Document Type : Original Article


1 Department of Food Science and Technology, Zahedan University of Medical Sciences, Zahedan, Iran

2 Department of biochemistry, school of medicine, Zabol University of medical sciences, Zabol, Iran

3 Emergency medicine specialist, Zabol University of medical sciences, Zabol, Iran

4 Department of Anatomical sciences, Faculty of medicine, Zabol University of medical sciences, Zabol, Iran

5 Department of Neurosurgery, Amiralmomenin Hospital, Zabol University of medical sciences, Zabol, Iran

6 Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran


Introduction: Bacterial resistance to antibiotics, the first major concern in the 1960s, has reappeared worldwide over the past 20 years. Because these bacteria are not resistant to various conventional therapies, the medicinal and herbal plants used in different countries should be evaluated for their therapeutic potential. These valuable biological resources are a repository of complex active molecules. Therefore, in this study, we tried to evaluate the antimicrobial activity of some medicinal plant extracts on Staphylococcus aureus isolated from humans.
Methods: Rhazya stricta was collected and their species were identified in the botanical laboratory of University of Zabol. To prepare plant extract, 40 g of dried leaves were used in 400 cc of solvent (aqueous, ethanol, methanol, ethyl acetate, and hydro-alcoholic). The various strains of Staphylococcus aureus used in this study were isolated from the human nose and identified by biochemical, bacteriological and growth tests as well as standard tests. Antimicrobial effects were investigated by well diffusion method in Müller Hinton agar medium. Statistix ver10 software was used for statistical calculations. Mean comparison was performed using the LSD at the level of one percent and Excel software was also used to draw the shapes.
Results: Rhazya stricta extracts had different inhibitory zone diameters against Staphylococcus aureus at 100 ppm dilution (p <0.01). R. stricta ethanolic extract had the greatest effect (average 8.3 mm) on inhibiting the growth of 6 strains of S. aureus. Then aqueous and hydroalcoholic extracts with an average of 7 mm were in the next ranks. The ethyl acetate extract had the lowest effect on the inhibition of S. aureus. The lowest MIC of R. stricta ethanolic extract against S. aureus samples was 3.1 ppm, which was inhibited by four strains. The lowest MBC was 6.2 ppm.
Conclusion: Considering the side effects of chemical drugs and antibiotics as well as the potential effect of ethanolic extract of Rhazya stricta on Staphylococcus aureus, it is recommended to use ethanol solvent to evaluate the antimicrobial activity of R. stricta.

Graphical Abstract

Evaluation of Antimicrobial Activity of Rhazya Stricta (Apocynaceae) Extract Prepared with Different Solvents on Staphylococcus Aureus (Staphylococcaceae) Isolated from Humans


