Inhibitory activity on tyrosinase and antioxidant activity of methanol extract of various aerial parts of Astragalus siliquosus Bioss. and Verbascum phoeniceum L.

Zarei, Mohammad Ali; Babaei, Masoumeh

doi:10.33945/SAMI/IJABBR.2020.3.3

Document Type : Original Article

Authors

Department of Biological Sciences, Faculty of Science, University of Kurdistan, Sanandaj, Iran

10.33945/SAMI/IJABBR.2020.3.3

Abstract

Background:
Melanogenesis begins with the oxidation of L-tyrosine to L-Dopa by tyrosinase. Inhibition of tyrosinase could be effective in treatment of complications such as skin lesions, eczema and melasma. Inhibition of tyrosinase could be effective in treatment of those complications. The aim of this study was to determine inhibitory activity on tyrosinase and antioxidant activity of Astragalus siliquosus Bioss. and Verbascum phoeniceum L.
Methods:
Methanol extracts of organs of the plants were prepared by maceration. Inhibitory effects of the extracts were evaluated in 96 wells at 492 nm wavelength. The antioxidant activity of the extracts was evaluated using DPPH free radical scavenging index and iron reduction test.
Results;
For A.siliquosus, maximum inhibitory activity was of its flower extract (97% inhibition and IC50=1.58 mg/ml) and of its stem extract (100 % and IC50=2.1 mg/ml). For V.phoeniceum, the highest inhibitory activity was of its leaf extract (87% inhibition and IC50=3.2 mg/ml). Flower extract of A.siliquosus showed mixed Uncompetitive-Non-competitive pattern of inhibitory activity, while the extract of the V.phoeniceum leaf showed mixed Competitive-Non-competitive inhibitory pattern. All aerial parts of the A.siliquosus had 100% antioxidant activity with EC50s of 0.089, 1.78, 1.25 mg/ml for its flower, leaf and stem respectively. Leaves of the V.phoeniceum had a 100% antioxidant activity, with EC50 of 0.013 mg/ml.
Conclusions:
Methanol extracts of flower and leaf organs of A.siliquosus, and leaf organ of V.phoeniceum, had a reasonable inhibitory effect on tyrosinase activity. Feature studies could be focused on those organs to separate potential agents with pharmaceutical and cosmetic applications.

Keywords

References

Arumugam, R, Kirkan, B, Sarikurkcu, C. (2019). Phenolic profile, antioxidant and enzyme inhibitory potential of methanolic extracts from different parts of Astragalus ponticus Pall. South Afr. J. Botany, 120:268-273.

Chung, YC, Chien, CT, Teng, KY, Chou, ST. (2006). Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & zucc. Food Chem., 97:418-425.

Fernandes, MS, Kerkar, S. (2017). Microorganisms as a source of tyrosinase inhibitors: a review. Annal. Microbiol., 67(4):343-3580.

Fu, R, Zhang, Y, Guo, Y, Chen, F. (2014). Antioxidant and tyrosinase inhibition activities of the ethanol-insoluble fraction of water extract of Sapium sebiferum (L.) Roxb. Leaves. South Afr. J. Botany, 93:98-104.

Hashemi, F, Zarei, MA., (2014). Tyrosinase Inhibitory Activity Within Hexane Extract of Ten Screened Plants from Kurdistan Province of Iran. Int. J. Adv. Biol. Biomed. Res., 2(11):2795-2799.

Hassani, A, Zarei, MA. (2019). Study of mushroom tyrosinase inhibitory activity among 70 plants from Kurdistan province. J. Med. Plants, In press.

Hinneburg, I, Dorman, HD, Hiltunen, R. (2006). Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem., 97:122-129.

Ito, S, Wakamatsu, K. (2008). Chemistry of mixed melanogenesis–Pivotal roles of dopaquinone. Photochem. Photobiol., 84:582–592.

Jablonski, NG, Chaplin, G. (2010). Human skin pigmentation as an adaptation to UV radiation. Proc. Natl. Acad. Sci., 107:8962–8968.

