Document Type : Original Article


Department of Agriculture, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran


Objective: In order to investigate effects of different concentrations of poly amines Sprimidin and Putrescine on germination of Arg cultivar under salinity stress. Methods: This experiment was conducted as factorial based on completely randomized design with four replicates in agronomy and plant breeding laboratory, Agricultural department, Islamic Azad University, Varamin-Pishva Branch in spring of 2014. First factor was four levels of salinity including S1: 3.87, S2: 5.95, S3: 10.25 and S4: 12.87 ds/m and the second factor was three levels of poly amines including H0: control (not application of poly amines), H1: Putrescine 2.5 m/mol and H2: Sprimidin 5 mmol/L. coleoptile and cleorhiza length, cleorhiza number, coleoptile, cleorhiza and seedling dry weight, Tissue water content (TWC), germination percent and number of damaged seeds were measured. Results: Analysis of variance showed that coleoptile and cleorhiza length, cleorhiza dry weight affected by salinity and hormone levels at 1% of probability level. There was no significant difference among other treatments for other properties. Results of this experiment indicated that following increasing salinity, sever reduction in germination-dependent properties were observed.


Basalah MO, Mohammad S 1999. Effect of salinity and plant growth regulators on seed germination of Medicagosativa L. Pakistan J Biol Sci 2: 651-653
Gurmani, A.R., Bano, A., Din, J., Khan, S.U., Hussain, I., 2009. Effect of phytohormones on growth and ion accumulation of wheat under salinity stress. Afr. J. Biotechnol.8, 1887ñ1894.
Hisamatsu T, Koshioka M, Kubota S, Fujime Y, King RW, Mander LN 2000. The role of gibberellin in the control of growth and flowering in Matthiola incana. Physiol Plantarum 109: 97-105
Iqbal, M., Ashraf, M. 2010, Changes in hormonal balance: a possible mechanism of pre- sowing chillinginduced salt tolerance in spring wheat. J. Agron. Crop Sci. doi:10.1111/j.1439-37X.2010.00434.x.
Iqbal, M., Ashraf, M., 2005a. Changes in growth, photosynthetic capacity and ionic relations in spring wheat (Triticum aestivum L.) due to pre-sowing seed
treat- ment with polyamines. Plant Growth Regul. 46, 19ñ30
Khan MA, Gul B, Weber DJ 2004. Action of plant growth regulators and salinity on the seed germination of Ceratoides lanata. Can. J. Bot. 82:37-42.
Kiseleva, I.S., Kaminskaya, O.A., 2002. Hormonal regulation of assimilate utilization in barley leaves in relation to the development of their source function. Russ. J. Plant Physiol. 49, 534ñ540.
Lehmann J, Atzorn R, Bruckner C, Reinbothe S, Leopold J, Wasternack C, Parthier B 2000. Accumulation of jasmonate, abscisic acid, specific transcripts and proteins inosmotically stressed barley leaf segments. Planta 197:156-162
Mo, H., Pua, E.C., 2002. Up-regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea) in response to stress. Physiol. Plant 114, 439ñ449.
Olszewski N, Sun TP, Gubler F 2002. Gibberellin signaling,biosynthesis, catabolism, and response pathways. Plant Cell 14: 561-580
Tun, N.N.; Santa-Catarina, C.; Begum, T.; Silveira, V.; Handro, W.; Floh, E.I.S.;. 2006, Scherer, G.F.E.Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings.Plant Cell Physiol 47, 346ñ
Urano, K.; Hobo, T.; Shinozaki, K. 2005, Arabidopsis ADC genes involved in polyamine biosynthesis are essential for seed development. FEBS Lett. 579, 1557ñ1564.
Yamaguchi S, Kamiya Y 2000. Gibberellin biosynthesis, its regulation by endogenous and environmental signals. Plant Cell Physiol 41: 251-257
Yoda, H.; Fujimura, K.; Takahashi, H.; 2009, Munemura, I.; Uchimiya, H.; Sano, H. Polyamines as a common source of hydrogen peroxide in host- and nonhost hypersensitive response during pathogen infection. Plant Mol. Biol. 70, 103ñ112.
Zhu, H., Ding, G. H., Fang, K., Zhao, F. G. and Qin, P. 2006. New perspective on the mechanism of alleviating salt stress by spermidine in barley seedlings. Plant Growth Regulation 49: 147-156