Main Subjects

1. Khan R, Baeshen M N, Saini K S, Bora R S, Al-Hejin A M, Baeshen N A. (2016). Antibacterial activities of Rhazya stricta leaf extracts against multidrug-resistant human pathogens. Biotechnology & Biotechnological Equipment, 30(5): 1016-1025.
2. Fazeli-Nasab B, Sayyed R Z, Sobhanizadeh A. (2021). In Silico Molecular Docking Analysis of α-Pinene: An Antioxidant and Anticancer Drug Obtained from Myrtus communis. International Journal of Cancer Management, 14(2): e89116.
3. Fazeli-Nasab B, Khajeh H, Rahmani A F. (2021). Effects of culture medium and plant hormones in organogenesis in olive (CV. Kroneiki). J. Plant Bioinform. Biotech., 1(1): 1-13.
4. Fazeli-Nasab B. (2021). Biological Evaluation of Coronaviruses and the Study of Molecular Docking, Linalool, and Thymol as orf1ab Protein Inhibitors and the Role of SARS-CoV-2 Virus in Bioterrorism. [Research]. journal of ilam university of medical sciences, 28(6): 77-96. https:///
5. Davari A, Solouki M, Fazeli-Nasab B. (2018). Effects of jasmonic acid and titanium dioxide nanoparticles on process of changes of phytochemical and antioxidant in genotypes of Satureja hortensis L. Eco-Phytochemical Journal of Medicinal Plants, 5(4): 1-20.
6. Rasouli H, Farzaei M H, Khodarahmi R. (2017). Polyphenols and their benefits: A review. International Journal of Food Properties, 20(sup2): 1700-1741.
7. Mehrabi A-A, Fazeli-Nasab B. (2012). In vitro culture of Allium scorodoprasum spp. Rotundum: callus induction, somatic embryogenesis and direct bulblet formation. Intl. J. Agri. Crop Sci, 4(1): 1-7.
8. Fazeli-nasab B, Fooladvand Z. (2014). Classification and Evaluation of medicinal plant and medicinal properties of mastic. International Journal of Advanced Biological and Biomedical Research, 2(6): 2155-2161.
9. Yang Y, Xi-Qiang L, Chun-Ping T. (2008). Natural products chemistry research 2006's progress in China. Chin J Nat Med, 6(1): 70-78.
10. Islam S, Rahman A, Sheikh M I, Rahman M, Jamal A H M, Alam F. (2010). In vitro antibacterial activity of methanol seed extract of elettaria cardamomum (L.) maton. Agriculturae Conspectus Scientificus, 75(3): 113-117.
11. Rasouli H, Yarani R, Pociot F, Popović-Djordjević J. (2020). Anti-diabetic potential of plant alkaloids: Revisiting current findings and future perspectives. Pharmacological research, 155: 104723.
12. Fooladvand Z, Fazeli-nasab B. (2014). Antibacterial activities of Stachys lavandulifolia Vahl. extract against eight bacteria. Journal of Herbal Drugs (An International Journal on Medicinal Herbs), 5(1): 13-18.
13. Saeidi S, Fazeli-Nasab B. (2019). Evaluation of antibacterial and antifungal activity of various extracts of the Rhazya stricta, Capparis spinosa, cretica Cressa. New Findings in Veterinary Microbiology, 2(1): 57-66.
14. Shehzad A, Qureshi M, Jabeen S, Ahmad R, Alabdalall A H, Aljafary M A, Al-Suhaimi E. (2018). Synthesis, characterization and antibacterial activity of silver nanoparticles using Rhazya stricta. PeerJ, 6: e6086. PubMed: 30588401 ;
15. Iqbal S, Bhanger M, Akhtar M, Anwar F, Ahmed K R, Anwer T. (2006). Antioxidant properties of methanolic extracts from leaves of Rhazya stricta. Journal of medicinal food, 9(2): 270-275.
16. Al-Dabbagh B, Elhaty I A, Al Sakkaf R, El-Awady R, Ashraf S S, Amin A. (2018). Antioxidant and anticancer activities of Trigonella foenum-graecum, Cassia acutifolia and Rhazya stricta. BMC Complementary and Alternative Medicine, 18(1): 1-12.
17. Marwat S K, Usman K, Shah S S, Anwar N, Ullah I. (2012). A review of phytochemistry, bioactivities and ethno medicinal uses of Rhazya stricta Decsne (Apocynaceae). African Journal of Microbiology Research, 6(8): 1629-1641.
18. Abadi F, Abdulaziz A, Hadhoud A, Baeshin N, Qari S, Alhejin A. (2011). An epidemiological survey and evaluation of the antimicrobial growth effect of Rhazya stricta (Decne) leaves extract on different genotypes of Neisseria meningitides. Egypt J Med Microbiol, 20(2): 77À86.
19. Fazeli-nasab B, Moshtaghi N, Forouzandeh M. (2019). Effect of Solvent Extraction on Phenol, Flavonoids and Antioxidant Activity of some Iranian Native Herbs. Scientific Journal of Ilam University of Medical Sciences, 27(3): 14-26
20. Bautista-Trujillo G, Solorio-Rivera J, Renteria-Solorzano I, Carranza-German S, Bustos-Martinez J, Arteaga-Garibay R, Baizabal-Aguirre V, Cajero-Juarez M, Bravo-Patino A, Valdez-Alarcon J. (2013). Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis. Journal of medical microbiology, 62(3): 369-376.
21. Talib W H, Mahasneh A M. (2010). Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules, 15(3): 1811-1824.
22. Owuama C I. (2017). Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African Journal of Microbiology Research, 11(23): 977-980.