Jamshidi-Kia, F, Lorigooini, Z, Asgari, S, Saeidi, K. (2018). Iranian species of Verbascum: a review of botany, phytochemistry, and pharmacological effects. Toxin Rev., 38(4):255-262.

Khatib, S, Nerya, O, Musa, R. (2005). Chalcones as potent tyrosinase inhibitors: the importance of a 2, 4-substituted resorcinol moiety. Bioorg. Med. Chem., 13:433-441.

Lai, X, Wichers, HJ, Soler-Lopez, M, Dijkstr, BW. (2017). Structure and Function of Human Tyrosinase and Tyrosinase-related Proteins. Chem. A Eur. J., 24(1):47-55.

Lee, SY, Baek, N, Nam, TG. (2016). Natural, semisynthetic and synthetic tyrosinase inhibitors. J Enzyme Inhib. Med. Chem., 31(1):1–13.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 26:211-219.

Morakinyo Sanni, D, Omotoyinbo, OV. (2016). Inhibitory Effect of Extracts from Datura metel Leaf on Mushroom Tyrosinase. Am. J. Life Sci., 4(2):47-50.

Palmer, T, Bonner PL. (2007). ENZYMES: Biochemistry, Biotechnology and Clinical Chemistry. Second edition published by Horwood Publishing Limited. Reprinted by Woodhead Publishing Limited, 2011, Philadelphia, PA 19102-3406, USA.

Rolff, M, Schottenheim, J, Decker, H, Tuczek, F. (2011). Copper–O2 reactivity of tyrosinase models towards external monophenolic substrates: Molecular mechanism and comparison with the enzyme. Chem. Soc. Rev., 40: 4077–4098.

Sanandaji, S, Mozaffarian, V. (2010). Studies of Flora in Saral area: Kurdistan–Iran. Taxonomy Biosystemat., 3:59-84.

Sánchez-Ferrer, A, Rodríguez-López, JN, García-Cánovas, F, García-Carmona, F. (1995). Tyrosinase: a comprehensive review of its mechanism. Biochim. Biophys. Acta, 1247(1):1–11.

Shukla, S, Park, P, Kim, DH, Hong, SY, Lee, JS, Kim, M. (2016). Total phenolic content, antioxidant, tyrosinase and a-glucosidase inhibitory activities of water soluble extracts of noble starter culture Doenjang, a Korean fermented soybean sauce variety. Food Control, 59:854-861.

Siahpoosh, A, Amraee, F. (2011). Antioxidant Capacity of Various Extracts of Asteragalus Morinus Boiss Aerial Parts. J. Shahid Sadoughi University of Med. Sci., 19(4):437-444.

Solomon, EI, Sundaram, UM, Machonkin, TE. (1996). Multicopper oxidases and oxygenases. Chem. Rev., 96:2563–2606.

Verbascum phoeniceum L. in GBIF Secretariat (2019). GBIF Backbone Taxonomy. Checklist dataset https://doi.org/10.15468/39omei accessed via GBIF.org on 2019-11-27.

Yang, HH, Oh, KE, Jo, YH, Ahn, JH, Liu, Q, Turk, A, Jang, JY, Hwang, BY, Lee, KY, Lee, MK. (2017). Characterization of tyrosinase inhibitory constituents from the aerial parts of Humulus japonicus using LC-MS/MS coupled online assay. Bioorg. Med. Chem., 26:509–515.

International Journal of Advanced Biological and Biomedical Research

Inhibitory activity on tyrosinase and antioxidant activity of methanol extract of various aerial parts of Astragalus siliquosus Bioss. and Verbascum phoeniceum L.

References

References

Volume 8, Issue 3
September and October 2020
Pages 242-252

Inhibitory activity on tyrosinase and antioxidant activity of methanol extract of various aerial parts of Astragalus siliquosus Bioss. and Verbascum phoeniceum L.

References

References

Volume 8, Issue 3September and October 2020Pages 242-252

Volume 8, Issue 3
September and October 2020
Pages 242-252