Objective: Salmonellosis is a bacterial disease with a rising prevalence in the cattle industry. It is most common in dairy calves one to ten weeks of age. Salmonellosis has a serious economic impact on the cattle industry worldwide. Methods: In May 2013, a three months old male Holstein calf with the history of lateral recumbency was referred to veterinary hospital with severe depression, cachexia, mild yellowish of sclera, bradypenea, moderate dehydration and watery feces with mucous. Blood, CSF and peritoneal fluid samples were taken and referred to laboratory. Results: In bacteriologic evaluation, serogroup D of Salmonella were isolated from all samples. Histopathologic examination showed mild fatty change with multiple typhoid nodules, hyperemia and necrosis in liver and severe hyperemia in spleen. According to clinical, post mortem examination and laboratory results, the diagnosis of chronic salmonellosis was confirmed.