Document Type : Original Article

Authors

1 Department Infectious Disease and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran

2 Zabol Medicinal plant Research Center, Zabol, Iran

3 Zabol University of Medical Sciences, Zabol, Iran

4 Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Kerman, IR Iran

5 Biochemistry Department., Payame Noor University, Mashhad, I.R. of Iran

10.26655/ijabbr.2016.2.15

Abstract

The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. The aim of this study was to determined antibacterial activity of silver nanoparticles produced by Rosmarinus officinalis L leaf extract against some human pathogenic bacteria. The formation and characterisation of AgNPs were confirmed by UV-Vis spectroscopy, energy-dispersive spectroscopy (EDX), X-ray diraction (XRD) and transmission electron microscope (TEM). All strains were obtained from standard laboratory and the minimum inhibitory concentrations were investigated by microdulition method.The result show that, the levels of MIC was observed ranges from 1.25 to 2.5 mg/ml. The highest MIC value was observed against S.pneumoniae, Hafnia alvei, S. saprophyticus.

Keywords

Ahearn, D.G., May, L.L., Gabriel, M.M., 1995. Adherence of organisms to silver-coated surfaces. J. Ind. Microbiol., 15, 372–376.
Cho, K.H., Park, J.E., Osaka, T., Park, S.G., 2005. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim. Acta., 51, 956–960.
Chou, W.L., Yu, D.G., Yang, M.C., 2005. The preparation and characterization of silver-loading cellulose acetate hollow fiber membrane for water treatment. Polym. Adv. Technol., 16, 600–607.
Davis, H.R., 1997. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing (2002), 16th International supplement. CLSI document M100-S12.
G Guzman, M., Dille, J., Godet, S., 2009. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int. J. Chem. Biomol. Eng., 2, 3.
Ghandour, W., Hubbard, J.A., Deistung, J., Hughes, M.N., Poole, R.K., 1988. The uptake of silver ions by Escherichia coli K12: toxic effects and interaction with copper ion. Appl. Microbiol. Biotechnol., 28, 559–565.
Jeong, S.H., Yeo, S.Y., Yi, S.C., 2005. The effect of filler particle size on the antibacterial properties of compounded polymer/silver fibers. J. Mater. Sci., 40, 5407–5411.
Lo, A.H., Liang, Y.C., Lin-Shiau, S.Y., Ho, C.T., Lin, J.K., 2002. Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down–regulating nuclear factor–κB in mouse macrophages.
Carcinogenesis. 23, 983-991.
Ouattara, B., Simard, R.E., Holley, R.A., Piette, G.J.-P., Begin, A., 1997. Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms. Int. J. Food. Microbiol., 37, 155-162.
Parikh, D.V., Fink, T., Rajasekharan, K., Sachinvala, N.D., Sawhney, A.P.S., Calamari, T.A., Parikh, A.D., 2005.
Antimicrobial silver/sodium carboxymethyl cotton dressings for burn wounds. Text. Res. J., 75, 134–138.
Rupp, M.E., Fitzgerald, T., Marion, N., Helget, V., Puumala, S., Anderson, J.R., Fey, P.D., 2004. Effect of silvercoated urinary catheters: Efficacy, cost-effectiveness, and antimicrobial resistance. Am. J. Infect. Control., 32, 445–450.
Samuel, U., Guggenbichler, J.P., 2004. Prevention of catheter-related infections: The potential of a new nano-silver impregnated catheter. Int. J. Antimicrob. Agent., 23, 75–78.
Schreurs, W.J.A., Rosenberg, H., 1982. Effect of silver ions on transport and retention of phosphate by Escherichia coli. J. Bacteriol., 152, 7–13.
Sondi, I., Salopek-Sondi, B., 2004. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J. Colloid. Interf. Sci., 275, 177.
Sondi, I., Salopek-Sondi, B., 2004. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteri. J. Colloid. Interf. Sci., 275, 177–182.
Spadaro, J.A., Berger, T.J., Barranco, S.D., Chapin, S.E., Becker, R.O., 1974. Microb. Agent. Chemother., 6, 637.
Ulkur, E., Oncul, O., Karagoz, H., Yeniz, E., Celikoz, B., 2005. Comparison of silvercoated dressing (Acticoat™), chlorhexidine acetate 0.5% (BactigrassW), and fusidic acid 2% (FucidinW) for topical antibacterial effect in
methicillin-resistant staphylococci-contaminated, full-skin thickness rat burn wounds. Burns, 31, 874–877.
Vaidyanathan, R., Kalishwaralal, K., Gopalram, S., Gurunathan, S., 2009. Nanosilver: the burgeoning ther-apeutic molecule and its green synthesis. Biotechnol. Adv., 27, 924-937.
Yuranova, T., Rincon, A.G., Bozzi, A., Parra, S., Pulgarin, C., Albers, P., Kiwi, J., 2003. Antibacterial textiles prepared by RF-plasma and vacuum-UV mediated deposition of silver. Photochem. Photobiol. A., 161, 27–34.