Document Type : Original Article


1 Graduate student, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Graduate student, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran


Objective: In recent years, antiviral drugs have been used widely in the glob to prevent and treat viral diseases. They injected into eggs to eliminate pathogens and prevent of egg transmission of disease, but the adverse effects of drugs have always been a major concern. There is little information available about the safety of antiviral drugs in the embryonated eggs of birds. The objective of this study was to evaluation of the effect of acyclovir on the viability of chicken embryo during the first trimester of the incubation period. Methods: Fertile chicken eggs were divided into two equal treatment groups. The phosphate buffered saline injected group and acyclovir-injected group whose individuals were injected with acyclovir solution at a dosage of 80 mg per Kg egg-weight. Embryos monitored daily during incubation until day 10 after which; they were examined for viability. Results: Results showed that all of the embryos were dead in the acyclovir-injected group. Based on findings, it is concluded that acyclovir at above-mentioned concentration has a severe lethal effect on the chicken embryo. So, acyclovir egg-injection cannot be used toeliminate pathogens and prevention of egg transmission of the disease


Main Subjects

Agunos A, LÈger D, Carson C (2012). Review of antimicrobial therapy of selected bacterial diseases in broiler chickens in Canada. Canadian Vet. J. 53(12): 1289.
Ahrens FA, Martin RJ (2013). Antimicrobial drugs. Handbook of Veterinary Pharmacology 347.
Altunsoy A, Aypak C, Azap A, Ergin¸l , Balik (2011). The impact of a nationwide antibiotic restriction program on antibiotic usage and resistance against nosocomial pathogens in Turkey. Int. J. Med. Sci. 8(4):
Banerjee S, Mukhopadhayay SK, Ganguly S (2013). Phytogenic Growth Promoter as Replacers for Antibiotic Growth Promoter in Poultry Birds. J. Anim. Genet. Res. 1(1): 6-7.
Buscaglia C (2013). Influence of the addition of antibiotics on survival of herpevirus of turkeys. Avian Dis. 52(2): 437-436.
Colomer-Lluch M, Imamovic L, Jofre J, Muniesa M (2011). Bacteriophages carrying antibiotic resistance genes in fecal waste from cattle, pigs, and poultry. Antimicrob. Agents Chemother. 55(10): 4908-4911.
Conway GR, Pretty JN (2013). Unwelcome harvest: agriculture and pollution, Routledge.
Crespo R, Shivaprasad H, Silva Franca M (2013). Ulcerative Enteritis-like Disease Associated with Clostridium sordellii in Quail. Avian Dis. 57(3): 213-221.
Erdem H, Akova M (2012). Leading infectious diseases problems in Turkey. Clin. Microbiol. Infect. 18(11): 1056- 1067.
Ghazikhanian GY, Yamamoto R, McCapes R, Dungan WM, Larsen C, Ortmayer H (1980). Antibiotic Egg Injection to Eliminate Disease II. Elimination of Mycoplasma meleagridis from a Strain of Turkeys. Avian Dis. 48-56.
Hamburger V (1942). A manual of experimental embryology, University of Chicago Press Chicago.
Hasan B, Faruque R, Drobni M, Waldenstrˆm J, Sadique A, Ahmed KU, Islam Z, Parvez MH, Olsen B, Alam M (2011). High prevalence of antibiotic resistance in pathogenic Escherichia coli from large-and small-scale poultry farms in Bangladesh. Avian Dis. 55(4): 689-692.
Jones K, Thornton J, Zhang Y, Mauel M (2013). A 5-year retrospective report of Gallibacterium anatis and Pasteurella multocida isolates from chickens in
Mississippi. Poult. Sci. 92(12): 3166-3171.
Kabir S (2010). Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns. Int. J. Environ. Res. Public. Health 7(1): 89-114.
Kleven S (2008). Control of avian mycoplasma infections in commercial poultry. Avian Dis. 52(3): 367-374.
