Document Type: Original Article

Authors

1 Department of Biological Sciences, Federal University Otuoke, Nigeria

2 Department of Animal and Environmental Biology, University of Benin, Benin City, Nigeria

Abstract

Objective: The toxic effects of sublethal concentrations of endosulfan on Oreochromis niloticus was examined using bioaccumulation and histopathology studies of the liver as toxicological endpoint.  Methods: The Organization for Economic Cooperation and Development #407 recommended static renewal bioassay was employed during the test .The test fish were exposed to sub-lethal concentrations separately for a period of 7, 14, 21, and 28 days. At the end of each exposure period the liver tissues were isolated and analyzed using high performance liquid chromatography for bioaccumulation study, while alterations observed in exposed fish were determined in the laboratory by light microscopy using standard technique of Haematoxylin and Eosin staining. Results: No mortalities occurred in any group during the test .The severity of the histological alterations observed in the liver of the treated fish reflects the concentrations of the toxicant, level of depuration and exposure durations .An indication that the regulating mechanisms of the liver has been overwhelmed resulting in the structural damages .The findings showed that the body could be liberated of contaminants after longer exposure period however, the effects on the body’s tissues remain lethal.

Keywords

Al- Yousuf MH, El-Shahawi MS, Al-Ghais SM (2000) Trace metals in liver, skin and muscle of Lethrinus lentjan fish species in relation to
body length and sex. Sci. Total Environ 256: 87-94. CrossRef | Direct Link |
APHA (2005) (American Public Health Association): Standard Methods for the Examination of Water and Wastewater. Washington DC: APHA, AWWA,
WEF
Arnot J, Gobas, F (2004) A food web bioaccumulation model for organic chemicals in aquatic ecosystems Environ Toxicol and Chem 23, 2343-
2355 National Oceanic and Atmospheric Administration Technical Memorandum, NMFS F/SEC-11
Atamanalp M, Sisman T, Geyikoglu F, Topal A (2008) The histopathological effects of Copper sulphate on rainbow trout liver (Oncorhynchus
mykiss) J Fish Aquat Sci 12: 67-89
Bancroft JD, Cook HC (1994) Manual of histological techniques and their diagnostic application Churchill Livingstone, London: 289ñ305

