Document Type : Original Article


1 Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

2 Ferdowsi University of Mashhad, International Campus, Mashhad, Iran


Objective: In order to Investigate effects of Gibberlic acid (GA3) and Sprimidin Poly Amine on Germination of three Cultivars of Wheat under Salinity Stress. Methods: The experiment was conducted as factorial based on completely randomized design with four replicates in Agronomy and plant breeding laboratory of Agricultural department of Islamic Azad University, Varamin-Pishva branch in spring of 2014. The first factor was salinity with four levels (S1: 3.87 ds/m, S2: 5.95 ds/m, S3: 10.25 ds/m and S4: 12.87 ds/m) and the second factor was three cultivars of spring wheat including V1: Tajan, V2: Arg and V3: Pishtaz and the third factor 4 levels of hormone including H0: control (not application of hormone), H1: GA3 100 mg/L, H2: sprimidin 5 mmol/L and H3: GA3 100 mg/L + sprimidin 5 mmol/L. coleoptile and cleorhiza length, cleorhiza number, coleoptiles, cleorhiza and seedling dry weight, tissue water content (TWC), germination percent and number of damaged seeds were measured. Results: Analysis of variance showed that coleoptile and cleorhiza length, cleorhiza number, seedling and cleorhiza dry weigth affected by salinity, hormone and cultivar treatment at 1% of probability level. There are no significant differences for other properties. Results of this experiment indicated that even application of these treatments, development processes of germination under salinity stress decreased greatly.


Afzal I, Basra S, Iqbal A, 2005. The Effect of Seed Soaking With Plant Growth Regulators on Seedling Vigor of Wheat Under Salinity Stress. J. Stress Physiol Biochem. 1:6-14
Akhiyarova GR, Sabirzhanova IB, Veselov DS, Frike V ,2005. Participation of plant hormones in growth resumption of wheat shoots following short-term NaCl treatment. Rus J Plant Physiol52: 788-792.
Akman Z , 2009. Effects of GA3 and kinetin pre-sowing treatments on seedling emergence and seedling growth in wheat under saline conditions. J. Anim. Vet. Adv. 8:362-367.
Angelov G. B, 2003. Isoenzyme variation of esterase and acid phosphatase and genetic affinities among Dasypyrum villosum. Turkish Journal of Botany, 27, 249-254.
AfzalI, Basra S, Iqbal A, 2005. The Effect of Seed Soaking With Plant Growth Regulators on Seedling Vigor of Wheat Under Salinity Stress. J. Stress Physiol Biochem. 1:6-14
AtiaA, Debez A, Barhoumi Z, Smaoui A, AbdellyC, 2009. ABA, GA3 , and nitrate may control seed germination of Crithmum maritimum (Apiaceae) under saline conditions. C.R. Biol. 332, 704–710.
Basalah MO, Mohammad S , 1999. Effect of salinity and plant growth regulators on seed germination of Medicagosativa L. Pakistan J Biol Sci 2: 651-653
Bouchereau A, Aziz A, Larher F, Martin-Tanguy J, 1999. Polyamines and environ- mental challenges. Recent Dev. Plant Sci. 140, 103–125.
Das C, Sengupta T, Chattopadhyay S, Setua M, Das NK, Saratchandra B, 2002. Involvement of kinetin and spermidine in controlling salinity stress in mulberry (Morus alba L. cv. S-1). Acta Physiol Plant 24: 53-57
Groppa M. D, Benavides M. P, 2008. Polyamines and abiotic stress: recent advances. Amino Acids 34: 35-45.
Hanzawa Y, Takahashi T, Michael A.J, Burtin D, Long D, Pineiro M, CouplandG,KomedaY, 2000. ACAULIS5, an Arabidopsis gene required for stem elongation, encodes a spermine synthase. EMBO J. 19, 4248–4256.
HareP. D, W.A Cress , J. van Staden, 1997. The involvement of cytokinin in plant responses toenvironmental stress. Plant Growth Regulation. 23: 79–103
HisamatsuT, 2000. The role of gibberelllin in biosynthesis in the control of growth and flowering in Mathiola incana. Physiol. Plant., 109, 97-105.
Hollington P.A, 2000. Technological aL through sinscreening/breeding wheat varieties for salt tolerance. In: Gupta, S.K., Sharma, S.K., Tyagi, N.K. (Eds.),
Pro- ceedings National Conference on Salinity Management in Agriculture, December Iqbal M, Ashraf M, 2005a. Changes in growth,
photosynthetic capacity and ionic relations in spring wheat (Triticum aestivum L.) due to pre-sowing seed treat- ment with polyamines. Plant Growth Regul. 46,19–30
Iqbal M, Ashraf M, 2006. Wheat seed priming in relation to salt tolerance: growth, yield and levels of free salicylic acid and polyamines. Ann. Bot. Fenn. 43, 250–259.
Iqbal M, Ashraf M , 2010. Gibberellic Acid Mediated Induction of Salt Tolerance in Wheat Plants: Growth, Ionic Partitioning, Photosynthesis, Yield and Hormonal Homeostasis. Environ. Exp. Bot. doi:10.1016/j.envexpbot.
2010.06. 02.
Ismail AM , 2003. Physiological studies on the influence of cytokinin or GA3 in the alleviation of salt stress in sorghum plants. Acta Agro. Hung. 51:371-380.
Kasinathan V,Wingler A, 2004. Effect of reduced arginine decarboxylase activity on salt tolerance and on polyamine formation during salt stress in Arabidopsis thaliana. Physiol. Plant 121, 101–107.
Liu J. H, Inoue H, MoriguchiT, 2008. Salt stress-induced changes in free polyamine titers and expression of genes responsible for polyamine biosynthesis of apple in vitro shoots. Environmental Experimental Botany62: 28-35.
Moschou P.N, Paschalidis K.A, Roubelakis-Angelakis K.A, 2008. Plant polyamine catabolism: The state of the art.
Plant Signal. Behav. 3, 1061–1066.Olszewski, N., Sun, T.P., Gubler, F., 2002. Gibberellin signaling, biosynthesis, atabolism, and response pathways. Plant Cell 14, S61–S80.
Ranjan R, Purohit S , Prasad V , 2003. Plant Hormone: Action and Application. Agrobios(India). 243P.
Sarapatka B, Dudova L, Kroskova M,2004. Effect of pH and phosphate supply on acid phosphatase activity in cereal roots. Biologia Bratislova, 59, 127-131.
Stavir K,. Gupt A. K , Kaure N, 2000. Gibberelic Acid and kinetin partially reverse the effect of water stress on germination and seedling growth in chick pea. Plant growth regulation. 25: 29-33.
Tang W, Newton J. R, 2005. Polyamines reduced salt induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in Virginia pine. Plant Growth Regulation 46: 31-43.
Trewavas A, 1981. How Do Plant Growth Substances Work? Plant, Cell Environ. 4: 203-228
TrewavasA, Cleland R. E, 1983. Is Plant Development Regulated By Changes In The Concentration Of Growth Substances Or By Changes In The Sensitivity To Growth Substances Trends Biochem. Sci. 8:354-357.