TY - JOUR ID - 12214 TI - Study of Factors Influencing Somatic Embryogenesis in Rice (Oryza Sativa L.) JO - International Journal of Advanced Biological and Biomedical Research JA - IJABBR LA - en SN - 2383-2762 AU - Ghobeishavi, Hamideh AU - Dorani Uliaie, Ebrahim AU - Alavikia, Seyed Siamak AU - Valizadeh, Mostafa AD - Graduated of Master of Science in Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz, Iran AD - Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of agriculture, University of Tabriz, Tabriz, Iran Y1 - 2015 PY - 2015 VL - 3 IS - 1 SP - 43 EP - 50 KW - Rice KW - Mature embryo KW - somatic embryogenesis KW - PEG KW - AgNO3 KW - Activated charcoal DO - N2 - Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Skoog 1962) supplemented with 30 g/L sucrose, 7g/L agar and 2 mg/L 2,4-D. Results: Significant difference was observed between two variety in callus induction. Somatic embryogenesis and the plant regeneration were influenced by the genotype as well as composition of the medium used. The callus induction rate and callus morphology were different depend on genotypes. High somatic embryogenesis was obtained in medium (MS medium containing 2 mg/L 2,4-D) supplemented with 3 mg/L AgNO3, 9 gr/L agar and 20 gr/L PEG, respectively. The high frequency plant regeneration was achieved from both varieties in medium supplemented with 9 and 11gr/L agar and 5 mg/L AgNO3. UR - https://www.ijabbr.com/article_12214.html L1 - https://www.ijabbr.com/article_12214_d2222de987f25b788db11c1d2bf7e6fd.pdf ER -