Document Type : Original Article


Young Researchers ans Elite Club, Jiroft Branch, Islamic Azad University, Jiroft Branch, Iran


Effect of gibberellic acid on marigold (Calendula Officinalis L.) was evaluated in a pot culture experiment. A factorial experiment based on completely randomized design including 12 treatments and four replications was carried out. Main factor was foliar application stages (first, second and third) and sub factor included different concentrations of GA3 (0, 50, 150 and 250 mg L-1). Results showed that foliar application of GA3 had positive effect on photosynthetic pigments. Effect of different concentrations of GA3 on chlorophyll a was significant (p<0.01). Chlorophyll a content was enhanced by increase in GA3 concentration up to 250 mg L-1 treatment of 250 mg L-1 resulted in production of 7.78‌ µg/L-1 chlorophyll a, the index which was to some extent dropped in other concentrations. Different concentrations of GA3 had significant effect on chlorophyll b (p<0.01). Chlorophyll b was increased by increase in GA3 concentration up to 250 mg L-1. the highest rate of total chlorophyll content and total pigment in three times of application and one application of 250 mg L-1 was 14.6 and 15.4 µg/L-1 respectively; whereas the lowest chlorophyll and pigment content was observed in one foliar application of control treatment with mean value as 4.67 and 5.5 µg/L-1.


Azuma. T., Ueno, S., Uchid, N and Yasuda, T. (1997). Gibberellin induced elongation and osmoregulation in internodes of floating rice. Physiologia Plantarum, 99: 517ñ522.
Bedour, H., Abou-Leila, M.S and Abdel-Hady, N.F. (1994). Effect of foliar application of GA3 and Zn on Ocimum basillicum L. grown in different soil type. Egypt. J. Physiol. Sci., 18: 365-380.
Dahan, K., Fennal, M and Kumar, N.B. (2008). Lycopene in the prevention of prostate cancer.J Soc Integrat Oncol, 6: 29-36.
Eraki, M.A. (1994). The effect of gibberellic application and chelated iron nutrition on the growth and flowering of Queen Elizabeth rose plants. The first Conf. of Ornamental Hort., 2: 436-444.
Gazim, Z.C., Rezende C.M., Fraga, S.R., Svidzinski, T.I.E and Cortez, D.A.G. (2008). Antifungal activity of the essential oil from Calendula officinalis L. (Asteraceae) growing in Brazil. Braz J Microby, 39: 61-3.
Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods of Enzymology 148: 350-380.
Pintea, A., Bele, C., Andrei, S and Socaciu, C. (2003). HPLC analysis of carotenoids in four varieties of Calendula officinalis L. flowers. Acta Biologica Szegediensis, 47: 37-40.
Rahbarian, P, Salehi Sardoei, A and Fallah Imani, A. (2014). Stimulatory Effect of benzyladenine and gibberellic acid on Growth and Photosynthetic pigments of (Spathiphyllum wallisii Regel) Plants. International journal of Advanced Biological and Biomedical Research. 2(1): 230-237.
SAS. (2002). SAS/STATÆ userís guide, SAS Institute, Cary, NC, USA. 9.1.
Salehi Sardoei, A., rahbarian, P and Fallah Imani, A. (2014a). Stimulatory Effect of gibberellic acid and benzyladenine on Growth and Photosynthetic pigments of Ficus benjamina L. Plants. International journal of Advanced Biological and Biomedical Research. 2(1): 34-42.
Salehi Sardoei, A. (2014b). Response of Application Gibberellic acid (GA3) and Benzyladenine (BA) to Dizigotheeca elegantissima Plants. International journal of Advanced Biological and Biomedical Research. 2(3): 615-621.
Salehi Sardoei, A. (2014c). Plant Growth Regulators Effects on the Growth and Photosynthetic pigments on Three indoor ornamental Plants. International European Journal of Experimental Biology. In PressSalehi Sardoei &
Shedeed, M.R., Gamassy, K.M., Hashim, M.E and Almulla, A.M.N. (1991). Effect of fulifirtil fertilization and growth regulators on the vegetative growth of croton plants. Annals Agric. Sci., Ain. Shams Univ., Cairo., 36: 209-216.
Teszl¥ak, P., Ga¥al, K and Nikfardjam, M. (2005). Influence of grapevine flower treatment with gibberellic acid (GA3) on polyphenol content of Vitis vinifera L. wine. Analytica Chimica Acta, 54: 275ñ28.