich meat samples(9/16%) and 14 samples out of 120 samples of turkey meat (11/66%) Clostridium difficile was isolated. Clostridium difficile in turkey and ostrich meat sold in stores in Isfahan - Iran is isolated and thus a potential risk to humans especially through meat consumption are microorganisms

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Arroyo LG, Weese JS, Staempfli HR. 2004. Experimental Clostridiu m difficile enter colitis in foals. Journal of Veterinary Internal Medicine 18, 734 –738.
Bakri MM, Brown DJ, Butcher JP, Sutherland AD. 2009. Clostridium difficile in ready-to-eat salads, Scotland. Emerging Infectious Diseases 15(5), 817–8.
Barbut F, Richard A, Hamadi K, Chomette V. 1997. Burghoffer B, Petit JC, Epidemiology of recurrences or re-infections of Clostridium difficile –associated diarrhoea. Journal of Clinical Microbiology 38, 2386 –2388.
Bayati Zadeh. J, Moradi kor. Z. (2013). Ruminal protein degradation and estimation of rumen microbial protein production. International journal of Advanced Biological and Biomedical Research (IJABBR) . 1(8):914-921.
Braun V, Hundsberger T, Leukel P, Sauerborn M, von Eichel-Streiber C. 1996. Definition of the single integration site of the pathogenicity locus in Clostridium difficile. Gene 181(1–2), 29–38.
Ebrahimpour. M., Kiyani. V., Mohammadhasan. H. (2013). Investigation acute toxicity some of heavy metals at different water hardness. International journal of Advanced Biological and Biomedical Research (IJABBR) . 1(2):134-142.
Goncalves C, Decre D, Barbut F, Burghoffer B, Petit JC. 2004. Prevalence and characterization of a binary toxin (actin-specific ADP-ribosyltransferase) from Clostridium difficile. Journal of Clinical Microbiology 42, 1933–1939.
Hall I, O’Toole E. 1935. Intestinal flora in newborn infants with a description of a new pathogenic anaerobe, Bacillus difficilis. Archives of Pediatrics & Adolescent Medicine 49, 390–402.
Harvey RB, Norman KN, Andrews K, Hume M.E, Scanlan C.M, Callaway T.R, Anderson RC, Nisbet DJ. 2011. Clostridium difficile in Poultry and Poultry Meat. Foodborne Pathogens and Disease 8(12), 1321-3.
Indra A, Lassnig H, Baliko N,Much P, Fiedler A, Huhulescu S. 2009. Clostridium difficile: a new zoonotic agent. Wiener klinische Wochenschrift 121(3–4), 91–5.
Jump RL, Pultz MJ, Donskey CJ. 2007. Vegetative Clostridium difficile survives in roomair on moist surfaces and in gastric contents with reduced acidity: a potential mechanism to explain the association between protons pump inhibitors and C.difficile-associated diarrhea. Antimicrob Agents Chemother
51(8), 2883-7.
Loo VG, Poirier L, Miller MA, Oughton M, Libman MD, Michaud S. 2005. A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. The New England Journal of Medicine 353(23), 2442–9.
Marks SL, Kather EJ, Kass PH, Melli AC. 2002. Genotypic and phenotypic characterization of Clostridium perfringens and Clostridium difficile in diarrheic and healthy dogs. Journal of Veterinary Internal Medicine 16, 533– 540.
Martin H, Willey B, Low DE, Staempfli HR, McGeer A, Boerlin P. 2008. Characterization of Clostridium difficile strains isolated from patients in Ontario, Canada, from 2004 to 2006. Journal of Clinical Microbiology 46, 2999–3004.
McDonald LC, Owings M, Jernigan D.B. 2006. Clostridium difficile infection in patients discharged from US short-stay hospitals, 1996-2003. Emerging Infectious Diseases 12(3), 409–15.
McEllistrem MC., Carman RJ, Gerding DN, Genheimer CW, Zheng L. 2005. A hospital outbreak of Clostridium difficile disease associated with isolates carrying binary toxin genes. Clinical Infect Disease 40(2), 265–72.
Muto C.A, Pokrywka M, Shutt K, Mendelsohn AB, Nouri K, Posey K, Roberts T, Croyle K, Krystofiak S, Patel-Brown S, Pasculle A.W, Paterson DL, Saul M, Harrison LH. 2005. A large outbreak of Clostridium difficile associated disease with an unexpected proportion of deaths and colectomies at a teaching hospital following increased fluoroquinolone use. Infect Control Hosp
Epidemiol 26(3), 273–80.
Paltansing S, van den Berg RJ, Guseinova RA, Visser CE, van der Vorm ER, Kuijper EJ. 2007. Characteristics and incidence of Clostridium difficile- associated disease in The Netherlands, 2005. Clinical Microbiology and Infection 13, 1058–1064.
Pituch H, Rupnik M, Obuch-Woszczatynski P,Grubesic A, Meisel-Mikolajczyk F, Luczak M. 2005. Detection of binary-toxin genes (cdtA and cdtB) among Clostridium difficile strains isolated from patients with C. difficile-associated diarrhoea (CDAD) in Poland. Journal of Medical Microbiology 54,143–147.
Rodriguez-Palacios A, Reid-Smith RJ, Staempfli HR,Daignault D, Janecko N, Avery BP. 2009. Possible seasonality of Clostridium difficile in retail meat, Canada. Emerging Infectious Diseases 15(5), 802–5.
Rodriguez-Palacios A, Staempfli HR, Duffield T, Weese JS. 2007. Clostridium difficile in retail ground meat, Canada. Emerging Infectious Diseases 13(3), 485–7.
Rupnik M, Dupuy B, Fair weather NF, Gerding DN, Johnson S. 2005. Revised nomenclature of Clostridium difficile toxins and associated genes. Journal of Medical Microbiology 54, 113–117.
Sharifinia. M., Ramezanpour. Z., Imanpour. J., Mahmoudifard. A., Rahmani. T. (2013). Water quality assessment of the Zarivar Lake using physico-chemical parameters and NSF- WQI indicator, Kurdistan Province-Iran. International journal of Advanced Biological and Biomedical Research (IJABBR) .
1(3):302-312.
Simango Mwakurudza. 2008. Clostridium difficile in broiler chickens sold at market places in Zimbabwe and their antimicrobial susceptibility. International Journal of Food Microbiology 124, 268 –270.
Soehn F, Wagenknecht-Wiesner A, Leukel P, Kohl M, Weidmann M, von Eichel-Streiber C, Braun V. 1998. Genetic rearrangements in the pathogenicity locus of Clostridium difficile strain 8864– implications for transcription, expression and enzymatic activity of toxins A and B. Mol Gen Genet 258(3), 222–32.
Songer JG, Trinh HT, Killgore G.E, Thompson AD, McDonald LC, Limbago BM. 2009. Clostridium difficile in retail meat products, USA, 2007. Emerging Infectious Diseases 15(5), 819–21.
Songer JG, Post KW, Larson DJ, Jost BH, Glock RD. 2000. Infection of neonatal swine with Clostridium difficile. Swine Health and Production 8, 185–189.
Weese JS, Avery B, Rouss eau J, Reid-Smith R. 2009. 19th European Con-gress of Clinical Microbiology and Infectious Diseases; 2037 p.