Document Type : Original Article


1 Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, 11365-8639, Iran

2 2School of Chemical Engineering, College of Engineering, University of Tehran, Iran

3 1Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, 11365-8639, Iran



The role of heart disease in increasing worldwide death and the limited availability of organs for transplantation have encouraged multiple strategies to fabricate functional and implantable constructs. One of these strategies is to develop a biologically similar heart tissue scaffold, in which two types of fiber and hydrogel are commonly used. Toward this goal, taking advantage of both hydrogels properties and fibers features with excellent mechanical properties can be considered as a promising method. The purpose of this study is to develop a fiber/hydrogel composite of gelatin, poly-caprolactone (PCL), cardiac extracellular matrix (ECM), and chitosan. The fibrous scaffolds of PCL and gelatin were characterized by SEM, water drop contact angle test, FTIR, and mechanical tests. The results showed that the average diameter of nanofibers, hydrophilicity and mechanical properties of the fibrous scaffolds increased with increasing the gelatin content in the spinning solution. Furthermore, the results of mechanical tests indicated that by integrating fibers with gelatin to PCL mass ratio of 2 in the hydrogel of chitosan and ECM with a mass ratio equal to 1, we obtained a construct with similar mechanical properties to native heart tissue, which may be proposed as an appropriate scaffold for heart tissue engineering.


