Document Type: Original Article

Authors

Department of Animal Science, Faculty of Animal Science and Food Industries, Khuzestan Ramin Agricultural and Natural Resources University, Ahwaz, Iran

Abstract

Objective: Identity the genetic aspects and major gene influence on energy balance, milk production, fertility, food safety and consumer are the recent interests of genetic and breeding researchers. Methods: Najdi Cattle is the most prominent breeds in Khuzestan province. To do this plan in Shoushtar Najdi Cattle Station, blood samples were taken from 15 Najdi Cattles. DNA was extracted from whole blood using the method of Boom et al and polymerase chain reaction took for amplification of two fragments 544 bp and 566 bp. After ensuring the accuracy of the duplicated sequences on 1% agarose gel, sequencing was performed and the Leptin gene promoter components pointed out in Najdi Cattle. In this study, the six mutations identified in the first fragment 566 bp. In these mutations, 2 types of them were point mutations including Transition and Transversion, and 4 mutations were Frame shift which included Deletion (3kinds) and Insertion (1kind). In the second fragment 544 bp, 2 point mutations (transition and Transversion) and 1 Frame shift mutation (deletion) were appeared, in the Leptin gene pro-motor region. Also, in this study, the sequences related to each sample was compared in the two fragments in the aspect of similarity with other documented sequences, that BLAST results showed that high percentage of similarity. Results: According to the obtained results, it could be said that the sequence of leptin gene of Najdi cattle in Khuzestan province is similar to other documented sequences and there are a high-similarity percentage between current samples’ sequences and those documented in global information bank.

Keywords

Agarwal, R., Rout, P.K., Singh, S.K., 2008. Leptin: A bio molecule for enhancing livestock productivity. Indian. J. Biotechnol. 8, 169-176.
Barb, C.R., Hausman, G.J., Housecknecht, K.L., 2001. Biology of leptin in the pig. Domest. Anim. Endocrinol. 21, 297-317.
Boom, R., Sol. C.J.A., Salimans, M.M.M., Jansen, C.L., Wertheim-van Dillen, P.M.E., van Der Noordaa, J., 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495-503.

Buchanan, F.C., Fitzsimmons, C.J., Van Kessel, A.G., Thue, T.D., Winkelman, D.C., Schmutz, S.M., 2002. Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet. Sel.
Evol. 34, 105-116. 
Cunningham, M.J., Clifton, D.K., Steiner, R.A., 1999. Leptins actions on the reproductive axis: perspectives and mechanisms. Bio. Reprod. 60, 216-222.
Galehdari, H., Foroghmand, A., Roshanfekr, H. A., Nazari, M., 2006. Genetic engineering Comprehensive. Golhaye Behesht Publication, Ahvaz, Iran (in Persian).
Javanmard, A., Khaledi, K., Asadzadeh, N., Solimanifarjam, A.R., 2010. Detection of polymorphisms in the bovine leptin (LEP) gene: association of single nucleotide polymorphism with breeding value of milk traits in
Iranian Holstein Cattle. J. Mol. Gene. 2, 10–14.
Lagonigro, R., Wiener, P., Pilla, F., Woolliams, J.A.,
Williams, J.L., 2003. A new mutation in the coding region of the bovine leptin gene associated with feed intake. Anim. Gene. 34, 371- 374.
Liefers, S.C., Pas, M.F.W., Veerkamp, R.F., Van der Lende, T., 2002. Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. J. Dairy. Sci. 85: 227–238.
T., Melonek, J., Switonski, M., Strabel, T., 2004. Short Communication: Effect of Leptin Gene Polymorphisms on Breeding Value for Milk Production Traits. J. Dairy. Sci. 87, 3925-3927
Meier, U., Gressner, A.M., 2004. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem. 50, 1511-1525.
Nobari, K., Ghazanfari, S., Nassiry, M.R., Tahmoorespur, M., Jorjani E., 2010. Relationship between leptin gene polymorphism with economical traits in Iranian Sistani and Brown Swiss Cows. J. Anim. Vet. Advan. 9, 2807-2810.
Taniguchi, Y., Itoh, T., Yamada, T., Sasaki Y., 2002. Genomic Structure and Promoter Analysis of the Bovine Leptin Gene. Iubmb Life. 53, 131-135.
Terence Austen, B., 2011. Gene cloning and DNA analysis an introduction: The house of biotechnology publisher. P, 440.
Zhang, F., Basinski, M.B., Beals, J.M., Briggs ,S.L., Churgay, L.M., (1997). Crystal structure of obese protein leptinEloo. Nature. 387, 206-209.
Zhou, H., Hickford, J.G., Gong H., 2009. Identification of allelic polymorphism in the ovine leptin gene. Mol. Biotechnol. 41: 22 – 25.