%0 Journal Article %T Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan %J International Journal of Advanced Biological and Biomedical Research %I Sami Publishing Company %Z 2383-2762 %A Amin, Shahid %A ur Rahman, Sajjad %A Hussain, Iftikhar %A Muhammad, Ghulam %A Arif Awan, Mohammad %A Ahmad, Zafer %A Masood Tariq, Mohammad %A Irfan Ullah, Muhammad %A Azam Kakar, Muhammad %D 2016 %\ 03/01/2016 %V 4 %N 1 %P 32-39 %! Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan %K M.ovinpeumoniae %K Sheep %K Biochemical %K PCR %K Balochistan %R 10.26655/ijabbr.2016.2.4 %X Mycoplasma ovipneumoniae is an important respiratory pathogen of sheep across the world. In the present study nasal swab samples (n=778) were processed and 51isolates of M. ovipneumoniae were identified through biochemical tests and PCR. Difference in the isolation rate among districts (n=12), age groups (n=3), breads (n=6) was assessed. Similarly the isolate rate among healthy (n=513) and respiratory distressed (n=265) sheep were also compared. The results for the district-wise isolation of Mycoplasma ovipneumoniae in sheep showed non-significant variations among districts of Balochistan (2= 1.65, P=0.9990). Statistically significant difference (2 = 7.698, P< 0.02) in the isolation of Mycoplasma ovipneumoniae among various age groups was recorded, while highest isolation was seen in sheep of < 1 year.Statistically highly significant difference (2 = 16.6, P <0.0050) in the isolation of Mycoplasma ovipneumoniae among various breeds of sheep in Balochistan was seen, whereas highest isolation rate (3.70%) was observed in Harnai breed for sheep Furthermore, statistically highly significant difference (Fisher's exact test P≤0.0001) in the isolation of Mycoplasma ovipneumoniae between healthy and respiratory distressed sheep breeds in Balochistan was found. %U https://www.ijabbr.com/article_19136_0f28e4af9267691af42f9302b11d73a5.pdf