Document Type : Original Article


Varamin, Pishva Branch, Islamic Azad University, Varamin, Iran


Objective: In order to investigate effects of different concentration of gibberlic acid (GA3), kentin and Putrescinepoly amine, on germination of three cultivars of wheat under salinity stress. Methods: The experiment was carried out as factorial based on completely randomized design with four replicates in laboratory of Agronomy and Plant Breeding, department of agriculture in Islamic Azad University, Varamin-Pishva branch in 2014. The first factor was four levels of salinity including S1: 3.78, S2: 5.95, S3: 10.25 and S4: 12.87 ds/m, the second factor was three cultivar of spring wheat including V1: Tajan, V2: Arg and V3: Pishtaz. The third factor was six level of hormone including H0: control, H1: GA3 150 mg/L, H2: Kentin 150 mg/L, H3: GA3 150 mg/L + Kentin 150 mg/L, H4: Putrescine 2.5 mmol/L, H5: GA3 150 mg/L + Kentin 150 mg/L + Putrescine 2.5 mmol/L. Coleoptile and Cleorhiza length, Cleorhiza number, Coleoptile, Cleorhiza and Seedling Dry Weight, Tissue Water Content (TWC), Germination Percent and Number of Damaged Seeds were measured. Results: Analysis of variance showed that Coleoptile and cleorhiza length, cleorhiza number, seedling dry weight and TWC affected by salinity, hormone and Cultivar at 1% of probability level, but not significant in other properties. In general, results of this experiment showed that application of different treatments of hormone and poly amine had no significant effect on reduction of salinity effect in germination process.