23. Lambert R, Pearson J. (2000). Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values. J Appl Microbiol, 88(5): 784-790.
24. Pervin M, Hasnat M A, Lim B O. (2013). Antibacterial and antioxidant activities of Vaccinium corymbosum L. leaf extract. Asian Pacific Journal of Tropical Disease, 3(6): 444-453.
25. Česonienė L, Jasutienė I, Šarkinas A. (2009). Phenolics and anthocyanins in berries of European cranberry and their antimicrobial activity. Medicina, 45(12): 992-999. PMID: 20173403
26. Shen X, Sun X, Xie Q, Liu H, Zhao Y, Pan Y, Hwang C-A, Wu V C. (2014). Antimicrobial effect of blueberry (Vaccinium corymbosum L.) extracts against the growth of Listeria monocytogenes and Salmonella Enteritidis. Food control, 35(1): 159-165.
27. Rosaline X D, Sakthivelkumar S, Rajendran K, Janarthanan S. (2012). Screening of selected marine algae from the coastal Tamil Nadu, South India for antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 2(1): S140-S146.
28. Varier K M, Milton M, Arulvasu C, Gajendran B. (2013). Evaluation of antibacterial properties of selected red seaweeds from Rameshwaram, Tamil Nadu, India. Journal of Academia and Industrial Research, 1(11): 667-670.
29. Fazeli-Nasab B, Mirzaei N. (2018). Evaluation of total phenol and flavonoid content in a wide range of local and imported plants. Scientific Journal of Ilam University of Medical Sciences, 26(2): 141-154.
30. Kandhasamy M, Arunachalam K. (2008). Evaluation of in vitro antibacterial property of seaweeds of southeast coast of India. African Journal of Biotechnology, 7(12).
31. Taskin E, Caki Z, Ozturk M. (2010). Assessment of in vitro antitumoral and antimicrobial activities of marine algae harvested from the eastern Mediterranean sea. African Journal of Biotechnology, 9(27): 4272-4277.
32. Ghaffari M, Taheri A, Zobeidinezhad M. (2016). In vitro Evaluation of Antibacterial Effect of Ethyl Acetate Extract of Red Algae (Gelidiella acerosa) on Some Gram-positive and Gram-negative Bacteria. Journal of Rafsanjan University of Medical Sciences, 15(3): 209-222.
33. Rhimou B, Hassane R, José M, Nathalie B. (2010). The antibacterial potential of the seaweeds (Rhodophyceae) of the Strait of Gibraltar and the Mediterranean Coast of Morocco. African Journal of Biotechnology, 9(38): 6365-6372.
34. Kylli P, Nohynek L, Puupponen-Pimia R, Westerlund-Wikstrom B, Leppanen T, Welling J, Moilanen E, Heinonen M. (2011). Lingonberry (Vaccinium vitis-idaea) and European cranberry (Vaccinium microcarpon) proanthocyanidins: isolation, identification, and bioactivities. Journal of agricultural and food chemistry, 59(7): 3373-3384.
35. Lacombe A, Wu V C, White J, Tadepalli S, Andre E E. (2012). The antimicrobial properties of the lowbush blueberry (Vaccinium angustifolium) fractional components against foodborne pathogens and the conservation of probiotic Lactobacillus rhamnosus. Food microbiology, 30(1): 124-131.
36. Omar H, Shiekh H, Gumgumjee N, El-Kazan M, El-Gendy A. (2012). Antibacterial activity of extracts of marine algae from the Red Sea of Jeddah, Saudi Arabia. African Journal of Biotechnology, 11(71): 13576-13585.
37. Lavanya R, Veerappan N. (2011). Antibacterial potential of six seaweeds collected from Gulf of Mannar of southeast coast of India. Advances in Biological Research, 5(1): 38-44.
38. Malayeri F A, Yazdanpour Z, Bandani H, Fazeli-Nasab B, Saeidi S. (2020). Antimicrobial and anti-biofilm effects of Thyme essential oils and Peppermint on Acinetobacter baumannii and Staphylococcus aureus resistant to different antibiotics. New Findings in Veterinary Microbiology, 2(2): 41-51.
39. Fazeli-Nasab B, Fahmide L. (2020). Evaluation of Antioxidant Properties and Phenolic Compounds of Different Mango (Mangifera indica L.) Southern Iran. Crop Science Research in Arid Regions, 2(1): 11-21.
40. Manion C R, Widder R M. (2017). Essentials of essential oils. American Journal of Health-System Pharmacy, 74(9): e153-e162.
41. Aali E, Mahmoudi R, Kazeminia M, Hazrati R, Azarpey F. (2017). Essential oils as natural medicinal substances: review article. [Review Article]. Tehran University Medical Journal, 75(7): 480-489.
42. Nogueira J W A, Costa R A, da Cunha M T, Cavalcante T T A. (2017). Antibiofilm activity of natural substances derived from plants. African Journal of Microbiology Research, 11(26): 1051-1060.
43. Ribeiro-Santos R, Andrade M, Sanches-Silva A, de Melo N R. (2018). Essential oils for food application: natural substances with established biological activities. Food and Bioprocess Technology, 11(1): 43-71.
44. Noorhosseini S A, Fallahi E, Samizadeh M, Beheshtipoor N. (2017). The Relative Priority of Medicinal Plants, Herbal and Chemical Medicines by Consumers Based on Economic and Treatment Criteria: Case Study of Rasht District. Agricultural Economics Research, 9(33): 71-99.