Zohreh Bahadorloo; Zeinab Piravar; Jamshid Sabaghzadeh
Abstract
Background: Low-level laser therapy (LLLT), is a clinically well-established tool for wound healing. In vitro studies have shown that LLLT has abiostimulatory effect on mesenchymal ...
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Background: Low-level laser therapy (LLLT), is a clinically well-established tool for wound healing. In vitro studies have shown that LLLT has abiostimulatory effect on mesenchymal lineages and non-mesenchymal lineages of Adipose-derived stem cells (ADSCs) differentiation. The aim of this study was to evaluate the effects of LLLT at different energy intensities on differentiation of (ADSCs) to keratinocyte.Methods: Adipose tissue were successfully obtained from 45-year-old woman by Lipoaspirates and adipose-derived mesenchymal stem cells (ADSCs) were isolated. ADSCs were divided into a control group and three low-level laser treated groups irradiated with 0.5 J/cm2, 1 J/cm2 and 2 J/cm2 laser doses using He-Ne laser with 632nm wave length in 5 times. All groups cultured with keratinocytes media for 10-days. Cell proliferation was eveluated using 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. ADSCs developed a polygonal cobblestone shape characteristic of human keratinocytes. Specific keratinocyte marker 5 (KRT5) were analyzed by real-time PCR. Data were analyzed using one-way AVOVA and Tukey-Kramer hoc test by SPSS software version 16.Results: Results of this study demonstrated LLLT could significantly affect proliferation of ADSCs (P<0.05) and promote ADSCs differentiation to keratinocytes by increasing expression of KRT5 at of 1 J/cm2 and 2 J/cm2 laser doses (P<0.01). LLLT at density of 0.5 J/cm2 could not promote ADSCs differentiation significantly.Conclusion: Considering these findings, LLLT could improve current in vitro methods of differentiating ADSCs to keratinocyte prior to transplantation. Thereby LLLT can use clinically as promoting skin wound healing.
