Document Type: Original Article

Authors

1 Department of Plant Protection, Faculty of Agriculture, University of Birjand., Birjand, Iran

2 Department of Plant Protection, Faculty of Agriculture, University of Birjand, Birjand, Iran

3 Department of Plant Protection, Faculty of Agriculture, University of Bu Ali Sina , Hamedan, Iran

Abstract

Objective: The genus Trichoderma comprises a great number of fungal strains that act as biological control agents, the antagonistic properties of which are based on the activation of multiple mechanisms. Methods: Trichoderma strains exert biocontrol against fungal phytopathogens either indirectly, by competing for nutrients and space, modifying the environmental conditions, or promoting plant growth and plant defensive mechanisms and antibiosis, or directly, by mechanisms such as mycoparasitism. In order for identification of Trichoderma species, sampling was performed from plant tissues and soil samples of medicinal plants in the South Khorasan province during 2012-2114 years. Several isolates of Trichoderma were isolated from soil and tissue samples using the Trichoderma selective medium. Results: Isolates were identified based on macroscopic and microscopic morphological characteristics such as a growth and shape of the colony, type of conidiophore formation, shape and size of phialide and conidium on CMD and MEA and using valid Trichoderma keys. So far, four species were identified harzianum, crassum, brevicompactum, and virens spices. T. harzianum was the most frequent species among isolates and isolated from Russian olive, Camelthorns, Jujube, Barberry and Basil samples.

Keywords

Ahmad I, Beg AZ (2001). Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal Of Ethnopharmacology 74 (2):113-123. Amin GR (1991). Popular medicinal plants of Iran, vol 1. Iranian Research Institute of Medicinal Plants
Tehran.230 pp.
Aynehchi Y, Salehi Sormaghi M, Amin G, Soltani A, Qumehr N (1982). Survey of Iranian plants for saponins, alkaloids, flavonoids and tannins. II. Pharmaceutical Biology 20 (2):61-70.
Cheng G, Bai Y, Zhao Y, Tao J, Liu Y, Tu G, Ma L, Liao N, Xu X (2000). Flavonoids from Ziziphus jujuba Mill var. spinosa. Tetrahedron 56 (45):8915-8920.
Chincholkar SB, Mukerji KG (2007). Biological control of plant diseases. Crop science. Haworth Food & Agricultural Products Press, New York.426 pp.
Gülçin İ, Elmastaş M, Aboul‐Enein HY (2007). Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytotherapy
Research 21 (4):354-361.
Harman GE (2006). Overview of mechanisms and uses of Trichoderma spp. Phytopathology 96 (2):190-194. doi:10.1094/PHYTO-96-0190
Jahani M, Alemzadeh E, Motamed Rezaei O (2013). First report of Alternaria necrosis of Berberis vulgaris in Iran. International Journal of AgriScience 3 (10):743-745.
Katz GL, Shafroth PB (2003). Biology, ecology and management of Elaeagnus angustifolia L.(Russian olive) in western North America. Wetlands 23 (4):763-777.
Khan FM (2009). Ethno-veterinary medicinal usage of flora of greater cholistan desert (Pakistan). Pakistan Veterinary Journal 29 (2):75-80.
Kubicek CP, Harman GE, Ondik KL (1998). Trichoderma and Gliocladium. Taylor & Francis, London ; Bristol, PA.400 pp.
Mirzaee M, Jahani M, Mahmoudi H, Ghos K (2011). First report of jujube dieback caused by Fusarium solani. Journal of Plant Pathology 93 (4, Supplement):

Panda T, Bisaria VS, Ghose TK (1989). Method to estimate growth of Trichoderma reesei and Aspergillus wentii in mixed culture on cellulosic substrates. Applied Environmental Microbiology 55 (4):1044-1046.
Park MS, Bae KS, Yu SH (2006). Two new species of trichoderma associated with green mold of oyster mushroom cultivation in Korea. Mycobiology 34 (3):111-113. doi:10.4489/MYCO.2006.34.3.111
Petri L (1940). Review of phytopathological records noted in 1939. Boll Staz Pat veg 20 (1):1-70.
Pooyan M (1989). Medicinal plants of Southern Khorasan. Danesh Pooyesh Mashhad
Rifai MA (1969). A revision of the genus Trichoderma. Commonwealth Mycological Institute, Kew Mycological papers no 116. Commonwealth Mycological Institute, Kew, Eng.56 pp.
Russell j, Cohn R (2012). South Khorasan province. Book on Demand Ltd.86 pp.
Sajjadi SE (2006). Analysis of the essential oils of two cultivated basil (Ocimum basilicum L.) from Iran. DARU Journal of Pharmaceutical Sciences 14 (3):128-130.
Saleh AA, Esele J, Logrieco A, Ritieni A, Leslie JF (2012). Fusarium verticillioides from finger millet in Uganda. Food Additives and Contaminants: Part A 29 (11):1762-1769.
SHA Y, XING M, TANG W, WANG G, WU M, ZHU Y (2009). The diversity of pathogen strains of Jujube in the storage Acta Agriculturae Boreali-Occidentalis Sinica 2066.
Stakman EC, Fletcher DG (1930). The common Barberry and black stem rust. Farmers' Bulletin. United States Department of Agriculture, Vol 1544.29 pp.
Tehranifar A Barberry growing in Iran. XXVI
International Horticultural Congress: Asian Plants with Unique Horticultural Potential: Genetic Resources, Cultural 620, 2002. pp 193-195.