Document Type : Original Article
Authors
- Homayoun Banani Rad 1
- Jamal Fayazi 1
- Cyrus Amirinia 2
- Hedayatolah Roshanfekr 1
- Hamidreza Seydabadi 3
1 Department of Animal Science, Ramin Agriculture and Natural Resources University, Khouzestan, Iran
2 Department of Biotechnology, Animal Science Research Institute, Iran
3 Department of Biotechnology, Animal Science Research Institute,Iran
Abstract
Pedigree information and phenotypic records are the key features in animal genetics and breeding. Otherwise best methods for genetic evaluation and estimation of parameters will be tending to no correct results. This study was aimed to investigate and verify pedigree relations among 76 individuals who were genotyped for 3 fluorescent labeled microsatellite markers picked up from FAO-ISAG joint recommended list as one multiplex PCR set. 11 sires, 11offspring and 11 dams with 43 unknown samples were analyzed. Based on exact allele sizes, 33 alleles were observed within the studied population. The mean number of alleles per locus was 11. Polymorphism information content (PIC) values ranged from 0.3257 to 0.8043 with the mean value of 0.6213. The most informative loci were :BM2113 (16alleles,PIC=0.7338), BM1818(3alleles,PIC=0.3257).The indices of genetic variability within population and polymorphism of loci including average heterozygosis, the number of observed and effective alleles, polymorphic information content (PIC) and so on showed that the studied khouzestan buffaloes population and used markers have still a reliable diversity and polymorphism level, respectively. Also, combined probability of exclusion (PE) values obtained per all loci in both parentage and identification analysis was 0.9381 and 0/4483thatindicate the high efficiency of studied marker set for parentage and identification test in this population
Keywords
Bonin A., Belle main E., B r o n k e n -Eideen P., Pompanon F., BrochmannC., Ta berle t P. (2004). How to track and assess genotyping errors in population genetics studies. Mol. Ecol., 13 (11): 3261–3273.
Carolin o I., Sousa C.O., Ferreira S., Carolin o N., Silva F.S., Gam a L.T. (2009). Implementation of a parentage control system in Portuguese beef cattle with a panel of microsatellite markers. Genet. Mol. Biol., 32 (2): 306–311.
Dadpasand M., Miraei - Ashtiani S.R., Moradi Shahrebabak M., VaezTorshizi R. (2008). Impact of conformation traits on functional longevity of Holstein cattle of Iran assessed bya Weibull proportional hazards model. Livest Sci., 118: (3) 204–211.
Kalinowski S.T., Taper M.L., MarshalT.C. (2007). Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol. Ecol., 16 (5): 1099–1106.
Kios D., Marle - Koster E.V., VisserC. (2011). Application of DNA markers in parentage verification of Boran cattle in Kenya. Tropical Animal Health and Production. Trop. Anim. Health Pro., 44 (3): 471–476.
Miller S.A., DykesD.D.,Polesky H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res., 16 (3), p. 1215.
Mitchels on K.R., Cheng J. (2001). Capillary Electrophoresis of Nucleic Acids (Volume I: Introduction to the Capillary Electrophoresis of Nucleic Acids). Humana Press, Totowa, New Jersey, 484 pp.
MunozP., Resende M., Peter G., HuberD., Kirst M., Quesada T. (2011). Effect of BLUP prediction on genomic selection: practical considerations to achieve greater accuracy in genomic selection. Tree Biotechnology Conference: From Genomes to Integration and Delivery, Brazil, 49.
Ozakan E., Soysa l M.I., Ozder M., Koban E., Sahin O., Togan I. (2009). Evaluation of parentage testing in the Turkish Holstein population based on 12 microsatellite loci. Livest Sci., 124 (1): 101–106.
Putnova L., VrtkovaI., Srubarova P., StehlikL. (2011). Utilization of a 17 microsatellites set for bovine traceability in Czech cattle populations. IJAS., 1 (1): 33–38
Rehout V., Hradecka E., Citek J. (2006). Evaluation of parentage testing in the Czech population of Holstein cattle. Czech. J. Anim. Sci., 51 (12): 503–509.
Van VleckL.D., (1970 b). Misidentification and sire evaluation. J. Dairy Sci., 53 (12): 1697–1702.
VanVleck L.D. (1970 a). Misidentification in estimating the paternal sib correlation. J. Dairy Sci., 53(10): 1469–1474
Zhang Y., Wang Y., SunD., YuY., ZhangY. (2010). Validation of 17 microsatellite markers for parentage verification and identity test in Chinese Holstein cattle. Asian Austral. J. Anim., 23 (4):425–429.
)