The aim of this study was to determine influencing factors on participation of fishermen member cooperative in sustainable fishing in Golestan Province. The methodological approach of this study was descriptive- correlative. The research population consisted of 144 fishermen who member cooperative, which were selected using census method. Validity of the instrument was established by a panel of experts consisting of senior faculty members in agricultural extension and education department, and research committee advisors. Reliability analysis was conducted by using and Cronbach alpha formula and result was 88. The results showed that 11.4, 71.2 and 17.4 percent of fishermen expressed their participation in sustainable fishing were moderate, good and very good respectively. Also variables of effect of extension- education activities, satisfaction of cooperative, social participation and attitude toward sustainable fishing with participation in sustainable fishing had been relationship of positive and significant. The results of the multiple regression analysis (stepwise method) revealed that the variable of effect of extension- education activities explained a variation of 31.7% of the participation in sustainable fishing.