Document Type: Original Article

Authors

1 Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Department of Horticultural Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Department of Horticultural Sciences, Seed and Plant Improvement Institute, Karaj, Iran

4 Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Islamic Azad University, Science and Research Branch, Tehran, Iran

Abstract

Objective: In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Methods: Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined the effect of different concentrations of iron sequestrene (Fe-EDDHA) and thiamin vitamin in ½ MS and Linsmaier and Skoog (LS) media supplemented with indol-3-butric acid (IBA) (1 mg/l) on rooting of attained plantlets. Results: According to our results, 0.4 BAP + 0.1 NAA treatment in MS medium was the best component which resulted in the highest proliferation rate (6.73). As well, we estimated the highest root number in ½ MS + 0.15 g/l Fe-EDDHA + 2.4 g/l thiamin.

Keywords

Main Subjects

Aldwinckle H and Malnoy M, 2009. Plant regeneration and transformation in the Rosaceae. Transgenic plant J., 3 (Special Issue 1):1-39.
Butiuc-Keul A, Halmagyi A, Isac V, Cr„ciunaº C and Carpa R , 2010. Apple shoot multiplication and plantlets reaction to in vitro culture. Analele Universit„˛ii din Oradea - Fascicula Biologie Tom. XVII / 1:70-75.
Castelli S, Leva AR, Eccher T and Tnxernizzi B, 1986. Comparative response of standard and spur apple cultivars to growth regulators in the in vitro culture, Acta Hortic, 179:875-876.
Chturvedi HC, Sharma PLK, Agha BQ and Sharma M, 2004. Production of cloned trees of Populus deltoides through in vitro regeneration from leaf stem and root explants and their field cultivation. Indian J. Biotechnol., 3:203-208.
Ciccotti AM, Bisognin C, Battocletti I, Salvadori A, Herdemerteus M and Jarausch W, 2008.
Micropropagation of apple proliferation resistant apomictic Malus sieboldii genotypes. Agron. Res. 6:445- 458.
Dalal MA, Das B, Sharma AK, Mir AA and Sounduri AS, 2006. In vitro cloning of apple (Malus domestica Borkh) employing forced shoot tip cultures of M9 rootstock. Indian J. Biotechnol. 5:543-553.
FAO, 2009. http://faostat.fao.org/site/567/Desktopdefault.aspx?Pag
eID=567#ancor. Hwan lee Y and Soonok K, 2005. Vitamin B1 functions as
an Activa of plant disease resistance. Plant Physiol.,138:1505-1515.
Judit Dobr·nszki, Jaime A and Teixeira da S, 2010. Micropropagation of apple ó A review. Biotechnology Advances, 28:462ñ488.
Kaushal N, Modgil M, Thakur M and Sharma DR, 2005. In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds. Indian J. Exp. Biol., 43:561-565.
Linsmaier EM, Skoog F, 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. plantarum, 18(1):100-127.
Marin JA, Jones OP and Hadlow WCC, 1993. Micropropagation of columnar apple trees. J. Hortic. Sci., 68:289ñ97.
Minaev VA, Varzilin AV and Vysotskii VA, 2003. Efficiency of clonal micropropagation and quality of improved rootstock material of apple. Sadovodstvo-iVinogradarstvo, 4:12-14.
Miri SM, Livari BV and Khalighi-Maghami SAG, 2003. Effect of carbohydrate, gibberellic acid, indole butyric acid, phloroglucinol, explant orientation and culture vessels volume on optimizing in vitro propagation of M.9
apple rootstock, Pajouhesh va Sazandegi. Agron. Hortic., 59:31-37.
Murashige T, 1974. Plant propagation through tissue culture. Annu Rev Plant Physiol, 25:135-160.
Murashige T and Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plantarum, 15 (3):473-497.
Sepahvand S, Ebadi A, Kamali K and Ghaemmaghami SA, 2012. Effects of Myo-Inositol and Thiamine on Micropropagation of GF677 (Peach × Almond Hybrid). J. Agri. Sci., 4(2):275-280.
Soni M, Thakur M and Modgil M, 2011. In vitro multiplication of Merton I. 793-An apple rootstock suitable for replantation. Indian J. Biotechnol., 10:362-
368.
Ward SP and Leyser O, 2004. Shoot branching. Curr. Opin. Plant Biol., 7:73ñ8.
Zhang Z, Wang ZZ and Zhang ZQ, 2004. Studies on regeneration system of tissue cultue in Granny Smith apple. Acta Bot. Boreali. Occidentalia Sinica, 24:621-626.
Zimmerman RH, 1984. Micropropagation of fruit plants. Acta Hortic. 120:217-222