Document Type : Original Article


Department of Microbiology, Faculty of Basic Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran


Objective: Due to the alarming increase the incidence and prevalence of infectious diseases and increasing bacterial resistance to antibiotics need to discover new strong antimicrobial compounds is feeling.Methods: In this study, the antimicrobial properties of spider silk Pholcus phalangioides produced in sterile conditions against two bacteria, Listeria monocytogenes and Esherichia coli with using well diffisiuon method and Macro Broth Dillution method was evaluated. Results: The results showed that the antimicrobial compounds present in the solution spider silk greater inhibitory effect on gram-positive bacteria L. monocytogenes than Gram-negative bacteria E. coli.


Chakraborty, D.S., 2009. Antibacterial activities of cobweb protein. European Congress of Clinical Microbiology and Infectious Diseases.
Gomes, S.C, Leonor, I., B, Mano, J.F., 2010. Functionalized
silk biomaterials for bone regeneration. Semana de engenharia. PP: 1-2.
Gellynck, K., Kiekens, P., Mertens, J., 2006. Coconzijde en
spinrag in weefselengineering silk and spider silk in tissue engineering. Academiejaar. PP: 1-220.
Heimer, S., 1988. Wunderbare Welt der Spinnen, Urania. Mohamed, E.S. Mirghani., Kabbashi, N.A., Efaki, F.A., M. Z. Fahmi B. Zulkifli., 2012. Investigation of the spider web for antibacterial activity. Malaysian International Conference on Trends in Bioprocess Engineering. 201, 1-5.
May, T.B., Shinabarger, D., Mahara, J., 2000. Alginat synthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infection of cystic fibrosis patients. Clinical Microbiology Review. 2, 191-192.
Nelmerk, W., 2005. National committee for clinical laboratory standards. Approved standard method for dilution antimicrobial susceptibility test for bacteria, 3, 278-294.
Zuridah, H., Fairoz, A.R.M., Zakri, A.H.Z., Rahim, M.N.A., 2008. In vitro antibacterial activity of Nigell sativa against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus. Asian Journal of Plant Sciences. 3, 331-333.