Document Type : Original Article


1 Plant Protection Research Department, Ardabil Agricultural and Natural Resources Research and Education Centre, AREEO, Ardabil, Iran

2 Research Assistant Prof. Plant Breeding (Genetics-Biometry), Ardabil Agriculture and Natural Resources Research Centre, Ardabil, Iran



This study was performed in order to determine the distance of isolation for the seed potato production from ware potato in three regions of Ardabil, Namin and Nir. The experiment was carried out in a randomized complete block design (RCBD) with five distances (100, 200, 300, 400 and 500 meters from ware potato) and three replications with Super Elite (SE) class of Agria potato cultivar from ware potato as the source of infection studied by ELISA during 2014 and 2015. According to standard of Seed and Plant Certification and Registration Institute (SPCRI) number of 200 leaves and 200 tubers samples of each distance were collected and their virus in fection was by ELISA assay. Analysis of variance tuber yield showed that between regions, years and distances of isolation were significantly differences. In terms of tuber yield, between distances 500, 400 and 300 meters were not significantly difference and were in one group. With reducing of isolation distance from 500 meters to 100 meters from host of viral diseases vectors (edible potato of Agria cultivar) were decreased tuber yield from 44.328 ton per hectare to 39.828 ton per hectare (about 4.5 ton per hectare, or 10.15 percent). Average infection of Rhizoctonia fungal diseases of tuber in isolation distances and three regions were about 5 percent. In three regions and five isolation distances, tubers were without infection of vascular rot fungus diseases, without infection of bacterial diseases of blackleg and brown rot and without infection of potato moth pest.


Ahmadvand, R, Hassanabadi, H. (2009). Evaluation of Resistance of Potato Promising Clones to PVX, PVY and PVA in Greenhouse. Seed Plant Improv. J., 4(25):517-531.
Anonymous. (2005). Potato tissue culture minituber (PTMT) standard for certification. Shimla, India.
Beykzadeh N, Jafarpour B, Falahati Rastgar, M, Varasteh AAR. (2001). Investigation on Potato Virus X in North of Khorasan. J Agric Sci. 11(2): 7-21.
Bhutta, RA. (2008). Survey of tuber-borne diseases of potato in northern areas, Pakistan. Pakistan J. Phytopathol., 21:20-37. 
Bostan, H, Guclu, C, Oztuk, E, Ozdemir, I, Ilbagi, H. (2006). Influence of Aphids on the epidemiology of potato virus diseases (PVY, PVS and PLRV) in the high altitude areas of Turkey. Pakistan J. Biol. Sci., 9(4):759-765.
Buchen Osmond, C. (2002). ICTVdB: The Authorized Universal Virus Database. Biosphere 2 Center, Columbia University, Columbia.
De Boks, JDH. (1987). Viruses of potato and seed potato production. Wageningen, Netherlands. 259 pp.
Duan, G, Zhan, F,  Du, Z, Ho, SYW, Gaoa, F. (2018). Europe was a hub for the global spread of potato virus S in the 19th century. Virology., 525:200-204.
Haase, NU. (2008). The canon of potato science: 50. The nutritional value of potatoes. Potato Res., 50:415-417.
Hassanpanah, D. (2013). Evaluating Potential Production of Mid-Late Maturing Minituber of Potato Cultivars and Promising Clones under Aeroponic System. J. Crop Ecophysiol., 3(31):331-346.
Khurana, S. (2004). Potato viruses and their management. Diseases of Fruits and Vegetables. 2:389-440.
Lung’aho, C, Lemaga, B, Nyongesa, M, Gildermacher, P, Kinyae, P, Demo, P, Kabira, J. (2007). Commercial seed potato production in eastern and central Africa. Kenya Agricul. Res. Institute, (KARI)., pp1-140.
Mattews, REF. (1991). Plant virology. Academi Press, Inc. San Diego, CA, USA. p 835.
Mondal, S, Wenninger, EJ, Hutchinson, PJS, Whitworth, JL, Shrestha, D, Eigenbrode, SD, Bosque-Pérez, NA, Snyder, WE. (2017). Responses of Aphid Vectors of Potato leaf roll virus to Potato Varieties. Plant disease., 101(10):1812-1818.
Murphy, PA, Mckay, R. (1932). A Comparison of some European and American viruses disease in potato. Sci. Proceed. Royal Dublin Soc., 20:347-385.
Muthuraj, R. (2013). Pot seed certification. Division of Seed Technology, Central Potato Research Institute, Shimla. p 8.
Nasir, M, Zaidi, SSH, Batool, A, Hussain, M, Iqbal, B, Sajjad, M, Abbas, W, Javed, MM. (2012). ELISA-based detection of major potato viruses in tissue culture produced potato germplasm. Int. J. Agricult. Sci., 2(1):75-80.
Olubayo, F, Kibaru, A, Nderitu, J, Njeru, R, Kasina, M. (2010). Management of aphids and their vectored diseases on seed potatoes in Kenya using synthetic insecticides, mineral oil and plant extract. J. Innovat. Develop. Strat., 4(2):1-5.
Pazhouhandeh, M, Karvan, G, Razavi A. (2000). A review on potato genetic engineering researches yet. Genetic Engineering and Biosafety Journal. 6 (1) :175-188.
Ragsdale, D, Radcliffe, E, diFonzo, CD. (2001). Epidemiology and field control of PVY and PLRV. Kluwar Academic Publishers, Dordrecht, the Netherlands.
Rolot, JL. (2005). Analyse des Facteurs Régulant la Dissémination du Virus Y de la Pomme de Terre (Pvy) en Vue de Stratégies de Lutte Raisonnées. Gembloux, Belgium: Faculté des Sciences agronomiques de Gembloux, PhD thesis.
Salazar, LF. (1996). Potato viruses and their control. CIP Peru. p 214.
Valkonen, JPT. (2007). Viruses: economical losses and biotechnological potential. In: Vreugdenhil D, ed. Potato Biology and Biotechnology. New York, NY, USA: Elsevier, p 619–41