Main Subjects

Aghdam Mehdinavaz, R, Shakhesi, S, Najarian,S, Malek Mohammadi, M, Ahmadi Tafti, SH, Mirzadeh, H. (2014). Fabrication of a Nanofibrous Scaffold for the In Vitro Culture of Cardiac Progenitor Cells for Myocardial Regeneration. Int. J. Polym. Mater. Polym. Biomater., 63:229–239.
Ahn, S, Ardoña, HAM, Lind, JU, Eweje, F, Kim, SL, Gonzalez, GM, Liu, Q, Zimmerman, JF, Pyrgiotakis, G, Zhang, Z, Beltran-Huarac, J,
Carpinone, P, Moudgil, BM, Demokritou, P, Parker, KK. (2018). Mussel-Inspired 3D Fiber Scaffolds for Heart-on-a-Chip Toxicity Studies of Engineered Nanomaterials. Anal. Bioanal. Chem., 410:6141–6154.
Binulal, NS, Natarajan, A, Menon, D, Bhaskaran, VK, Mony, U, Nair, SV. (2014). PCL–Gelatin Composite Nanofibers Electrospun Using Diluted Acetic Acid–Ethyl Acetate Solvent System for Stem Cell-Based Bone Tissue Engineering. J. Biomater. Sci. Polym. Edit., 25(4):325–340.
Chong, E, Phan, T, Lim, I, Zhang, Y, Bay, B, Ramakrishna, S, Lim, C. (2007). Evaluation of Electrospun PCL/Gelatin Nanofibrous Scaffold for Wound Healing and Layered Dermal Reconstitution. Acta Biomater., 3(3):321–330.
Yeyzon, C, Muñoz, E, Gomez-Pachón, EY, Morales-Corona, J, Olayo-Lortia, J, Olayo, R, Olayo-Valles, R. (2019). Electrospun PCL-Protein Scaffolds Coated by Pyrrole Plasma Polymerization. J. Biomater. Sci. Polym. Edit., 30:832-845.
Piotr, D, Dulnik, J, Sajkiewicz, P. (2015). Electrospinning and Structure of Bicomponent Polycaprolactone/Gelatin Nanofibers Obtained Using Alternative Solvent System. Int. J. Polym. Mater. Polym. Biomater., 64(7):354–364.
Dunn, DA, Alexander JH, Lipke, EA. (2014). Biomimetic Materials Design for Cardiac Tissue Regeneration. Wiley Interdisciplin. Rev. Nanomed. Nanobiotechnol., 6(1):15–39.
Esmaeili Pourfarhangi, K, Mashayekhan, S, Ghanbari Asl, S, Hajebrahimi, Z. (2018). Construction of Scaffolds Composed of Acellular Cardiac Extracellular Matrix for Myocardial Tissue Engineering. Biologicals, (J. Int. Associat. Biolog. Standardizat.,) 53:10–18.
Gautam, S, Dinda, AK, Mishra, NC. (2013). Fabrication and Characterization of PCL/Gelatin Composite Nanofibrous Scaffold for Tissue Engineering Applications by Electrospinning Method.” Mater. Sci. Eng. C, 33(3):1228–1235.
Hu, T, Wu, Y, Zhao, X, Wang, L, Bi, L, Ma, PX, Guo, B. (2019). Micropatterned, Electroactive, and Biodegradable Poly(Glycerol Sebacate)-Aniline Trimer Elastomer for Cardiac Tissue Engineering. Chem. Eng. J., 366:208–222.
Ismail, HM, Zamani, S, Elrayess, MA, Kafienah, W, Younes, HM. (2018). New Three-Dimensional Poly(Decanediol-Co-Tricarballylate) Elastomeric Fibrous Mesh Fabricated by Photoreactive Electrospinning for Cardiac Tissue Engineering Applications. Polymers. 10(4):455.
Jafarkhani, M, Salehi, Z, Kowsari-Esfahan, R, Shokrgozar, MA, Mohammadi, MR, Rajadas, J, Mozafari, M. (2018). Strategies for Directing Cells into Building Functional Hearts and Parts. Biomater. Sci., 6(7):1664–1690.
Jin, J, Jeong, SI, Shin, YM, Lim, KS, Shin, HS, Lee, YM, Koh, HC, Kim, KS. (2009). Transplantation of Mesenchymal Stem Cells within a Poly(Lactide- Co -ɛ-Caprolactone) Scaffold Improves Cardiac Function in a Rat Myocardial Infarction Model. Eur. J. Heart Failure, 11(2):147–153.
Jose, MV, Thomas, V, Dean, DR, Nyairo, E. (2009). Fabrication and Characterization of Aligned Nanofibrous PLGA/Collagen Blends as Bone Tissue Scaffolds. Polymer, 50(15):3778–3785.
Kucinska-Lipka, J, Gubanska, I, Janik, H, Sienkiewicz, M. (2015). Fabrication of Polyurethane and Polyurethane Based Composite Fibres by the Electrospinning Technique for Soft Tissue Engineering of Cardiovascular System. Mater. Sci. Eng. C, 46:166–176.
Meechaisue, C, Dubin, R, Supaphol, P, Hoven, VP, Kohn, J. (2006). Electrospun Mat of Tyrosine-Derived Polycarbonate Fibers for Potential Use as Tissue Scaffolding Material. J. Biomater. Sci. Polym. Edit., 17(9):1039–1056.
Nasiri, B, Mashayekhan, S. (2017). Fabrication of Porous Scaffolds with Decellularized Cartilage Matrix for Tissue Engineering Application. Biologicals, 48:39–46.
Pereira, IHL, Ayres, E, Averous, L, Schlatter, G, Hebraud, A, Ana CCP, Viana, PHL, Goes, AM, Oréfice, RL. (2014). Differentiation of Human Adipose-Derived Stem Cells Seeded on Mineralized Electrospun Co-Axial Poly(ε-Caprolactone) (PCL)/Gelatin Nanofibers. J. Mater. Sci. Mater. Med., 25(4):1137–1148.
Pomeroy, JE, Helfer, A, Bursac, N. (2019). Biomaterializing the Promise of Cardiac Tissue Engineering. Biotechnol. Adv., February.
Rajabi-Zeleti, S, Jalili-Firoozinezhad, S, Azarnia, M, Khayyatan, F, Vahdat, S, Nikeghbalian, S, Khademhosseini, A, Baharvand, H,  Aghdami, N. (2014). The Behavior of Cardiac Progenitor Cells on Macroporous Pericardium-Derived Scaffolds. Biomaterials, 35(3):970–982.
Safaeijavan, R, Safaeijavan, R, Soleimani, M, Divsalar, A, Eidi, A, Ardeshirylajimi, A. (2013). Biological Behavior Study of Gelatin Coated PCL Nanofiberous Electrospun Scaffolds Using Fibroblasts. J. Paramed. Sci., 5(1).
Siddiqui, N, Asawa, S, Birru, B, Baadhe, R, Rao, S. (2018). PCL-Based Composite Scaffold Matrices for Tissue Engineering Applications. Mol. Biotechnol., 60(7):506–532.
Singelyn, JM, DeQuach, JA, Seif-Naraghi, SB, Littlefield, RB, Schup-Magoffin, PJ,  Christman, KL. (2009). Naturally Derived Myocardial Matrix as an Injectable Scaffold for Cardiac Tissue Engineering. Biomaterials, 30(29):5409–5416.
Sivandzade, F, Mashayekhan, S. (2018). Design and Fabrication of Injectable Microcarriers Composed of Acellular Cartilage Matrix and Chitosan. J. Biomater. Sci. Polym. Edit., 29(6):683–700.
Su, WF, Ho, CC, Shih, TH, Wang, CH, Yeh, CH. (2016). Exceptional Biocompatibility of 3D Fibrous Scaffold for Cardiac Tissue Engineering Fabricated from Biodegradable Polyurethane Blended with Cellulose. Int. J. Polym. Mater. Polym. Biomater., 65(14):703–711.
Vunjak-Novakovic, G, Tandon, N, Godier, A, Maidhof, R, Marsano, A, Martens, TP, Radisic, M. (2010). Challenges in Cardiac Tissue Engineering. Tissue Eng. B Rev., 16(2):169–187.
Yang, Y, Wang, C, Wiener, CG, Hao, J, Shatas, S, Weiss, RA, Vogt, BD. (2016). Tough Stretchable Physically-Cross-Linked Electrospun Hydrogel Fiber Mats. ACS Appl. Mater. Interfac., 8(35):22774–22779.
Yang, Y, Wimpenny, I, Ahearne, M. (2011). Portable Nanofiber Meshes Dictate Cell Orientation throughout Three-Dimensional Hydrogels. Nanomed. Nanotechnol. Biol. Med., 7(2):131–136.
Zhang, Y, Ouyang, H, Lim, CT, Ramakrishna, S, Huang, ZM. (2005). Electrospinning of Gelatin Fibers and Gelatin/PCL Composite Fibrous Scaffolds. J. Biomed. Mater. Res., 72B(1):156–165.
Zhao, P, Jiang, H, Pan, H, Zhu, K, Chen, W. (2007). Biodegradable Fibrous Scaffolds Composed of Gelatin Coated Poly(ε-Caprolactone) Prepared by Coaxial Electrospinning. J. Biomed. Mater. Res. A, 83A(2):372–382.