1 Division Microbiology,Department of Molecular and Cell biology, Faculty of Basic Sciences, Lahijan Branch,Islamic Azad University(IAU), Lahijan, Iran

2 Division Mycology&parasitology, Department of Pathobiology, Faculty of Veterinary Medical Sciences, Science & Research Branch, Islamic Azad University (IAU),Tehran, Iran


Objective: Citrinin is a mycotoxin produced by several species of the genera Aspergillus and Penicillium and occurs mainly in food products and animal feed. Some scientific reports show a link between Citrinin and nephrotoxic and possibly a carcinogenic effect for humans. Methods: Samples collected by settling plates,in northern Iran and pure culture isolation performed till the toxin measurement to be done in cell extracts (biomass) which prepared by merging culture in separated prepared culture media incubation. The amount of toxin measured by extracting solutions (50% Acetone, 25% Alcohol Methylic,25% Alcohol Ethylic and PBS washing proposed) using Direct Competitive ELISA. Results: In conducted research, the relative distribution per obtained Aspergillus species isolates for biomass preparation showed that the most frequent were A.ostianus, A.fumigatus,A.niveus,A.niger,A.awamori and A.parasiticus respectively with a prevalence (28.6%), according to averaged Citrinin, indicated that the potent species was A.niger (2009.3ppb) whereas the lowest observed by A.wentii (18.46 ppb).According to maximum Citrinin limits (200ppb) in food products  and animal feed including especially related to the Aspergilli and Penicilli, as the result of our measurements and the performed statistical analysis , the maximum amount of Citrinin in the cell extracts, respectively were  determined (2009.3ppb) and always produced by A.niger , that were too much more than the universal and local standards allowance enable us to introduce it for all biotechnologic or toxicologic research mentions eventhought as a scientific opinion on the risks for public and animal health related to the presence of Citrinin in food and feed at the targeted geographic area.


Main Subjects

Ambrose AM and DeEds F, 1946. Some toxicological and pharmacological properties of citrinin. The Journal of Pharmacology and Experimental Therapeutics, 88, 173- 186.
Aziz NH, Mattar ZA and Mahrous SR, 2006. Contamination of grains by mycotoxin-producing molds and mycotoxins and control by gamma irradiation. Journal of Food Safety, 26, 184-201.
Dietrich R, Schmid A and M‰rtlbauer E, 2001. Citrinin in fruit juices. Mycotoxin Research, 17, 156-159.
Du L, Li D, Zhang G, Zhu T, Ai J and Gu Q, 2010. Novel carbon-bridged citrinin dimers from a volcano ashderived fungus Penicillium citrinum and their cytotoxic and cell cycle arrest activities. Tetrahedron, 66, 9286-9290.
Duan ZH, Lin ZS, Yao HR, Gao YH, Zhang K, Zhao SQ and Zhu ZY, 2009. Preparation of artificial antigen and egg yolk-derived immunoglobulin (IgY) of citrinin for enzyme-linked immunosorbent assay. Biomedical and
Environmental Sciences, 22, 237-243. 
EFSA (European Food Safety Authority), 2011a.
Evaluation of the FoodEx, the food classification system applied to the development of the EFSA Comprehensive European Food Consumption Database. EFSA Journal,9(3):1970, 27 pp.
EFSA (European Food Safety Authority), 2011b. Use of the EFSA Comprehensive European Food Consumption Database in Exposure Assessment. EFSA Journal 2011;9(3):2097, 34 pp.
Franco CM, Fente CA, Vazquez B, Cepeda A, Lallaoui L, Prognon P and Mahuzier G, 1996. Simple and sensitive high-performance liquid chromatography--fluorescence method for the determination of citrinin application to the analysis of fungal cultures and cheese extracts.
Journal of Chromatography, A, 723, 69-75.
Hussein S, Brasel JM, 2001.Toxicity, metabolism and impact of mycotoxins on humans and animals,Toxicology, 167 (2001), pp. 101ñ134.
IARC (International Agency for Research on Cancer),1993. Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Human: Some
Naturally Occurring Substances. Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins (Lyon : IARC, pp. 397ñ444.
Kononenko GP and Burkin AA, 2008. A survey on the occurrence of citrinin in feeds and their ingredients in Russia. Mycotoxin Research, 24, 3-6.
Li Y-N, Wang Y-Y, Zheng Y-Q and Guo Y-H, 2010. Preparation and Characterization of The High Specificity Monoclonal Antibodies Against Citrinin. Progress in Biochemistry and Biophysics, 37, 1248-1253.
Nguyen MT, Tozovanu M, Tran TL and Pfohl-Leszkowicz A , 2007. Occurrence of aflatoxin B1, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam. Food Chemistry, 105, 42-47.
Polisenska I, Pfohl-Leszkowicz A, Hadjeba K, Dohnal V, Jirsa O, Denesova O, Jezkova A and Macharackova P, 2010.Occurrence of ochratoxin A and citrinin in Czech cereals and comparison of two HPLC methods for ochratoxin A detection. Food Additives and Contaminants, A, 27, 1545-1557.
Poupko R, Luz Z and Destro R, 1997. Carbon-13 NMR of citrinin in the solid state and in solutions. Journal of Physical Chemistry A, 101, 5097-5102.
Santos L, Marin S, Sanchis V and Ramos AJ, 2009. Screening of mycotoxin multicontamination in medicinal and aromatic herbs sampled in Spain. Journal of the Science of Food and Agriculture, 89, 1802-1807.
Subrahmanyam P and Rao AS, 1974. Occurrence of aflatoxins and citrinin in groundnut (Arachis hypogaea L.) at harvest in relation to pod condition and kernel moisture content. Current Science, 43, 707-710.
Tabata S, Iida K, Kimura K, Iwasaki Y, Nakazato M, Kamata K and Hirokado M, 2008. Investigation of ochratoxin A, B and citrinin contamination in various
commercial foods. Journal of the Food Hygienic Society of Japan, 49, 111-115.
Vrabcheva T, Usleber E, Dietrich R and M‰rtlbauer E, 2000. Co-occurrence of ochratoxin A and citrinin in cereals from Bulgarian villages with a history of Balkan endemic nephropathy. Journal of Agricultural and Food
Chemistry, 48, 2483-2488.
Xu BJ, Jia XQ, Gu LJ and Sung CK, 2006. Review on the qualitative and quantitative analysis of the mycotoxin citrinin. Food Control, 17, 271-285.