Abdullah Z, Ahmad R ,1990. Effect of pre- and postkinetin treatments on salt tolerance of different potato cultivars growing on saline soils. J Agron Crop Sci 165: 94-102
Afzal, I., S.A.M. Basra, A. Hamid and M. Farooq. 2006. Phisiological enhancements for alleviation of salt stress in wheat. Pak. J. Bot. 38: 1649-1659.
Afzal, I., S. Basra, and A. Iqbal, 2005. The Effect of Seed Soaking With Plant Growth Regulators on Seedling Vigor of Wheat Under Salinity Stress. J. Stress Physiol Biochem. 1: 6-14
Akman Z. 2009. Effects of GA3 and kinetin pre-sowing treatments on seedling emergence and seedling growth in wheat under saline conditions. J. Anim. Vet. Adv. 8:362-367.
Argueso CT, Ferreira FJ, Kieber JJ, 2009. Environmental perception avenues: the interaction of cytokinin and environmental response pathways. Plant Cell Environ 32:1147-1160
Alonso-Ramirez, A., RodrÌguez, D., Reyes, D., Jimenez, J.A., Nicolas, G., Lopez-Climent, M., Gomez-Cadenas, A., Nicolas, C., 2009. Evidence for a role of gibberellins in salicylic acid- modulated early plant responses to abiotic
stress in Arabidopsis seeds. Plant Physiol. 150, 1335ñ 1344.
Ashraf, M. 2004. Some important physiological selection criteria for salt tolerance in plants. Flora. 199: 361-376.
Angelov, G. B. 2003. Isoenzyme variation of esterase and acid phosphatase and genetic affinities among Dasypyrum villosum. Turkish Journal of Botany, 27, 249- 254.
Barret-Lenard, E. G., Robson, A. D. and Greenway, H. 1999. Effect of phosphorus deficiency and water definition on phosphatase activity from wheat leaves. Journal of Experimental Botany, 33, 682-693.
Blumwald, E. 2000. Sodium transport and salt tolerance in plants.Current Opinion in Cell Biology. 12:431-434.
Boucaud J, and Ungar IA, 1976. Hormonal control of germination under saline conditions of three halophyte taxa in genus Suaeda. Physiol Plant 36: 197-200
Chakrabarti N, Mukherji S 2003, Alleviation of NaCl stress by pretreatment with phytohormones in Vigna radiata. Biol Plantarum 46: 589-594.
Chartzoulakis, K and G. Klapaki. 2000. Response of tow greenhouse pepper hybrids to NaCl salinity during different growth stages. Sci. Hortic. 86: 247-260.
Cleland, R. E. 1987. Auxin and cell elongation. In: P.J. Davies (ed). Plant hormones and their role in plant growth and development. Kluwer. Dordrecht, The Netherland. pp: 132-148.
Davies P J , 2004. Plant hormones: biosynthesis, signal transduction, action. Kluwer Academic Press, the Netherlands.
Dewey, D. R, 1962. Breeding crested wheat grass for salt tolerance. Crop Sci.2: 403-407.
Francodantas,B., L. De Saribeiiro and C. Albertoaragao, 2005. Phisiological response of cowpea seeds to salinity stress. Rev. Bras. Sementes. 27: 144-148
Grattan, S. R. and Grieve, C. M. 2000. Salinity- mineral nutrient relations in horticultural crops. ScientiaHorticulturae, 78, 127-157.
Gul B, and Khan MA ,2008. Effect of compatible osmotica and plant growth regulators in alleviating salinity stress on the seed germination of Allenrolfea occidentalis. Pak. J. Bot. 40:1957-1964.
Hare, P. D., W.A. Cress and J. van Staden. 1997. The involvement of cytokinin in plant responses to environmental stress. Plant Growth Regulation. 23: 79ñ
Iqbal M, Ashraf M, Jamil A, 2006a. Seed enhancement with cytokinins: changes in growth and grain yield in saltstressed wheat plants. Plant Growth Regul 50: 29-39
Iqbal M, Ashraf M, Jamil A, Ur-Rehman S ,2006b. Does seed priming induce changes in the levels of some endogenous plant hormones in hexaploid wheat
plantsunder salt stress? J Integ Plant Biol 48:81-189  Iqbal, M., and Ashraf, M., 2005a. Changes in growth, photosynthetic capacity and ionic relations in spring wheat (Triticum aestivum L.) due to pre-sowing seed treat- ment with polyamines. Plant Growth Regul. 46, 19ñ30.
Iqbal, M., and Ashraf, M., 2005b. Presowing seed treatment with cytokinins and its effect on growth, photosynthetic rate, ionic levels and yield of two wheat cultivars differing in salt tolerance. J. Integr. Plant Biol. 47, 1315ñ1325.
Ismail AM 2003. Physiological studies on the influence of cytokinin or GA3 in the alleviation of salt stress in sorghum plants. Acta Agro. Hung. 51:371-380.
Kirkham MB, Gardner WR, Gerloff GC 1974. Internalwater status of kinetin-treated, salt-stressed plants. PlantPhysiol 53: 241-243 Letham DS 1978.
Cytokinins. In: Letham DS, Goodwin PB, HigginsTJV(ed) Phytohormones and related compounds.Vol 1. Elsevier, Amsterdam, pp. 205-243
Merchan F, de Lorenzo L, Rizzo SG, Niebel A, Manyani H,Frugier F, Sousa C, Crespi M 2007. Identification of regulatory pathways involved in the reacquisition of root growth after salt stress in Medicago truncatula. Plant J 51:1-17.
Mizrahi, Y., A. Blumonfeld., S. Bittner and A. E. Richmond. 1971. Abscisic acid and cytokinin content of leaves in relation to salinity and relative humidity. Plant Physiology. 48:752-755.
Sarapatka, B.,Dudova, L. and Kroskova, M. 2004. Effect of pH and phosphate supply on acid phosphatase activity in cereal roots. Biologia Bratislova, 59, 127-131.
Tsonev, T.D., G. N. Lazova., Z. G. Stoinova and L. P. Popova. 2000. A possible role for Jasmonic acid in adaptation of barley seedlings to salinity stress. Plant Growth regulation. 17:153-159.