Manual of histological echniques and their diagnostic application Churchill Livingstone, New York
Bernet DH, Schmidt W, Meier P, Burkhardt H, Wahli T (1999) Histopathology in fish: Proposal for a protocol to assess aquatic pollution J Fish Disease, 22:25-34
Cengiz E, Unlu E (2006) Sublethal effects of commercial deltamethrin on the structure of the gill, liver and gut tissues of mosquitofish, Gambusia
affinis: A microscopic study Environ ToxicolPharmacolology 21: 246-253
Daesik P, Minor MD, Proper, CR (2004) Toxic response of
endosulfan to breeding and non breeding female mosquito fish J Environ Bio 25(2) 119-124
Debruyn A , Ikonomou M, Gobas F (2004) Magnification and Toxicity of PCBs, PCDDs, and PCDFs in Upriver-Migrating Pacific Salmon Environ
Sci Technol 38: 6217-6224 
Eller LL (1971) Histopathological Lesions in Cutthroat Trout Salmo clarki Exposed chronically To The Insecticide Endrin Am J Pathol 64, 321ñ328
Ezemonye LIN , Ikpesu T O,Tongo I (2010) Distribution of endosulfan in water,sediment And fish samples from Warri River, Niger Delta Nigeria
Africa Journal of ecology 16(4) 67 ñ80
Feeley MM, Jordan SA (1998) Dietary and tissue residue analysis and contaminant intake estimationsin rats consuming diets composed of Great
Lakes salmon: a multigeneration study Regul Toxicol Pharmacol 27: 8-17
Fisher RA (1950) Statistical methods for research
workers 11 th edition Oliver and Boyd, London 75 pp
Fontainhas-Fernandes AA (1998) Tilapia production In: Reis-Henriques MA (ed) Aquaculture
handbook Academic Press, California pp 135ñ150
Ghousia B, Shanta V, Nageswara S P, Sajid H (1994) Study of dimethoate bio -accumulation in liver and muscle tissues of Clarias batrachus and its
elimination following cessation of exposure Pest Science 40, 201-205 1994
Gill TS, Pant JJ1(988) Gill, Liver, and Kidney Lesions Associated with Experimental Exposures to Carbaryl and Dimethoate in the Fish (Puntius conchonius) Environ Control Toxicology 41, 71ñ78
Gill T J, Pande J, Tewari H (1991) Effects of Endosulfan on the Blood and Organ Chemistry of Freshwater Fish (Barbus conchonius Ham) Ecotoxicol Environ Safety 21, 80ñ91
Glenn S I, Gandolfi J (1986) Biotransformation of Toxicants In: The Basic Science of poisons Curtis D Klaassen, Mary O Amdur, John Doull
(eds) 3rd edition Macmillan Publishing Company, New York: pp 64-98
GÛralczyk K, StruciÒski P (1996)  persistent organochlorine compounds Accumulation of
on the respective levels of trophic pyramid Ecol Techn, 4:
14-16
Guzelian P S, Vranian JJ, Boylan WJ,Blanke RV (1980) Liver structure and function in patientsí posioned with chlordecone (Kepone) Gastroenterology 78: 206-2131980
Hughes GM, Perry SF (1976) Morphometric study of trout gills: a light microscopic method for the evaluation of pollutant action Journal of Experimental Biology 63: 447-460
International Sorbent Technology (IST) (1995) A Guide to Method Development in Solid Phase Extraction of Water Samples Using IsoluteÆ ENV+ôSPE Columns International Sorbent Technology
Jimener B D,Stegman S S (1990) American Fisheries Society Symposium pp 867-879
Latuszynska JS, Luty JH, Przylepa E, Tochman A, Obuchowska D, Korczak E (1999) Studies of toxicity of dermally-absorbed nurelled D 550 EC preparations Ann Agri Environ Medicine 6: 151-159
Liao CY, Fu j, Shi J, Zhou Q, Yuan C, Jiang B (2006) Methylmercury accumulation, histopathology effects, and cholinesterase activity alterations in medaka (Oryzia latipes) following sublethal exposure to methylmercury
chloride Environ Toxicolo Pharmacol, 22, 225 -233
OECD (1992) Guideline for the testing of chemicals: Fish, acute toxicity test, No 203
OECD (1995) Guideline for the Testing of Chemical 407, ëëRepeated Dose 28- day Oral Toxicity Study in Rodentsíí 1997a adopted on 27th July
Palaniappan PM, Karthikeyan S (2009) Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater
fish Cirrhinus mrigala individually and in binary solutions with nickel
Popp JA,Cattley RC (1991) Hepatobillary system In: Handbook of Toxicologic pathology (Haschek, WM and Rousseaux, CG eds) pp: 279-314 San Diego, Academic Press Inc
Rajendran N,Venugopalan V (1991) Bioconcentration of Endosulfan in different body tissues of estuarine organisms under sublethal exposure Bull Environ Contam Toxicol 46 (1),151- 158
Safe S (2003) Toxicology and Risk Assessment of POPs In: Fiedler H Editor Handbook of Environmental Chemistry Vol 3 part O Springer-Verlag,
Berlin Heidelberg: pp 223-235
Schinas V, Leotsinidis M, Alexopoulos A, Tsapanos V, Kondakis G (2000) Organochlorine pesticide residues in human breast milk from southwest Greece: associations with weekly food consumption patterns of mothers Arch
Environ Health, 55: 411-417
Van der Oost R, Beber J, Vermeulen N P E (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review Environmental
Toxicology and Pharmacology 13: 57-149