Lee K-W, Ho Hong Y, Lee S-H, Jang SI, Park M-S, Bautista DA, Donald Ritter G, Jeong W, Jeoung H-Y, An D-J (2012). Effects of anticoccidial and antibiotic growth promoter programs on broiler performance and immune status. Res. Vet. Sci. 93(2): 721-728.
Lee S-K, Chon J-W, Song K-Y, Hyeon J-Y, Moon J-S, Seo K-H (2013). Prevalence, characterization, and antimicrobial susceptibility of Salmonella Gallinarum isolated from eggs produced in conventional or organic farms in South Korea. Poult. Sci. 92(10): 2789-2797.
MacDonald JM, Wang S-L (2011). Foregoing subtherapeutic antibiotics: The impact on broiler grow-out operations. App. Economic Perspectives Policy 33(1): 79-98.
McDougald L, Abraham M, Beckstead R (2012). An Outbreak of Blackhead Disease (Histomonas meleagridis) in Farm-Reared Bobwhite Quail (Colinus virginianus). Avian Dis. 56(4): 754-756.
Nemati M (2013). Antimicrobial resistance of porteus isolates from poultry. Europ. J. Experiment. Biol. 3(6):499-500.
Obeng AS, Rickard H, Ndi O, Sexton M, Barton M (2012). Antibiotic resistance, phylogenetic grouping and virulence potential of Escherichia coli isolated from the faeces of intensively farmed and free range poultry. Vet. Microbiol. 154(3): 305-315.
Priyantha M, Vipulasiri A, Gunawardana G (2012). Salmonella control in poultry breeder farms in Sri Lanka: Effects of oral antibiotic treatment on whole blood agglutination test with Salmonella pullorum antigen. Int. J. Livestock Product. 3(2): 21-24.
Richardson HW (1997). Handbook of copper compounds and applications, CRC Press.
Rigobelo EC, Blackall PJ, Maluta RP, £vila FAd (2013). Identification and antimicrobial susceptibility patterns of Pasteurella multocida isolated from chickens and japanese quails in Brazil. Braz. J. Microbiol. 44(1): 161-164.
Sapkota AR, Hulet RM, Zhang G, McDermott P, Kinney EL, Schwab KJ, Joseph SW (2011). Lower prevalence of antibiotic-resistant enterococci on US conventional poultry farms that transitioned to organic practices. Environ. Health Perspect. 119(11): 1622.
Sheeks OB, Sheeks RL (1992). Egg injection method, apparatus and carrier solution for improving hatchability and disease control, Google Patents.
Singroha R, Srivastava S, Chhikara P (2012). Effect of Gentamicin on kidney in developing chicks. Eur J Anat 16(2): 119-126.
Singroha R, Srivastava S, Chhikara P (2013). Effect of gentamicin on proximal convoluted tubules of kidney in developing chicks. J. Anat. Soc. India 62(1): 17-22.
Swayne D, Glisson JR, McDougald L, Nolan LK, Suarez DL, Nair VL (2013). Diseases of Poultry, Wiley-Blackwell. Sweetman SC, Pharm B, PharmS F, Eds. (2009). Martindale: The Complete Drug Reference. London, Pharmaceutical Press.
Tavakkoli H, Derakhshanfar A, Noori Gooshki S (2013). A short preliminary experimental study on teratogenic effect of methenamine in embryonic model. International Journal of Advanced Biological and Biomedical Research.
1(12): 1523-1528.
Tavakkoli H, Derakhshanfar A, Noori Gooshki S (2014a). The effect of florfenicol egg-injection on embryonated chicken egg. International Journal of Advanced Biological and Biomedical Research. 2(2): 496-503.
Tavakkoli H, Derakhshanfar A, Noori Gooshki S (2014b). Toxicopathological lesions of fosfomycin in embryonic model. Euro. J. Experiment. Biol. 4(2): 63-71.
Tavakkoli H, Derakhshanfar A, S S (2014c). Toxicology of urotropine in chicken embryo model. Online J Vet Res 18(2): 109-115.
Tavakkoli H, Derakhshanfar A, Salandari S (2104). ovo administration in chicken embryo. 
Wightwick AM, Salzman SA, Reichman SM, Allinson G, Menzies NW (2013). Effects of copper fungicide residues on the microbial function of vineyard soils. Environment. Sci. Pollution Res. 20(3): 1574-1585.